Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment
A novel sulfur-oxidizing bacterium, designated strain LSR1 , was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2021-02, Vol.71 (2) |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Xie, Zhangzhang Li, Surong Lin, Weitie Luo, Jianfei |
| description | A novel sulfur-oxidizing bacterium, designated strain LSR1
, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1
was observed at 15-40 °C, pH 6.0-7.5 and NaCl concentrations of 0-1.5 %. Strain LSR1
was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C
and summed feature 3 (comprising C
ω7
and/or C
ω6
). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1
formed a lineage within the family
, showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera
,
and
, respectively. The genome of strain LSR1
contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding
-type cytochrome c oxidase and
-type quinol oxidase, which enables strain LSR1
to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1
is considered to represent a novel species of a new genus
within the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain is LSR1
(=GDMCC 1.1549=JCM 33645). |
| doi_str_mv | 10.1099/ijsem.0.004657 |
| format | Article |
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, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1
was observed at 15-40 °C, pH 6.0-7.5 and NaCl concentrations of 0-1.5 %. Strain LSR1
was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C
and summed feature 3 (comprising C
ω7
and/or C
ω6
). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1
formed a lineage within the family
, showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera
,
and
, respectively. The genome of strain LSR1
contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding
-type cytochrome c oxidase and
-type quinol oxidase, which enables strain LSR1
to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1
is considered to represent a novel species of a new genus
within the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain is LSR1
(=GDMCC 1.1549=JCM 33645).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.004657</identifier><identifier>PMID: 33502294</identifier><language>eng</language><publisher>England</publisher><subject>Bacterial Typing Techniques ; Base Composition ; Betaproteobacteria - classification ; Betaproteobacteria - isolation & purification ; China ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Fresh Water - microbiology ; Geologic Sediments - microbiology ; Oxidation-Reduction ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sulfur-Reducing Bacteria - classification ; Sulfur-Reducing Bacteria - isolation & purification</subject><ispartof>International journal of systematic and evolutionary microbiology, 2021-02, Vol.71 (2)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-cebde430d48b99528f87db0310fcbeaddb2ee59ec14b94f0cf3e50138d0eaf453</citedby><cites>FETCH-LOGICAL-c335t-cebde430d48b99528f87db0310fcbeaddb2ee59ec14b94f0cf3e50138d0eaf453</cites><orcidid>0000-0003-0833-0605 ; 0000-0002-9570-4224</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3732,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33502294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Zhangzhang</creatorcontrib><creatorcontrib>Li, Surong</creatorcontrib><creatorcontrib>Lin, Weitie</creatorcontrib><creatorcontrib>Luo, Jianfei</creatorcontrib><title>Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A novel sulfur-oxidizing bacterium, designated strain LSR1
, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1
was observed at 15-40 °C, pH 6.0-7.5 and NaCl concentrations of 0-1.5 %. Strain LSR1
was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C
and summed feature 3 (comprising C
ω7
and/or C
ω6
). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1
formed a lineage within the family
, showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera
,
and
, respectively. The genome of strain LSR1
contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding
-type cytochrome c oxidase and
-type quinol oxidase, which enables strain LSR1
to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1
is considered to represent a novel species of a new genus
within the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain is LSR1
(=GDMCC 1.1549=JCM 33645).</description><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Betaproteobacteria - classification</subject><subject>Betaproteobacteria - isolation & purification</subject><subject>China</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Fresh Water - microbiology</subject><subject>Geologic Sediments - microbiology</subject><subject>Oxidation-Reduction</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sulfur-Reducing Bacteria - classification</subject><subject>Sulfur-Reducing Bacteria - isolation & purification</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9ULtOxDAQtBCIx0FLiVxSkLCOnVxSIsRLOgkKqCPHXh9GSXx4E14df07ggGZ3djQ72h3GDgWkAqrq1D8RdimkAKrI5xtsV6iiSHKQavMfZ8UO2yN6ApgIgG22I-XEZpXaZZ93OmoaWzdGP4Q4jMSN7ofQY0-e-BL7lPfhJT3htPpDmnfexKAxhtWjb73ha4MkvHnrP3y_5I02A0Y_dtxTaPWAlrsYuqkgPb5Oc-SE1nfYD_tsy-mW8OC3z9jD5cX9-XWyuL26OT9bJGa6dkgMNhaVBKvKpqryrHTl3DYgBTjToLa2yRDzCo1QTaUcGCcxByFLC6idyuWMHa99VzE8j0hD3Xky2La6xzBSnalSFEUp5mKSpmvp9CVRRFevou90fK8F1N-x1z-x11CvY58Wjn69x6ZD-y__y1l-AbYggu0</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Xie, Zhangzhang</creator><creator>Li, Surong</creator><creator>Lin, Weitie</creator><creator>Luo, Jianfei</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0833-0605</orcidid><orcidid>https://orcid.org/0000-0002-9570-4224</orcidid></search><sort><creationdate>202102</creationdate><title>Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment</title><author>Xie, Zhangzhang ; Li, Surong ; Lin, Weitie ; Luo, Jianfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-cebde430d48b99528f87db0310fcbeaddb2ee59ec14b94f0cf3e50138d0eaf453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Betaproteobacteria - classification</topic><topic>Betaproteobacteria - isolation & purification</topic><topic>China</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Fresh Water - microbiology</topic><topic>Geologic Sediments - microbiology</topic><topic>Oxidation-Reduction</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sulfur-Reducing Bacteria - classification</topic><topic>Sulfur-Reducing Bacteria - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Zhangzhang</creatorcontrib><creatorcontrib>Li, Surong</creatorcontrib><creatorcontrib>Lin, Weitie</creatorcontrib><creatorcontrib>Luo, Jianfei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Zhangzhang</au><au>Li, Surong</au><au>Lin, Weitie</au><au>Luo, Jianfei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2021-02</date><risdate>2021</risdate><volume>71</volume><issue>2</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel sulfur-oxidizing bacterium, designated strain LSR1
, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1
was observed at 15-40 °C, pH 6.0-7.5 and NaCl concentrations of 0-1.5 %. Strain LSR1
was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C
and summed feature 3 (comprising C
ω7
and/or C
ω6
). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1
formed a lineage within the family
, showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera
,
and
, respectively. The genome of strain LSR1
contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding
-type cytochrome c oxidase and
-type quinol oxidase, which enables strain LSR1
to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1
is considered to represent a novel species of a new genus
within the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain is LSR1
(=GDMCC 1.1549=JCM 33645).</abstract><cop>England</cop><pmid>33502294</pmid><doi>10.1099/ijsem.0.004657</doi><orcidid>https://orcid.org/0000-0003-0833-0605</orcidid><orcidid>https://orcid.org/0000-0002-9570-4224</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Bacterial Typing Techniques Base Composition Betaproteobacteria - classification Betaproteobacteria - isolation & purification China DNA, Bacterial - genetics Fatty Acids - chemistry Fresh Water - microbiology Geologic Sediments - microbiology Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sulfur-Reducing Bacteria - classification Sulfur-Reducing Bacteria - isolation & purification |
| title | Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment |
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