Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales
Objectives: To evaluate the biochemical Rapid ESBL NP ® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales. Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers wi...
Gespeichert in:
| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2021-08, Vol.27 (8), p.1131-1135 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1135 |
|---|---|
| container_issue | 8 |
| container_start_page | 1131 |
| container_title | Microbial drug resistance (Larchmont, N.Y.) |
| container_volume | 27 |
| creator | Demord, Anthony Poirel, Laurent D'Emidio, Fernando Pomponio, Stefano Nordmann, Patrice |
| description | Objectives:
To evaluate the biochemical Rapid ESBL NP
®
(Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.
Methods:
A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.
Results:
The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.
Conclusion:
The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand. |
| doi_str_mv | 10.1089/mdr.2020.0391 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2483421234</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2483421234</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-f4a81d2e1e23ce4db35469da19dd6d428df701563411bb39f1bf94bd5ba985ec3</originalsourceid><addsrcrecordid>eNqFkc9O3DAQxi1U1IVtj1wrS730ksV_s_GRwgKVVrACerbseCwFJXFqJxK8Vh-kz4SjBQ69cJrR55--Gc-H0AklK0oqddq5uGKEkRXhih6gI6o4LSohqk-5J-uyKJkSC3Sc0iMhRNKSf0YLzjmRqlJHyN-ZoXF4c_9zi292-AHSiH2IeC9fwAj12IQeB483T4PpHbjifshinDr872-xNfVoOpMA72JwUw0x4abHm36EGGx-hGhaSF_QoTdtgq-vdYl-X24ezq-L7e3Vr_OzbVELIsbCC1NRx4AC4zUIZ7kUpXKGKudKJ1jl_JpQWXJBqbVceWq9EtZJa1QloeZL9GPvO8TwZ8qf0V2Tamhb00OYkmai4oJRxkVGv_-HPoYp9nk7zWSZh0gmZ6rYU3UMKUXweohNZ-KzpkTPAegcgJ4D0HMAmf_26jrZDtw7_XbxDPA9MMum79sGLMTxA9sXQ5WSOQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2561565254</pqid></control><display><type>article</type><title>Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice</creator><creatorcontrib>Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice</creatorcontrib><description>Objectives:
To evaluate the biochemical Rapid ESBL NP
®
(Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.
Methods:
A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.
Results:
The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.
Conclusion:
The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2020.0391</identifier><identifier>PMID: 33305989</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Anti-Bacterial Agents - pharmacology ; Carbapenem-Resistant Enterobacteriaceae - drug effects ; Carbapenemase ; Cephalosporinase ; Colorimetry ; Colorimetry - methods ; Enterobacterales ; Enterobacteriaceae Infections - diagnosis ; Humans ; Mechanisms ; Microbial Sensitivity Tests ; Sensitivity and Specificity ; Time Factors ; β Lactamase</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2021-08, Vol.27 (8), p.1131-1135</ispartof><rights>2021, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Aug 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-f4a81d2e1e23ce4db35469da19dd6d428df701563411bb39f1bf94bd5ba985ec3</citedby><cites>FETCH-LOGICAL-c404t-f4a81d2e1e23ce4db35469da19dd6d428df701563411bb39f1bf94bd5ba985ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33305989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demord, Anthony</creatorcontrib><creatorcontrib>Poirel, Laurent</creatorcontrib><creatorcontrib>D'Emidio, Fernando</creatorcontrib><creatorcontrib>Pomponio, Stefano</creatorcontrib><creatorcontrib>Nordmann, Patrice</creatorcontrib><title>Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>Objectives:
To evaluate the biochemical Rapid ESBL NP
®
(Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.
Methods:
A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.
Results:
The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.
Conclusion:
The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Carbapenem-Resistant Enterobacteriaceae - drug effects</subject><subject>Carbapenemase</subject><subject>Cephalosporinase</subject><subject>Colorimetry</subject><subject>Colorimetry - methods</subject><subject>Enterobacterales</subject><subject>Enterobacteriaceae Infections - diagnosis</subject><subject>Humans</subject><subject>Mechanisms</subject><subject>Microbial Sensitivity Tests</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>β Lactamase</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9O3DAQxi1U1IVtj1wrS730ksV_s_GRwgKVVrACerbseCwFJXFqJxK8Vh-kz4SjBQ69cJrR55--Gc-H0AklK0oqddq5uGKEkRXhih6gI6o4LSohqk-5J-uyKJkSC3Sc0iMhRNKSf0YLzjmRqlJHyN-ZoXF4c_9zi292-AHSiH2IeC9fwAj12IQeB483T4PpHbjifshinDr872-xNfVoOpMA72JwUw0x4abHm36EGGx-hGhaSF_QoTdtgq-vdYl-X24ezq-L7e3Vr_OzbVELIsbCC1NRx4AC4zUIZ7kUpXKGKudKJ1jl_JpQWXJBqbVceWq9EtZJa1QloeZL9GPvO8TwZ8qf0V2Tamhb00OYkmai4oJRxkVGv_-HPoYp9nk7zWSZh0gmZ6rYU3UMKUXweohNZ-KzpkTPAegcgJ4D0HMAmf_26jrZDtw7_XbxDPA9MMum79sGLMTxA9sXQ5WSOQ</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Demord, Anthony</creator><creator>Poirel, Laurent</creator><creator>D'Emidio, Fernando</creator><creator>Pomponio, Stefano</creator><creator>Nordmann, Patrice</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210801</creationdate><title>Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales</title><author>Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-f4a81d2e1e23ce4db35469da19dd6d428df701563411bb39f1bf94bd5ba985ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Carbapenem-Resistant Enterobacteriaceae - drug effects</topic><topic>Carbapenemase</topic><topic>Cephalosporinase</topic><topic>Colorimetry</topic><topic>Colorimetry - methods</topic><topic>Enterobacterales</topic><topic>Enterobacteriaceae Infections - diagnosis</topic><topic>Humans</topic><topic>Mechanisms</topic><topic>Microbial Sensitivity Tests</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demord, Anthony</creatorcontrib><creatorcontrib>Poirel, Laurent</creatorcontrib><creatorcontrib>D'Emidio, Fernando</creatorcontrib><creatorcontrib>Pomponio, Stefano</creatorcontrib><creatorcontrib>Nordmann, Patrice</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demord, Anthony</au><au>Poirel, Laurent</au><au>D'Emidio, Fernando</au><au>Pomponio, Stefano</au><au>Nordmann, Patrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2021-08-01</date><risdate>2021</risdate><volume>27</volume><issue>8</issue><spage>1131</spage><epage>1135</epage><pages>1131-1135</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>Objectives:
To evaluate the biochemical Rapid ESBL NP
®
(Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.
Methods:
A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique.
Results:
The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40–45 minutes), easy to perform, and reliable for identification of ESBL producers.
Conclusion:
The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>33305989</pmid><doi>10.1089/mdr.2020.0391</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2021-08, Vol.27 (8), p.1131-1135 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2483421234 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - pharmacology Carbapenem-Resistant Enterobacteriaceae - drug effects Carbapenemase Cephalosporinase Colorimetry Colorimetry - methods Enterobacterales Enterobacteriaceae Infections - diagnosis Humans Mechanisms Microbial Sensitivity Tests Sensitivity and Specificity Time Factors β Lactamase |
| title | Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T01%3A05%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20ESBL%20NP%20Test%20for%20Rapid%20Detection%20of%20Expanded-Spectrum%20%CE%B2-Lactamase%20Producers%20in%20Enterobacterales&rft.jtitle=Microbial%20drug%20resistance%20(Larchmont,%20N.Y.)&rft.au=Demord,%20Anthony&rft.date=2021-08-01&rft.volume=27&rft.issue=8&rft.spage=1131&rft.epage=1135&rft.pages=1131-1135&rft.issn=1076-6294&rft.eissn=1931-8448&rft_id=info:doi/10.1089/mdr.2020.0391&rft_dat=%3Cproquest_cross%3E2483421234%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2561565254&rft_id=info:pmid/33305989&rfr_iscdi=true |