Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes
This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 1...
Gespeichert in:
| Veröffentlicht in: | Analytical Sciences 2021/08/10, Vol.37(8), pp.1157-1163 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1163 |
|---|---|
| container_issue | 8 |
| container_start_page | 1157 |
| container_title | Analytical Sciences |
| container_volume | 37 |
| creator | Claudio A. G. A. VIEIRA PUPIN, Breno BHATTACHARJEE, Tanmoy T. SAKANE, Kumiko K. |
| description | This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 – 20 km/h) speeds, and PCA–linear-discriminant-analysis showed 100% classification of 18 – 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests. |
| doi_str_mv | 10.2116/analsci.20P395 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2484246428</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2484246428</sourcerecordid><originalsourceid>FETCH-LOGICAL-c682t-7a4ba0f7e55a6d88c68de34c649e17fe15bf94caced2c73fee71a8f3b8b7df093</originalsourceid><addsrcrecordid>eNp1kc1v1DAQxS0EokvhyhFZ4sIlW38lcY7t8lWpiEotZ2vijHe9yibBTir2v8dRlkVC4mJL49-8eX5DyFvO1oLz4go6aKP1a8HuZZU_Iysulc6EUMVzsmIVZ1khFbsgr2LcM8aFFuIluZAy5zrXakW6284FCNjQhwHtGPpo--FIbyDOpXFqjrR39Mb3docHb6Glmx10W4zUd_QBWv8EtJmC77b0G_zyhwTch34bMEb_hPQR4ziT1-OuxRHja_LCJcP45nRfkh-fPz1uvmZ337_cbq7vMltoMWYlqBqYKzHPoWi0TtUGpbKFqpCXDnleu0pZsNgIW0qHWHLQTta6LhvHKnlJPiy6Q-h_TsmEOfhosW2hw36KRiitUkZK6IS-_wfd91OYYzUiT26qSuQiUeuFsimiGNCZIaTfhqPhzMybMKdNmGUTqeHdSXaqD9ic8T_RJ-BqAeIwx4fh79z_Sn5cOvZxhC2eJSGM3rZ4xmVp9Hwsbednu4NgsJO_AROLsBI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2568299252</pqid></control><display><type>article</type><title>Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Freely Accessible Japanese Titles</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><source>SpringerLink Journals - AutoHoldings</source><creator>Claudio A. G. A. VIEIRA ; PUPIN, Breno ; BHATTACHARJEE, Tanmoy T. ; SAKANE, Kumiko K.</creator><creatorcontrib>Claudio A. G. A. VIEIRA ; PUPIN, Breno ; BHATTACHARJEE, Tanmoy T. ; SAKANE, Kumiko K.</creatorcontrib><description>This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 – 20 km/h) speeds, and PCA–linear-discriminant-analysis showed 100% classification of 18 – 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.20P395</identifier><identifier>PMID: 33518584</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Athletes ; ATR-FTIR ; Biochemistry ; Blood ; Carbohydrates ; Chemistry ; Discriminant Analysis ; exercise associated biochemical changes ; exercise related changes in saliva ; Fourier transforms ; Glucose ; Humans ; Infrared spectroscopy ; Lactic acid ; Lipids ; Maximal stress test ; Multivariate analysis ; Original Paper ; PCA-LDA ; Physical training ; Principal Component Analysis ; Saliva ; Spectroscopy, Fourier Transform Infrared ; Spectrum analysis ; Treadmills</subject><ispartof>Analytical Sciences, 2021/08/10, Vol.37(8), pp.1157-1163</ispartof><rights>2021 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2021</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c682t-7a4ba0f7e55a6d88c68de34c649e17fe15bf94caced2c73fee71a8f3b8b7df093</citedby><cites>FETCH-LOGICAL-c682t-7a4ba0f7e55a6d88c68de34c649e17fe15bf94caced2c73fee71a8f3b8b7df093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.20P395$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.20P395$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,1877,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33518584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Claudio A. G. A. VIEIRA</creatorcontrib><creatorcontrib>PUPIN, Breno</creatorcontrib><creatorcontrib>BHATTACHARJEE, Tanmoy T.</creatorcontrib><creatorcontrib>SAKANE, Kumiko K.</creatorcontrib><title>Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 – 20 km/h) speeds, and PCA–linear-discriminant-analysis showed 100% classification of 18 – 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests.</description><subject>Analytical Chemistry</subject><subject>Athletes</subject><subject>ATR-FTIR</subject><subject>Biochemistry</subject><subject>Blood</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Discriminant Analysis</subject><subject>exercise associated biochemical changes</subject><subject>exercise related changes in saliva</subject><subject>Fourier transforms</subject><subject>Glucose</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Lactic acid</subject><subject>Lipids</subject><subject>Maximal stress test</subject><subject>Multivariate analysis</subject><subject>Original Paper</subject><subject>PCA-LDA</subject><subject>Physical training</subject><subject>Principal Component Analysis</subject><subject>Saliva</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Spectrum analysis</subject><subject>Treadmills</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1v1DAQxS0EokvhyhFZ4sIlW38lcY7t8lWpiEotZ2vijHe9yibBTir2v8dRlkVC4mJL49-8eX5DyFvO1oLz4go6aKP1a8HuZZU_Iysulc6EUMVzsmIVZ1khFbsgr2LcM8aFFuIluZAy5zrXakW6284FCNjQhwHtGPpo--FIbyDOpXFqjrR39Mb3docHb6Glmx10W4zUd_QBWv8EtJmC77b0G_zyhwTch34bMEb_hPQR4ziT1-OuxRHja_LCJcP45nRfkh-fPz1uvmZ337_cbq7vMltoMWYlqBqYKzHPoWi0TtUGpbKFqpCXDnleu0pZsNgIW0qHWHLQTta6LhvHKnlJPiy6Q-h_TsmEOfhosW2hw36KRiitUkZK6IS-_wfd91OYYzUiT26qSuQiUeuFsimiGNCZIaTfhqPhzMybMKdNmGUTqeHdSXaqD9ic8T_RJ-BqAeIwx4fh79z_Sn5cOvZxhC2eJSGM3rZ4xmVp9Hwsbednu4NgsJO_AROLsBI</recordid><startdate>20210810</startdate><enddate>20210810</enddate><creator>Claudio A. G. A. VIEIRA</creator><creator>PUPIN, Breno</creator><creator>BHATTACHARJEE, Tanmoy T.</creator><creator>SAKANE, Kumiko K.</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210810</creationdate><title>Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes</title><author>Claudio A. G. A. VIEIRA ; PUPIN, Breno ; BHATTACHARJEE, Tanmoy T. ; SAKANE, Kumiko K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c682t-7a4ba0f7e55a6d88c68de34c649e17fe15bf94caced2c73fee71a8f3b8b7df093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Athletes</topic><topic>ATR-FTIR</topic><topic>Biochemistry</topic><topic>Blood</topic><topic>Carbohydrates</topic><topic>Chemistry</topic><topic>Discriminant Analysis</topic><topic>exercise associated biochemical changes</topic><topic>exercise related changes in saliva</topic><topic>Fourier transforms</topic><topic>Glucose</topic><topic>Humans</topic><topic>Infrared spectroscopy</topic><topic>Lactic acid</topic><topic>Lipids</topic><topic>Maximal stress test</topic><topic>Multivariate analysis</topic><topic>Original Paper</topic><topic>PCA-LDA</topic><topic>Physical training</topic><topic>Principal Component Analysis</topic><topic>Saliva</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Spectrum analysis</topic><topic>Treadmills</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Claudio A. G. A. VIEIRA</creatorcontrib><creatorcontrib>PUPIN, Breno</creatorcontrib><creatorcontrib>BHATTACHARJEE, Tanmoy T.</creatorcontrib><creatorcontrib>SAKANE, Kumiko K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Claudio A. G. A. VIEIRA</au><au>PUPIN, Breno</au><au>BHATTACHARJEE, Tanmoy T.</au><au>SAKANE, Kumiko K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. SCI</stitle><addtitle>Anal Sci</addtitle><date>2021-08-10</date><risdate>2021</risdate><volume>37</volume><issue>8</issue><spage>1157</spage><epage>1163</epage><pages>1157-1163</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>This study aims to explore biochemical changes in saliva during cardiorespiratory exercise using attenuated-total-reflectance–Fourier-transform-infrared-spectroscopy (ATR-FTIR). Saliva and blood samples were obtained from six athletes at rest, and after running at speeds of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 20 kilometers-per-hour (km/h) on a treadmill (maximal stress test). Saliva ATR-FTIR spectra were analyzed using deconvolution and multivariate analysis. Area-under-the-curve calculations suggest differential changes in glucose, lactate, protein, lipids, carbohydrate and phosphate content in saliva during the test. Increases in glucose and lactate levels with increasing speeds were verified by simultaneous measurement of blood glucose and lactate levels using standard equipment (Roche®). Multivariate principal-component-analysis (PCA) showed discrete clusters for low (rest-14 km/h) and high (15 – 20 km/h) speeds, and PCA–linear-discriminant-analysis showed 100% classification of 18 – 20 km/h as high speed. Overall, results suggest the possibility of using this non-invasive saliva-based ATR-FTIR method for biochemical assessment during sports exercise and stress tests.</abstract><cop>Singapore</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>33518584</pmid><doi>10.2116/analsci.20P395</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0910-6340 |
| ispartof | Analytical Sciences, 2021/08/10, Vol.37(8), pp.1157-1163 |
| issn | 0910-6340 1348-2246 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2484246428 |
| source | J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Athletes ATR-FTIR Biochemistry Blood Carbohydrates Chemistry Discriminant Analysis exercise associated biochemical changes exercise related changes in saliva Fourier transforms Glucose Humans Infrared spectroscopy Lactic acid Lipids Maximal stress test Multivariate analysis Original Paper PCA-LDA Physical training Principal Component Analysis Saliva Spectroscopy, Fourier Transform Infrared Spectrum analysis Treadmills |
| title | Infrared Spectroscopy Based Study of Biochemical Changes in Saliva during Maximal Progressive Test in Athletes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T12%3A15%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Infrared%20Spectroscopy%20Based%20Study%20of%20Biochemical%20Changes%20in%20Saliva%20during%20Maximal%20Progressive%20Test%20in%20Athletes&rft.jtitle=Analytical%20Sciences&rft.au=Claudio%20A.%20G.%20A.%20VIEIRA&rft.date=2021-08-10&rft.volume=37&rft.issue=8&rft.spage=1157&rft.epage=1163&rft.pages=1157-1163&rft.issn=0910-6340&rft.eissn=1348-2246&rft_id=info:doi/10.2116/analsci.20P395&rft_dat=%3Cproquest_cross%3E2484246428%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2568299252&rft_id=info:pmid/33518584&rfr_iscdi=true |