Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation

In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the...

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Veröffentlicht in:Zygote (Cambridge) 2021-06, Vol.29 (3), p.1-222
Hauptverfasser: Rola, Luciana Diniz, Zanetti, Eveline Dos Santos, Del Collado, Maite, Peroni, Ellen de Fátima Carvalho, Duarte, José Maurício Barbanti
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container_issue 3
container_start_page 1
container_title Zygote (Cambridge)
container_volume 29
creator Rola, Luciana Diniz
Zanetti, Eveline Dos Santos
Del Collado, Maite
Peroni, Ellen de Fátima Carvalho
Duarte, José Maurício Barbanti
description In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature.
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ispartof Zygote (Cambridge), 2021-06, Vol.29 (3), p.1-222
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source Cambridge University Press Journals Complete
subjects 17β-Estradiol
Benzoates
Collection
Embryos
Endangered & extinct species
Evaluation
Females
Follicle-stimulating hormone
Follicles
Gametocytes
Genetic diversity
Hormones
In vitro methods and tests
Laparoscopy
Laparotomy
Maturation
Mazama gouazoubira
Oocytes
Ovaries
Parthenogenesis
Protocol
Sex hormones
Stimulation
Surgery
Wildlife conservation
title Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation
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