An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21
A sensitive ratiometric fluorescence probe based on hybridization chain reaction (HCR) was constructed for sensitive detection of miRNA-21 by using G-triplex and silver nanocluster pairs (AgNC pairs) as an enzyme-free and label-free signal output group. miRNA-21 was used as the primer for the hybrid...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2021-02, Vol.188 (2), p.55-55, Article 55 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Zhao, Xiaojia Wang, Shuang Zou, Rong Chen, Chunyan Cai, Changqun |
| description | A sensitive ratiometric fluorescence probe based on hybridization chain reaction (HCR) was constructed for sensitive detection of miRNA-21 by using G-triplex and silver nanocluster pairs (AgNC pairs) as an enzyme-free and label-free signal output group. miRNA-21 was used as the primer for the hybridization chain reaction of molecular beacon 1 (MB1) containing the locked G-triplex sequence and molecular beacon 2 (MB2) with intact AgNC pairs at the 5′ and 3′ end activation. The double-stranded product was obtained along with the opening of the G-triplex and the separation of the AgNC pairs. A detection limit of 67 pM and a linear detection range of 0.1–300 nM were obtained for miRNA-21 determination. The proposed strategy enabled the monitoring of miRNA-21 levels in at least three cell lines, indicating that it provided new ideas for detecting miRNA in real samples.
Graphical abstract
MB1 and MB2 contained the locked G-triplex sequence and silver nanocluster pairs (AgNC pairs), respectively. In the presence of target, the hybridization chain reaction (HCR) between MB1 and MB2 was initiated. At the same time, the locked G-triplex was released and combined to the dye thioflavin T (THT) to increase fluorescence, while the separation of the AgNC pairs caused the fluorescence to decrease. The double-stranded (ds) DNA product was generated to form a ratiometric signal to be detected. |
| doi_str_mv | 10.1007/s00604-020-04680-2 |
| format | Article |
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Graphical abstract
MB1 and MB2 contained the locked G-triplex sequence and silver nanocluster pairs (AgNC pairs), respectively. In the presence of target, the hybridization chain reaction (HCR) between MB1 and MB2 was initiated. At the same time, the locked G-triplex was released and combined to the dye thioflavin T (THT) to increase fluorescence, while the separation of the AgNC pairs caused the fluorescence to decrease. The double-stranded (ds) DNA product was generated to form a ratiometric signal to be detected.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-020-04680-2</identifier><identifier>PMID: 33502612</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Cell Line, Tumor ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; DNA - chemistry ; DNA - genetics ; DNA Probes - chemistry ; DNA Probes - genetics ; Enzymes ; Fluorescence ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; G-Quadruplexes ; Humans ; Inverted Repeat Sequences ; Investigations ; Limit of Detection ; Metal Nanoparticles - chemistry ; Microengineering ; MicroRNA ; MicroRNAs ; MicroRNAs - analysis ; MicroRNAs - genetics ; Molecular chains ; Nanochemistry ; Nanoclusters ; Nanotechnology ; Nucleic Acid Hybridization ; Original Paper ; Reproducibility of Results ; Ribonucleic acid ; RNA ; Silver - chemistry ; Spectrometry, Fluorescence - methods</subject><ispartof>Mikrochimica acta (1966), 2021-02, Vol.188 (2), p.55-55, Article 55</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, AT part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, AT part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-dac551cd138fb2f6abd3e6a1330ef76077a0ba99f7e462bc0e216d2145a5c5823</citedby><cites>FETCH-LOGICAL-c414t-dac551cd138fb2f6abd3e6a1330ef76077a0ba99f7e462bc0e216d2145a5c5823</cites><orcidid>0000-0003-3354-5069</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-020-04680-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-020-04680-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33502612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Xiaojia</creatorcontrib><creatorcontrib>Wang, Shuang</creatorcontrib><creatorcontrib>Zou, Rong</creatorcontrib><creatorcontrib>Chen, Chunyan</creatorcontrib><creatorcontrib>Cai, Changqun</creatorcontrib><title>An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A sensitive ratiometric fluorescence probe based on hybridization chain reaction (HCR) was constructed for sensitive detection of miRNA-21 by using G-triplex and silver nanocluster pairs (AgNC pairs) as an enzyme-free and label-free signal output group. miRNA-21 was used as the primer for the hybridization chain reaction of molecular beacon 1 (MB1) containing the locked G-triplex sequence and molecular beacon 2 (MB2) with intact AgNC pairs at the 5′ and 3′ end activation. The double-stranded product was obtained along with the opening of the G-triplex and the separation of the AgNC pairs. A detection limit of 67 pM and a linear detection range of 0.1–300 nM were obtained for miRNA-21 determination. The proposed strategy enabled the monitoring of miRNA-21 levels in at least three cell lines, indicating that it provided new ideas for detecting miRNA in real samples.
Graphical abstract
MB1 and MB2 contained the locked G-triplex sequence and silver nanocluster pairs (AgNC pairs), respectively. In the presence of target, the hybridization chain reaction (HCR) between MB1 and MB2 was initiated. At the same time, the locked G-triplex was released and combined to the dye thioflavin T (THT) to increase fluorescence, while the separation of the AgNC pairs caused the fluorescence to decrease. The double-stranded (ds) DNA product was generated to form a ratiometric signal to be detected.</description><subject>Analytical Chemistry</subject><subject>Cell Line, Tumor</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Probes - chemistry</subject><subject>DNA Probes - genetics</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>G-Quadruplexes</subject><subject>Humans</subject><subject>Inverted Repeat Sequences</subject><subject>Investigations</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microengineering</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - genetics</subject><subject>Molecular chains</subject><subject>Nanochemistry</subject><subject>Nanoclusters</subject><subject>Nanotechnology</subject><subject>Nucleic Acid Hybridization</subject><subject>Original Paper</subject><subject>Reproducibility of Results</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Silver - chemistry</subject><subject>Spectrometry, Fluorescence - methods</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rFTEUxYMo9ln9Ai4k4MZN6s2fSabLR9EqFAXRdchkbkrKTPJMZorPT2_aqQouJIvAub97OJdDyEsOZxzAvK0AGhQDAQyU7oGJR2THldSsAyMfkx2A0ExqI07Is1pvALjRQj0lJ1J2bcTFjtR9oph-HmdkoSDSQ8kD0sFVHGlO9JItJR4m_EFdrbEuTR2OtMbpFgtNLmU_rU0t9OBiqTTkQiumGpd4i3TEBf0Sm00OdI6-5C-f9kzw5-RJcFPFFw__Kfn2_t3Xiw_s6vPlx4v9FfOKq4WNzncd9yOXfRhE0G4YJWrHpQQMRoMxDgZ3fh4MKi0GDyi4HgVXnet81wt5St5svu2o7yvWxc6xepwmlzCv1QrVc620Mbqhr_9Bb_JaUkt3T_U9cFCNOtuoazehjSnkpTjf3ojtvJwwxKbvDe-g04ZDWxDbQru91oLBHkqcXTlaDvauQ7t1aFuH9r5Dexf71UOWdZhx_LPyu7QGyA2obZSusfwN-x_bX-YepmI</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Zhao, Xiaojia</creator><creator>Wang, Shuang</creator><creator>Zou, Rong</creator><creator>Chen, Chunyan</creator><creator>Cai, Changqun</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3354-5069</orcidid></search><sort><creationdate>20210201</creationdate><title>An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21</title><author>Zhao, Xiaojia ; Wang, Shuang ; Zou, Rong ; Chen, Chunyan ; Cai, Changqun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-dac551cd138fb2f6abd3e6a1330ef76077a0ba99f7e462bc0e216d2145a5c5823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Cell Line, Tumor</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Probes - chemistry</topic><topic>DNA Probes - genetics</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>G-Quadruplexes</topic><topic>Humans</topic><topic>Inverted Repeat Sequences</topic><topic>Investigations</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microengineering</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - analysis</topic><topic>MicroRNAs - genetics</topic><topic>Molecular chains</topic><topic>Nanochemistry</topic><topic>Nanoclusters</topic><topic>Nanotechnology</topic><topic>Nucleic Acid Hybridization</topic><topic>Original Paper</topic><topic>Reproducibility of Results</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Silver - chemistry</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Xiaojia</creatorcontrib><creatorcontrib>Wang, Shuang</creatorcontrib><creatorcontrib>Zou, Rong</creatorcontrib><creatorcontrib>Chen, Chunyan</creatorcontrib><creatorcontrib>Cai, Changqun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Xiaojia</au><au>Wang, Shuang</au><au>Zou, Rong</au><au>Chen, Chunyan</au><au>Cai, Changqun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>188</volume><issue>2</issue><spage>55</spage><epage>55</epage><pages>55-55</pages><artnum>55</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A sensitive ratiometric fluorescence probe based on hybridization chain reaction (HCR) was constructed for sensitive detection of miRNA-21 by using G-triplex and silver nanocluster pairs (AgNC pairs) as an enzyme-free and label-free signal output group. miRNA-21 was used as the primer for the hybridization chain reaction of molecular beacon 1 (MB1) containing the locked G-triplex sequence and molecular beacon 2 (MB2) with intact AgNC pairs at the 5′ and 3′ end activation. The double-stranded product was obtained along with the opening of the G-triplex and the separation of the AgNC pairs. A detection limit of 67 pM and a linear detection range of 0.1–300 nM were obtained for miRNA-21 determination. The proposed strategy enabled the monitoring of miRNA-21 levels in at least three cell lines, indicating that it provided new ideas for detecting miRNA in real samples.
Graphical abstract
MB1 and MB2 contained the locked G-triplex sequence and silver nanocluster pairs (AgNC pairs), respectively. In the presence of target, the hybridization chain reaction (HCR) between MB1 and MB2 was initiated. At the same time, the locked G-triplex was released and combined to the dye thioflavin T (THT) to increase fluorescence, while the separation of the AgNC pairs caused the fluorescence to decrease. The double-stranded (ds) DNA product was generated to form a ratiometric signal to be detected.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>33502612</pmid><doi>10.1007/s00604-020-04680-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3354-5069</orcidid></addata></record> |
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| ispartof | Mikrochimica acta (1966), 2021-02, Vol.188 (2), p.55-55, Article 55 |
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| subjects | Analytical Chemistry Cell Line, Tumor Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science DNA - chemistry DNA - genetics DNA Probes - chemistry DNA Probes - genetics Enzymes Fluorescence Fluorescent Dyes - chemistry Fluorescent indicators G-Quadruplexes Humans Inverted Repeat Sequences Investigations Limit of Detection Metal Nanoparticles - chemistry Microengineering MicroRNA MicroRNAs MicroRNAs - analysis MicroRNAs - genetics Molecular chains Nanochemistry Nanoclusters Nanotechnology Nucleic Acid Hybridization Original Paper Reproducibility of Results Ribonucleic acid RNA Silver - chemistry Spectrometry, Fluorescence - methods |
| title | An enzyme-free probe based on G-triplex assisted by silver nanocluster pairs for sensitive detection of microRNA-21 |
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