Abundance and diversity of diazotrophs in the surface sediments of Kongsfjorden, an Arctic fjord
Diazotrophy in the Arctic environment is poorly understood compared to tropical and subtropical regions. Hence in this study, we report the abundance and diversity of diazotrophs in Arctic fjord sediments and elucidate the role of environmental factors on the distribution of diazotrophs. The study w...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2021-03, Vol.37 (3), p.41-41, Article 41 |
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| creator | Jabir, T. Vipindas, P. V. Krishnan, K. P. Mohamed Hatha, A. A. |
| description | Diazotrophy in the Arctic environment is poorly understood compared to tropical and subtropical regions. Hence in this study, we report the abundance and diversity of diazotrophs in Arctic fjord sediments and elucidate the role of environmental factors on the distribution of diazotrophs. The study was conducted during the boreal summer in the Kongsfjorden, an Arctic fjord situated in the western coast of Spitsbergen. The abundance of
nifH
gene was measured through quantitative real-time PCR and the diversity of diazotrophs was assessed by
nifH
targeted clone library and next generation sequence analysis. Results revealed that the abundance of
nifH
gene in the surface sediments ranged from 2.3 × 10
6
to 3.7 × 10
7
copies g
− 1
. The δ-proteobacterial diazotrophs (71% of total sequence) were the dominant class observed in this study. Major genera retrieved from the sequence analysis were Desulfovibrionaceae (25% of total sequence), Desulfuromonadaceae (18% of total sequence) and Desulfobacteriaceae (10% of total sequence); these are important diazotrophic iron and sulfur-reducing bacterial clade in the Kongsfjorden sediments. The abundance of
nifH
gene showed a significant positive correlation TOC/TN ratio (r
2
= 0.96, p ≤ 0.05) and total organic carbon (p ≤ 0.05) content in the fjord sediments. The higher TOC/TN ratio (4.24–14.5) indicated low nitrogen content organic matter in the fjord sediments through glacier runoff, which enhances the abundance and diversity of nitrogen fixing microorganisms.
Graphic abstract |
| doi_str_mv | 10.1007/s11274-020-02993-1 |
| format | Article |
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nifH
gene was measured through quantitative real-time PCR and the diversity of diazotrophs was assessed by
nifH
targeted clone library and next generation sequence analysis. Results revealed that the abundance of
nifH
gene in the surface sediments ranged from 2.3 × 10
6
to 3.7 × 10
7
copies g
− 1
. The δ-proteobacterial diazotrophs (71% of total sequence) were the dominant class observed in this study. Major genera retrieved from the sequence analysis were Desulfovibrionaceae (25% of total sequence), Desulfuromonadaceae (18% of total sequence) and Desulfobacteriaceae (10% of total sequence); these are important diazotrophic iron and sulfur-reducing bacterial clade in the Kongsfjorden sediments. The abundance of
nifH
gene showed a significant positive correlation TOC/TN ratio (r
2
= 0.96, p ≤ 0.05) and total organic carbon (p ≤ 0.05) content in the fjord sediments. The higher TOC/TN ratio (4.24–14.5) indicated low nitrogen content organic matter in the fjord sediments through glacier runoff, which enhances the abundance and diversity of nitrogen fixing microorganisms.
Graphic abstract</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-020-02993-1</identifier><identifier>PMID: 33544264</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abundance ; Applied Microbiology ; Arctic environments ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Environmental Engineering/Biotechnology ; Environmental factors ; Fjords ; Glaciers ; Glaciohydrology ; Ice environments ; Life Sciences ; Microbiology ; Microorganisms ; NifH gene ; Nitrogen ; Nitrogen fixation ; Nitrogenation ; Organic carbon ; Organic matter ; Original Paper ; Runoff ; Sediments ; Sequence analysis ; Sulfur ; Total organic carbon</subject><ispartof>World journal of microbiology & biotechnology, 2021-03, Vol.37 (3), p.41-41, Article 41</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-2e0c30bbbaf62aeb43f27ee0c66448ce75cf94861a0611be161068f3ca879f003</citedby><cites>FETCH-LOGICAL-c478t-2e0c30bbbaf62aeb43f27ee0c66448ce75cf94861a0611be161068f3ca879f003</cites><orcidid>0000-0003-1101-657X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-020-02993-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-020-02993-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33544264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jabir, T.</creatorcontrib><creatorcontrib>Vipindas, P. V.</creatorcontrib><creatorcontrib>Krishnan, K. P.</creatorcontrib><creatorcontrib>Mohamed Hatha, A. A.</creatorcontrib><title>Abundance and diversity of diazotrophs in the surface sediments of Kongsfjorden, an Arctic fjord</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Diazotrophy in the Arctic environment is poorly understood compared to tropical and subtropical regions. Hence in this study, we report the abundance and diversity of diazotrophs in Arctic fjord sediments and elucidate the role of environmental factors on the distribution of diazotrophs. The study was conducted during the boreal summer in the Kongsfjorden, an Arctic fjord situated in the western coast of Spitsbergen. The abundance of
nifH
gene was measured through quantitative real-time PCR and the diversity of diazotrophs was assessed by
nifH
targeted clone library and next generation sequence analysis. Results revealed that the abundance of
nifH
gene in the surface sediments ranged from 2.3 × 10
6
to 3.7 × 10
7
copies g
− 1
. The δ-proteobacterial diazotrophs (71% of total sequence) were the dominant class observed in this study. Major genera retrieved from the sequence analysis were Desulfovibrionaceae (25% of total sequence), Desulfuromonadaceae (18% of total sequence) and Desulfobacteriaceae (10% of total sequence); these are important diazotrophic iron and sulfur-reducing bacterial clade in the Kongsfjorden sediments. The abundance of
nifH
gene showed a significant positive correlation TOC/TN ratio (r
2
= 0.96, p ≤ 0.05) and total organic carbon (p ≤ 0.05) content in the fjord sediments. The higher TOC/TN ratio (4.24–14.5) indicated low nitrogen content organic matter in the fjord sediments through glacier runoff, which enhances the abundance and diversity of nitrogen fixing microorganisms.
Graphic abstract</description><subject>Abundance</subject><subject>Applied Microbiology</subject><subject>Arctic environments</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Environmental factors</subject><subject>Fjords</subject><subject>Glaciers</subject><subject>Glaciohydrology</subject><subject>Ice environments</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>NifH gene</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Organic carbon</subject><subject>Organic matter</subject><subject>Original Paper</subject><subject>Runoff</subject><subject>Sediments</subject><subject>Sequence analysis</subject><subject>Sulfur</subject><subject>Total organic carbon</subject><issn>0959-3993</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1PGzEQhi0EKmnaP8ABrcSlhy4df6y9e4yiQlGRuLRn1-sdh40SO9i7SOHX1_lokThwGFkz87zvWC8hFxSuKYD6lihlSpTAIFfT8JKekAmtFC-hUeyUTKCpmpLnzTn5mNISIMsa_oGcc14JwaSYkD-zdvSd8RYL47ui658xpn7YFsHlxryEIYbNYyp6XwyPWKQxOpPZhF2_Rj-kHfcz-EVyyxA79F-zTTGLduhtsR99ImfOrBJ-Pr5T8vvm-6_5j_L-4fZuPrsvrVD1UDIEy6FtW-MkM9gK7pjCPJRSiNqiqqxrRC2pAUlpi1RSkLXj1tSqcQB8Sr4cfDcxPI2YBr3uk8XVyngMY9JM1IpKYGqHXr1Bl2GMPv9uR0nJgFVVptiBsjGkFNHpTezXJm41Bb3LXx_y1zl_vc9f0yy6PFqP7Rq7_5J_gWeAH4CUV36B8fX2O7Z_AY_wkAw</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Jabir, T.</creator><creator>Vipindas, P. 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V. ; Krishnan, K. P. ; Mohamed Hatha, A. 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V.</au><au>Krishnan, K. P.</au><au>Mohamed Hatha, A. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abundance and diversity of diazotrophs in the surface sediments of Kongsfjorden, an Arctic fjord</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>37</volume><issue>3</issue><spage>41</spage><epage>41</epage><pages>41-41</pages><artnum>41</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Diazotrophy in the Arctic environment is poorly understood compared to tropical and subtropical regions. Hence in this study, we report the abundance and diversity of diazotrophs in Arctic fjord sediments and elucidate the role of environmental factors on the distribution of diazotrophs. The study was conducted during the boreal summer in the Kongsfjorden, an Arctic fjord situated in the western coast of Spitsbergen. The abundance of
nifH
gene was measured through quantitative real-time PCR and the diversity of diazotrophs was assessed by
nifH
targeted clone library and next generation sequence analysis. Results revealed that the abundance of
nifH
gene in the surface sediments ranged from 2.3 × 10
6
to 3.7 × 10
7
copies g
− 1
. The δ-proteobacterial diazotrophs (71% of total sequence) were the dominant class observed in this study. Major genera retrieved from the sequence analysis were Desulfovibrionaceae (25% of total sequence), Desulfuromonadaceae (18% of total sequence) and Desulfobacteriaceae (10% of total sequence); these are important diazotrophic iron and sulfur-reducing bacterial clade in the Kongsfjorden sediments. The abundance of
nifH
gene showed a significant positive correlation TOC/TN ratio (r
2
= 0.96, p ≤ 0.05) and total organic carbon (p ≤ 0.05) content in the fjord sediments. The higher TOC/TN ratio (4.24–14.5) indicated low nitrogen content organic matter in the fjord sediments through glacier runoff, which enhances the abundance and diversity of nitrogen fixing microorganisms.
Graphic abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33544264</pmid><doi>10.1007/s11274-020-02993-1</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1101-657X</orcidid></addata></record> |
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| ispartof | World journal of microbiology & biotechnology, 2021-03, Vol.37 (3), p.41-41, Article 41 |
| issn | 0959-3993 1573-0972 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Abundance Applied Microbiology Arctic environments Biochemistry Biomedical and Life Sciences Biotechnology Environmental Engineering/Biotechnology Environmental factors Fjords Glaciers Glaciohydrology Ice environments Life Sciences Microbiology Microorganisms NifH gene Nitrogen Nitrogen fixation Nitrogenation Organic carbon Organic matter Original Paper Runoff Sediments Sequence analysis Sulfur Total organic carbon |
| title | Abundance and diversity of diazotrophs in the surface sediments of Kongsfjorden, an Arctic fjord |
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