Approaches for the sensitive detection of rare base and prime editing events

•Novel, agarose gel-based detection methods for base and prime editing detection.•Workflow from transfection through to analysis for base and prime editing.•Base editing of plasmid DNA targets in mammalian cells. Precision chemistry entailing user-directed nucleotide substitutions and template-speci...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2021-10, Vol.194, p.75-82
Hauptverfasser:	Nguyen Tran, Minh Thuan, Rajendra, K.C., Patterson, Freya M., Liu, Guei-Sheung, Cook, Anthony L., Hewitt, Alex W.
Format:	Artikel
Sprache:	eng
Schlagworte:	ARMS Base editing Detection DNA Gene Editing Genome Genomics High throughput Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Prime editing RFLP
Online-Zugang:	Volltext
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