The relevance of miRNAs as promising biomarkers in lip cancer
Objectives This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformatio...
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| Veröffentlicht in: | Clinical oral investigations 2021-07, Vol.25 (7), p.4591-4598 |
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| container_title | Clinical oral investigations |
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| creator | Assao, Agnes Domingues, Maria Aparecida Custódio Minicucci, Eliana Maria Marchi, Fabio Albuquerque Coutinho-Camillo, Cláudia Malheiros Oliveira, Denise Tostes |
| description | Objectives
This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation.
Materials and methods
The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square.
Results
A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers.
Conclusions
The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
Clinical relevance
This miRNA signature can help to identify actinic cheilitis with potential to progress to lip cancer. |
| doi_str_mv | 10.1007/s00784-020-03773-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2477512388</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2477512388</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-5323cec2d9565d4589fd25d96da597e1aaf872b78377581dc9476106bab356163</originalsourceid><addsrcrecordid>eNp9kElLAzEAhYMotlb_gAcJePEymn05eCjFDYqC1HPIZDJ16nSmJh3Bf2_aqQoePGQj33t5eQCcYnSJEZJXMU2KZYigDFEpaab3wBAzKrJ0wvvbPcmEVngAjmJcIISZkPQQDChlVKcxBNezVw-Dr_2HbZyHbQmX1fPjOEIb4Sq0yypWzRzmVbu04c2HCKsG1tUKug0ejsFBaevoT3brCLzc3swm99n06e5hMp5mjkq-zjgl1HlHCs0FLxhXuiwIL7QoLNfSY2tLJUkuVcrNFS6cZlJgJHKbUy6woCNw0fumSO-dj2uTgjlf17bxbRcNYUmICVUqoed_0EXbhSalM4RzJoRkgiWK9JQLbYzBl2YVqvTFT4OR2ZRr-nJNKtdsyzU6ic521l2-9MWP5LvNBNAeiOmqmfvw-_Y_tl-eA4Hy</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2554667464</pqid></control><display><type>article</type><title>The relevance of miRNAs as promising biomarkers in lip cancer</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Assao, Agnes ; Domingues, Maria Aparecida Custódio ; Minicucci, Eliana Maria ; Marchi, Fabio Albuquerque ; Coutinho-Camillo, Cláudia Malheiros ; Oliveira, Denise Tostes</creator><creatorcontrib>Assao, Agnes ; Domingues, Maria Aparecida Custódio ; Minicucci, Eliana Maria ; Marchi, Fabio Albuquerque ; Coutinho-Camillo, Cláudia Malheiros ; Oliveira, Denise Tostes</creatorcontrib><description>Objectives
This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation.
Materials and methods
The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square.
Results
A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers.
Conclusions
The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
Clinical relevance
This miRNA signature can help to identify actinic cheilitis with potential to progress to lip cancer.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-020-03773-9</identifier><identifier>PMID: 33439343</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomarkers ; Cancer ; Cheilitis - genetics ; Dentistry ; Dysplasia ; Humans ; Lip ; Lip Neoplasms - genetics ; Medicine ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Mucosa ; Oral cancer ; Oral carcinoma ; Original Article ; Paraffin ; Polymerase chain reaction ; Squamous cell carcinoma</subject><ispartof>Clinical oral investigations, 2021-07, Vol.25 (7), p.4591-4598</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-5323cec2d9565d4589fd25d96da597e1aaf872b78377581dc9476106bab356163</citedby><cites>FETCH-LOGICAL-c375t-5323cec2d9565d4589fd25d96da597e1aaf872b78377581dc9476106bab356163</cites><orcidid>0000-0002-4628-7129</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-020-03773-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-020-03773-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33439343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Assao, Agnes</creatorcontrib><creatorcontrib>Domingues, Maria Aparecida Custódio</creatorcontrib><creatorcontrib>Minicucci, Eliana Maria</creatorcontrib><creatorcontrib>Marchi, Fabio Albuquerque</creatorcontrib><creatorcontrib>Coutinho-Camillo, Cláudia Malheiros</creatorcontrib><creatorcontrib>Oliveira, Denise Tostes</creatorcontrib><title>The relevance of miRNAs as promising biomarkers in lip cancer</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation.
Materials and methods
The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square.
Results
A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers.
Conclusions
The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
Clinical relevance
This miRNA signature can help to identify actinic cheilitis with potential to progress to lip cancer.</description><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cheilitis - genetics</subject><subject>Dentistry</subject><subject>Dysplasia</subject><subject>Humans</subject><subject>Lip</subject><subject>Lip Neoplasms - genetics</subject><subject>Medicine</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Mucosa</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>Original Article</subject><subject>Paraffin</subject><subject>Polymerase chain reaction</subject><subject>Squamous cell carcinoma</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kElLAzEAhYMotlb_gAcJePEymn05eCjFDYqC1HPIZDJ16nSmJh3Bf2_aqQoePGQj33t5eQCcYnSJEZJXMU2KZYigDFEpaab3wBAzKrJ0wvvbPcmEVngAjmJcIISZkPQQDChlVKcxBNezVw-Dr_2HbZyHbQmX1fPjOEIb4Sq0yypWzRzmVbu04c2HCKsG1tUKug0ejsFBaevoT3brCLzc3swm99n06e5hMp5mjkq-zjgl1HlHCs0FLxhXuiwIL7QoLNfSY2tLJUkuVcrNFS6cZlJgJHKbUy6woCNw0fumSO-dj2uTgjlf17bxbRcNYUmICVUqoed_0EXbhSalM4RzJoRkgiWK9JQLbYzBl2YVqvTFT4OR2ZRr-nJNKtdsyzU6ic521l2-9MWP5LvNBNAeiOmqmfvw-_Y_tl-eA4Hy</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Assao, Agnes</creator><creator>Domingues, Maria Aparecida Custódio</creator><creator>Minicucci, Eliana Maria</creator><creator>Marchi, Fabio Albuquerque</creator><creator>Coutinho-Camillo, Cláudia Malheiros</creator><creator>Oliveira, Denise Tostes</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4628-7129</orcidid></search><sort><creationdate>20210701</creationdate><title>The relevance of miRNAs as promising biomarkers in lip cancer</title><author>Assao, Agnes ; Domingues, Maria Aparecida Custódio ; Minicucci, Eliana Maria ; Marchi, Fabio Albuquerque ; Coutinho-Camillo, Cláudia Malheiros ; Oliveira, Denise Tostes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-5323cec2d9565d4589fd25d96da597e1aaf872b78377581dc9476106bab356163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomarkers</topic><topic>Cancer</topic><topic>Cheilitis - genetics</topic><topic>Dentistry</topic><topic>Dysplasia</topic><topic>Humans</topic><topic>Lip</topic><topic>Lip Neoplasms - genetics</topic><topic>Medicine</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Mucosa</topic><topic>Oral cancer</topic><topic>Oral carcinoma</topic><topic>Original Article</topic><topic>Paraffin</topic><topic>Polymerase chain reaction</topic><topic>Squamous cell carcinoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Assao, Agnes</creatorcontrib><creatorcontrib>Domingues, Maria Aparecida Custódio</creatorcontrib><creatorcontrib>Minicucci, Eliana Maria</creatorcontrib><creatorcontrib>Marchi, Fabio Albuquerque</creatorcontrib><creatorcontrib>Coutinho-Camillo, Cláudia Malheiros</creatorcontrib><creatorcontrib>Oliveira, Denise Tostes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Assao, Agnes</au><au>Domingues, Maria Aparecida Custódio</au><au>Minicucci, Eliana Maria</au><au>Marchi, Fabio Albuquerque</au><au>Coutinho-Camillo, Cláudia Malheiros</au><au>Oliveira, Denise Tostes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The relevance of miRNAs as promising biomarkers in lip cancer</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>25</volume><issue>7</issue><spage>4591</spage><epage>4598</epage><pages>4591-4598</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
This study aimed to analyze the expression of miR-181b, miR-21, miR-31, and miR-345 in actinic cheilitis with and without epithelial dysplasia and lower lip squamous cell carcinomas, and to verify if the deregulated expression of these miRNAs would be indicative of malignant transformation.
Materials and methods
The sample was selected from formalin-fixed paraffin-embedded tissues of 19 actinic cheilitis without epithelial dysplasia, 32 actinic cheilitis with epithelial dysplasia, 42 lower lip squamous cell carcinomas, and 10 nonaltered oral mucosa of the lip. The microRNA (miR, miRNA) expression was quantified by real-time RT-PCR and the expression of the selected miRNAs among the groups of actinic cheilitis and lower lip cancer was compared by chi-square.
Results
A higher expression of miR-181b, miR-31, and miR-345 was found in actinic cheilitis without epithelial dysplasia in comparison to that in actinic cheilitis with epithelial dysplasia and with lower lip cancer. There were no differences in miR-21 expression between actinic cheilitis and lower lip cancer. Hierarchical clustering analysis showed a tendency for a downregulation of miR-181b, miR-21, miR-31, and miR-345 in most patients with lower lip cancers.
Conclusions
The upregulation of miR-181b, miR-31, and miR-345 expression in actinic cheilitis without epithelial dysplasia and the decrease in the expression of these miRNAs in actinic cheilitis with epithelial dysplasia and in lower lip cancer are potential biomarkers of malignant progression.
Clinical relevance
This miRNA signature can help to identify actinic cheilitis with potential to progress to lip cancer.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33439343</pmid><doi>10.1007/s00784-020-03773-9</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4628-7129</orcidid></addata></record> |
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| language | eng |
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| source | MEDLINE; SpringerLink (Online service) |
| subjects | Biomarkers Cancer Cheilitis - genetics Dentistry Dysplasia Humans Lip Lip Neoplasms - genetics Medicine MicroRNAs MicroRNAs - genetics miRNA Mucosa Oral cancer Oral carcinoma Original Article Paraffin Polymerase chain reaction Squamous cell carcinoma |
| title | The relevance of miRNAs as promising biomarkers in lip cancer |
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