Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats
The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow–derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats. A total of 45 male Wistar al...
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| Veröffentlicht in: | The Journal of surgical research 2021-05, Vol.261, p.205-214 |
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| container_title | The Journal of surgical research |
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| creator | Demir, Sabri Erturk, Ahmet Gunal, Yasemin Dere Ozmen, Ismail Zengin, Mehmet Yildiz, Dincer Karaoz, Erdal Karahan, Siyami Senel, Emrah |
| description | The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow–derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats.
A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of |
| doi_str_mv | 10.1016/j.jss.2020.12.006 |
| format | Article |
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A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant.
BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II.
The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2020.12.006</identifier><identifier>PMID: 33450629</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acute Lung Injury - pathology ; Acute Lung Injury - therapy ; Animals ; Bone Marrow Transplantation ; Bone marrow-derived mesenchymal stem cells ; Cells, Cultured ; Children ; Contusions - therapy ; Lung - pathology ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stem Cells - cytology ; Microscopy, Fluorescence ; Pulmonary contusion ; Rats ; Rats, Wistar ; Thoracic injury ; Trauma ; Wound Healing</subject><ispartof>The Journal of surgical research, 2021-05, Vol.261, p.205-214</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c268t-484e00217cb4845e67038c8ba49b9b058ab67fe3158c8e91a92869ed13998e4e3</citedby><cites>FETCH-LOGICAL-c268t-484e00217cb4845e67038c8ba49b9b058ab67fe3158c8e91a92869ed13998e4e3</cites><orcidid>0000-0003-4720-912X ; 0000-0003-0286-362X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jss.2020.12.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33450629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demir, Sabri</creatorcontrib><creatorcontrib>Erturk, Ahmet</creatorcontrib><creatorcontrib>Gunal, Yasemin Dere</creatorcontrib><creatorcontrib>Ozmen, Ismail</creatorcontrib><creatorcontrib>Zengin, Mehmet</creatorcontrib><creatorcontrib>Yildiz, Dincer</creatorcontrib><creatorcontrib>Karaoz, Erdal</creatorcontrib><creatorcontrib>Karahan, Siyami</creatorcontrib><creatorcontrib>Senel, Emrah</creatorcontrib><title>Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow–derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats.
A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant.
BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II.
The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods.</description><subject>Acute Lung Injury - pathology</subject><subject>Acute Lung Injury - therapy</subject><subject>Animals</subject><subject>Bone Marrow Transplantation</subject><subject>Bone marrow-derived mesenchymal stem cells</subject><subject>Cells, Cultured</subject><subject>Children</subject><subject>Contusions - therapy</subject><subject>Lung - pathology</subject><subject>Male</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Microscopy, Fluorescence</subject><subject>Pulmonary contusion</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Thoracic injury</subject><subject>Trauma</subject><subject>Wound Healing</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3DAUhS3UCgbKA7BBXnaTwXYSx1ZXEChUAoH4WVtOcgc8JDHYDhU73qFvyJNwR0O77Mq-V-d8OvcQssfZnDMuD5bzZYxzwQTOYs6Y3CAzznSZKVnlX8iMMSGyQrFii2zHuGQ46yrfJFt5XpRMCj0jj7UfU3DNlJwfqV_QIz8CvbAh-N_vb3-OIbgX6OgFRBjbh9fB9vQmwUBr6PtIk6dnYHs33q-sV1M_-NGGV7qCThGJWR3AJgRc2xS_ka8L20fY_Xx3yN3Pk9v6LDu_PP1VH55nrZAqYeACMCmv2ga_JciK5apVjS10oxtWKtvIagE5L3ELmlstlNTQ8VxrBQXkO-T7mvsU_PMEMZnBxRYD2xH8FI0oKlXqSkiGUr6WtsHHGGBhnoIb8ATDmVl1bJYGOzarjg0XBjtGz_4nfmoG6P45_paKgh9rAeCRLw6Cia3D-qBzAdpkOu_-g_8A6hONlw</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Demir, Sabri</creator><creator>Erturk, Ahmet</creator><creator>Gunal, Yasemin Dere</creator><creator>Ozmen, Ismail</creator><creator>Zengin, Mehmet</creator><creator>Yildiz, Dincer</creator><creator>Karaoz, Erdal</creator><creator>Karahan, Siyami</creator><creator>Senel, Emrah</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4720-912X</orcidid><orcidid>https://orcid.org/0000-0003-0286-362X</orcidid></search><sort><creationdate>202105</creationdate><title>Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats</title><author>Demir, Sabri ; Erturk, Ahmet ; Gunal, Yasemin Dere ; Ozmen, Ismail ; Zengin, Mehmet ; Yildiz, Dincer ; Karaoz, Erdal ; Karahan, Siyami ; Senel, Emrah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-484e00217cb4845e67038c8ba49b9b058ab67fe3158c8e91a92869ed13998e4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acute Lung Injury - pathology</topic><topic>Acute Lung Injury - therapy</topic><topic>Animals</topic><topic>Bone Marrow Transplantation</topic><topic>Bone marrow-derived mesenchymal stem cells</topic><topic>Cells, Cultured</topic><topic>Children</topic><topic>Contusions - therapy</topic><topic>Lung - pathology</topic><topic>Male</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Microscopy, Fluorescence</topic><topic>Pulmonary contusion</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Thoracic injury</topic><topic>Trauma</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demir, Sabri</creatorcontrib><creatorcontrib>Erturk, Ahmet</creatorcontrib><creatorcontrib>Gunal, Yasemin Dere</creatorcontrib><creatorcontrib>Ozmen, Ismail</creatorcontrib><creatorcontrib>Zengin, Mehmet</creatorcontrib><creatorcontrib>Yildiz, Dincer</creatorcontrib><creatorcontrib>Karaoz, Erdal</creatorcontrib><creatorcontrib>Karahan, Siyami</creatorcontrib><creatorcontrib>Senel, Emrah</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demir, Sabri</au><au>Erturk, Ahmet</au><au>Gunal, Yasemin Dere</au><au>Ozmen, Ismail</au><au>Zengin, Mehmet</au><au>Yildiz, Dincer</au><au>Karaoz, Erdal</au><au>Karahan, Siyami</au><au>Senel, Emrah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2021-05</date><risdate>2021</risdate><volume>261</volume><spage>205</spage><epage>214</epage><pages>205-214</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><abstract>The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow–derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats.
A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant.
BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II.
The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33450629</pmid><doi>10.1016/j.jss.2020.12.006</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4720-912X</orcidid><orcidid>https://orcid.org/0000-0003-0286-362X</orcidid></addata></record> |
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| subjects | Acute Lung Injury - pathology Acute Lung Injury - therapy Animals Bone Marrow Transplantation Bone marrow-derived mesenchymal stem cells Cells, Cultured Children Contusions - therapy Lung - pathology Male Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells - cytology Microscopy, Fluorescence Pulmonary contusion Rats Rats, Wistar Thoracic injury Trauma Wound Healing |
| title | Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats |
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