The effect of estrogen‐like compound on rotator cuff tendon healing in a murine model
Estrogen deficiency has been shown to negatively influence rotator cuff tendon healing. Therefore, the addition of an estrogen‐like‐compound (ELC) in a nonestrogen‐deficient animal may improve the quality of a rotator cuff repair. The purpose of this study was to evaluate the effects of an ELC, diet...
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| Veröffentlicht in: | Journal of orthopaedic research 2021-12, Vol.39 (12), p.2711-2724 |
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| container_title | Journal of orthopaedic research |
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| creator | Tashjian, Robert Z. Kazmers, Nikolas H. Epperson, Richard T. Honeggar, Matthew Ma, Ying Chalmers, Peter N. Williams, Dustin L. Jurynec, Michael J. |
| description | Estrogen deficiency has been shown to negatively influence rotator cuff tendon healing. Therefore, the addition of an estrogen‐like‐compound (ELC) in a nonestrogen‐deficient animal may improve the quality of a rotator cuff repair. The purpose of this study was to evaluate the effects of an ELC, diethylstilbestrol (DES), on tendon healing in a murine rotator cuff repair model. Thirty‐three male wild‐type mice (C57BL/6NJ) were randomly divided into three study groups. Group 1—unoperated mice with normal rotator cuff tendons. Groups 2 and 3 consisted of surgically repaired rotator cuff tendons; Group 2 (repair‐only) was the standard repair group (no DES injected), whereas Group 3 (repair + DES) was the experimental repair group (injected with DES). Comparing the maximal thickness of calcified fibrocartilage to uncalcified fibrocartilage, the ratios for the control (intact tendon), repair‐only, and repair + DES groups were 2:1, 0.9:1, and 1.7:1. RNA expression data demonstrated upregulation of chondrogenic, angiogenic, and tendon modulation genes in the repair‐ only group compared to the control (intact tendon) group (p |
| doi_str_mv | 10.1002/jor.25000 |
| format | Article |
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Therefore, the addition of an estrogen‐like‐compound (ELC) in a nonestrogen‐deficient animal may improve the quality of a rotator cuff repair. The purpose of this study was to evaluate the effects of an ELC, diethylstilbestrol (DES), on tendon healing in a murine rotator cuff repair model. Thirty‐three male wild‐type mice (C57BL/6NJ) were randomly divided into three study groups. Group 1—unoperated mice with normal rotator cuff tendons. Groups 2 and 3 consisted of surgically repaired rotator cuff tendons; Group 2 (repair‐only) was the standard repair group (no DES injected), whereas Group 3 (repair + DES) was the experimental repair group (injected with DES). Comparing the maximal thickness of calcified fibrocartilage to uncalcified fibrocartilage, the ratios for the control (intact tendon), repair‐only, and repair + DES groups were 2:1, 0.9:1, and 1.7:1. RNA expression data demonstrated upregulation of chondrogenic, angiogenic, and tendon modulation genes in the repair‐ only group compared to the control (intact tendon) group (p < 0.04 for all), and that addition of DES further increased the osteogenic, angiogenic, and tendon modulation gene expression compared to the repair‐only group (p < 0.02). Immunohistochemical analysis indicated that the addition of DES further increased osteogenic, angiogenic, and tendon maturation protein expression at the enthesis compared to standard repairs.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.25000</identifier><identifier>PMID: 33533088</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Biomechanical Phenomena ; Disease Models, Animal ; estrogen ; Estrogens - pharmacology ; Estrogens - therapeutic use ; estrogen‐like compound ; histology ; Male ; Mice ; Mice, Inbred C57BL ; mouse model ; RNA expression ; rotator cuff ; Rotator Cuff - surgery ; Rotator Cuff Injuries - drug therapy ; Rotator Cuff Injuries - surgery ; scanning electron microscopy ; Tendons - surgery ; Wound Healing</subject><ispartof>Journal of orthopaedic research, 2021-12, Vol.39 (12), p.2711-2724</ispartof><rights>2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC</rights><rights>2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3600-2549ca15909d492879778d25c51598edab9323e7ed428757309df56719d3bfaa3</citedby><cites>FETCH-LOGICAL-c3600-2549ca15909d492879778d25c51598edab9323e7ed428757309df56719d3bfaa3</cites><orcidid>0000-0003-4112-0423 ; 0000-0003-2702-9164 ; 0000-0001-6736-8641</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjor.25000$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjor.25000$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33533088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tashjian, Robert Z.</creatorcontrib><creatorcontrib>Kazmers, Nikolas H.</creatorcontrib><creatorcontrib>Epperson, Richard T.</creatorcontrib><creatorcontrib>Honeggar, Matthew</creatorcontrib><creatorcontrib>Ma, Ying</creatorcontrib><creatorcontrib>Chalmers, Peter N.</creatorcontrib><creatorcontrib>Williams, Dustin L.</creatorcontrib><creatorcontrib>Jurynec, Michael J.</creatorcontrib><title>The effect of estrogen‐like compound on rotator cuff tendon healing in a murine model</title><title>Journal of orthopaedic research</title><addtitle>J Orthop Res</addtitle><description>Estrogen deficiency has been shown to negatively influence rotator cuff tendon healing. Therefore, the addition of an estrogen‐like‐compound (ELC) in a nonestrogen‐deficient animal may improve the quality of a rotator cuff repair. The purpose of this study was to evaluate the effects of an ELC, diethylstilbestrol (DES), on tendon healing in a murine rotator cuff repair model. Thirty‐three male wild‐type mice (C57BL/6NJ) were randomly divided into three study groups. Group 1—unoperated mice with normal rotator cuff tendons. Groups 2 and 3 consisted of surgically repaired rotator cuff tendons; Group 2 (repair‐only) was the standard repair group (no DES injected), whereas Group 3 (repair + DES) was the experimental repair group (injected with DES). Comparing the maximal thickness of calcified fibrocartilage to uncalcified fibrocartilage, the ratios for the control (intact tendon), repair‐only, and repair + DES groups were 2:1, 0.9:1, and 1.7:1. RNA expression data demonstrated upregulation of chondrogenic, angiogenic, and tendon modulation genes in the repair‐ only group compared to the control (intact tendon) group (p < 0.04 for all), and that addition of DES further increased the osteogenic, angiogenic, and tendon modulation gene expression compared to the repair‐only group (p < 0.02). Immunohistochemical analysis indicated that the addition of DES further increased osteogenic, angiogenic, and tendon maturation protein expression at the enthesis compared to standard repairs.</description><subject>Animals</subject><subject>Biomechanical Phenomena</subject><subject>Disease Models, Animal</subject><subject>estrogen</subject><subject>Estrogens - pharmacology</subject><subject>Estrogens - therapeutic use</subject><subject>estrogen‐like compound</subject><subject>histology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>mouse model</subject><subject>RNA expression</subject><subject>rotator cuff</subject><subject>Rotator Cuff - surgery</subject><subject>Rotator Cuff Injuries - drug therapy</subject><subject>Rotator Cuff Injuries - surgery</subject><subject>scanning electron microscopy</subject><subject>Tendons - surgery</subject><subject>Wound Healing</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtOwzAQQC0EoqWw4ALIS1ik9SeOkyWq-KpSJVQEu8iNx21KEhc7EeqOI3BGToKhhR2rkWaenkYPoVNKhpQQNlpZN2SCELKH-lSIOBJMPu-jPpE8iQhLkh468n4VAElZeoh6nAvOSZr20dNsCRiMgaLF1mDwrbMLaD7fP6ryBXBh67XtGo1tg51tVWsdLjpjcAuNDrslqKpsFrhssMJ158oGcG01VMfowKjKw8luDtDj9dVsfBtNpjd348tJVPCEkIiJOCsUFRnJdJyxVGZSppqJQoRdClrNM844SNBxOArJA2dEImmm-dwoxQfofOtdO_vahffzuvQFVJVqwHY-Z3Ga0ITzlAf0YosWznrvwORrV9bKbXJK8u-OeeiY_3QM7NlO281r0H_kb7gAjLbAW1nB5n9Tfj992Cq_ALDHfGs</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Tashjian, Robert Z.</creator><creator>Kazmers, Nikolas H.</creator><creator>Epperson, Richard T.</creator><creator>Honeggar, Matthew</creator><creator>Ma, Ying</creator><creator>Chalmers, Peter N.</creator><creator>Williams, Dustin L.</creator><creator>Jurynec, Michael J.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4112-0423</orcidid><orcidid>https://orcid.org/0000-0003-2702-9164</orcidid><orcidid>https://orcid.org/0000-0001-6736-8641</orcidid></search><sort><creationdate>202112</creationdate><title>The effect of estrogen‐like compound on rotator cuff tendon healing in a murine model</title><author>Tashjian, Robert Z. ; Kazmers, Nikolas H. ; Epperson, Richard T. ; Honeggar, Matthew ; Ma, Ying ; Chalmers, Peter N. ; Williams, Dustin L. ; Jurynec, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3600-2549ca15909d492879778d25c51598edab9323e7ed428757309df56719d3bfaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biomechanical Phenomena</topic><topic>Disease Models, Animal</topic><topic>estrogen</topic><topic>Estrogens - pharmacology</topic><topic>Estrogens - therapeutic use</topic><topic>estrogen‐like compound</topic><topic>histology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>mouse model</topic><topic>RNA expression</topic><topic>rotator cuff</topic><topic>Rotator Cuff - surgery</topic><topic>Rotator Cuff Injuries - drug therapy</topic><topic>Rotator Cuff Injuries - surgery</topic><topic>scanning electron microscopy</topic><topic>Tendons - surgery</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tashjian, Robert Z.</creatorcontrib><creatorcontrib>Kazmers, Nikolas H.</creatorcontrib><creatorcontrib>Epperson, Richard T.</creatorcontrib><creatorcontrib>Honeggar, Matthew</creatorcontrib><creatorcontrib>Ma, Ying</creatorcontrib><creatorcontrib>Chalmers, Peter N.</creatorcontrib><creatorcontrib>Williams, Dustin L.</creatorcontrib><creatorcontrib>Jurynec, Michael J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tashjian, Robert Z.</au><au>Kazmers, Nikolas H.</au><au>Epperson, Richard T.</au><au>Honeggar, Matthew</au><au>Ma, Ying</au><au>Chalmers, Peter N.</au><au>Williams, Dustin L.</au><au>Jurynec, Michael J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of estrogen‐like compound on rotator cuff tendon healing in a murine model</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J Orthop Res</addtitle><date>2021-12</date><risdate>2021</risdate><volume>39</volume><issue>12</issue><spage>2711</spage><epage>2724</epage><pages>2711-2724</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>Estrogen deficiency has been shown to negatively influence rotator cuff tendon healing. Therefore, the addition of an estrogen‐like‐compound (ELC) in a nonestrogen‐deficient animal may improve the quality of a rotator cuff repair. The purpose of this study was to evaluate the effects of an ELC, diethylstilbestrol (DES), on tendon healing in a murine rotator cuff repair model. Thirty‐three male wild‐type mice (C57BL/6NJ) were randomly divided into three study groups. Group 1—unoperated mice with normal rotator cuff tendons. Groups 2 and 3 consisted of surgically repaired rotator cuff tendons; Group 2 (repair‐only) was the standard repair group (no DES injected), whereas Group 3 (repair + DES) was the experimental repair group (injected with DES). Comparing the maximal thickness of calcified fibrocartilage to uncalcified fibrocartilage, the ratios for the control (intact tendon), repair‐only, and repair + DES groups were 2:1, 0.9:1, and 1.7:1. RNA expression data demonstrated upregulation of chondrogenic, angiogenic, and tendon modulation genes in the repair‐ only group compared to the control (intact tendon) group (p < 0.04 for all), and that addition of DES further increased the osteogenic, angiogenic, and tendon modulation gene expression compared to the repair‐only group (p < 0.02). Immunohistochemical analysis indicated that the addition of DES further increased osteogenic, angiogenic, and tendon maturation protein expression at the enthesis compared to standard repairs.</abstract><cop>United States</cop><pmid>33533088</pmid><doi>10.1002/jor.25000</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-4112-0423</orcidid><orcidid>https://orcid.org/0000-0003-2702-9164</orcidid><orcidid>https://orcid.org/0000-0001-6736-8641</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biomechanical Phenomena Disease Models, Animal estrogen Estrogens - pharmacology Estrogens - therapeutic use estrogen‐like compound histology Male Mice Mice, Inbred C57BL mouse model RNA expression rotator cuff Rotator Cuff - surgery Rotator Cuff Injuries - drug therapy Rotator Cuff Injuries - surgery scanning electron microscopy Tendons - surgery Wound Healing |
| title | The effect of estrogen‐like compound on rotator cuff tendon healing in a murine model |
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