Human sperm tsRNA as potential biomarker and therapy target for male fertility
Infertility caused by male factors is routinely diagnosed by assessing traditional semen parameters. Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNAGln-TTG deriv...
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| Veröffentlicht in: | Reproduction (Cambridge, England) England), 2021-02, Vol.161 (2), p.111-122 |
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| creator | Chen, Xiaoxu Sun, Qian Zheng, Yi Liu, Zidong Meng, Xiangqian Zeng, Wenxian Lu, Hongzhao |
| description | Infertility caused by male factors is routinely diagnosed by assessing traditional semen parameters. Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNAGln-TTG derived small RNAs played notable roles in the first cleavage of a porcine embryo. However, the function of human sperm tRNAGln-TTG derived small RNAs as a diagnostic biomarker and its role in early embryo development remains unclear. In this study, we found that human sperm tRNAGln-TTG derived small RNAs were highly associated with sperm quality. By microinjecting the antisense sequence into human tripronuclear (3PN) zygotes followed by single-cell RNA-sequencing, we found that human sperm tRNAGln-TTG derived small RNAs participated in the development of a human embryo. Furthermore, Gln-TTGs might influence embryonic genome activation by modulating noncoding RNA processing. These findings demonstrated that human sperm tRNAGln-TTG derived small RNAs could be potential diagnostic biomarkers and could be used as a clinical target for male infertility. |
| doi_str_mv | 10.1530/REP-20-0415 |
| format | Article |
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Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNAGln-TTG derived small RNAs played notable roles in the first cleavage of a porcine embryo. However, the function of human sperm tRNAGln-TTG derived small RNAs as a diagnostic biomarker and its role in early embryo development remains unclear. In this study, we found that human sperm tRNAGln-TTG derived small RNAs were highly associated with sperm quality. By microinjecting the antisense sequence into human tripronuclear (3PN) zygotes followed by single-cell RNA-sequencing, we found that human sperm tRNAGln-TTG derived small RNAs participated in the development of a human embryo. Furthermore, Gln-TTGs might influence embryonic genome activation by modulating noncoding RNA processing. 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Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNAGln-TTG derived small RNAs played notable roles in the first cleavage of a porcine embryo. However, the function of human sperm tRNAGln-TTG derived small RNAs as a diagnostic biomarker and its role in early embryo development remains unclear. In this study, we found that human sperm tRNAGln-TTG derived small RNAs were highly associated with sperm quality. By microinjecting the antisense sequence into human tripronuclear (3PN) zygotes followed by single-cell RNA-sequencing, we found that human sperm tRNAGln-TTG derived small RNAs participated in the development of a human embryo. Furthermore, Gln-TTGs might influence embryonic genome activation by modulating noncoding RNA processing. These findings demonstrated that human sperm tRNAGln-TTG derived small RNAs could be potential diagnostic biomarkers and could be used as a clinical target for male infertility.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Fertility</subject><subject>Humans</subject><subject>Male</subject><subject>RNA - genetics</subject><subject>Semen</subject><subject>Spermatozoa</subject><subject>Swine</subject><issn>1470-1626</issn><issn>1741-7899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LwzAUgIMobk5P3iVHQar51aQ9jjGdIFOGnsNbm2i0XWuSHvbfm7Hp0dN7PD4-eB9Cl5Tc0pyTu9X8JWMkI4LmR2hMlaCZKsryOO1CkYxKJkfoLIRPQmheKHmKRpwLnvByjJaLoYUNDr3xLY5htZxiCLjvotlEBw1eu64F_2U8hk2N44fx0G9xBP9uIradxy00Blvjo2tc3J6jEwtNMBeHOUFv9_PX2SJ7en54nE2fsjWXRcxKWRIlwDJRgiwIV6wiJaGiVJwQCWDydGA5l1AxUdii5lIxIYXlBbe2pnyCrvfe3nffgwlRty5UpmlgY7ohaCaUSjaZ5wm92aOV70Lwxureu_TTVlOidwF1CqgZ0buAib46iId1a-o_9rdYAugeSGVC5XaZrKvgX-kPDad5qQ</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Chen, Xiaoxu</creator><creator>Sun, Qian</creator><creator>Zheng, Yi</creator><creator>Liu, Zidong</creator><creator>Meng, Xiangqian</creator><creator>Zeng, Wenxian</creator><creator>Lu, Hongzhao</creator><general>Bioscientifica Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202102</creationdate><title>Human sperm tsRNA as potential biomarker and therapy target for male fertility</title><author>Chen, Xiaoxu ; Sun, Qian ; Zheng, Yi ; Liu, Zidong ; Meng, Xiangqian ; Zeng, Wenxian ; Lu, Hongzhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b368t-969074af249a680372c09014973006aae52c02536ac248f8d3672464f383ffd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Fertility</topic><topic>Humans</topic><topic>Male</topic><topic>RNA - genetics</topic><topic>Semen</topic><topic>Spermatozoa</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xiaoxu</creatorcontrib><creatorcontrib>Sun, Qian</creatorcontrib><creatorcontrib>Zheng, Yi</creatorcontrib><creatorcontrib>Liu, Zidong</creatorcontrib><creatorcontrib>Meng, Xiangqian</creatorcontrib><creatorcontrib>Zeng, Wenxian</creatorcontrib><creatorcontrib>Lu, Hongzhao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xiaoxu</au><au>Sun, Qian</au><au>Zheng, Yi</au><au>Liu, Zidong</au><au>Meng, Xiangqian</au><au>Zeng, Wenxian</au><au>Lu, Hongzhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human sperm tsRNA as potential biomarker and therapy target for male fertility</atitle><jtitle>Reproduction (Cambridge, England)</jtitle><addtitle>Reproduction</addtitle><date>2021-02</date><risdate>2021</risdate><volume>161</volume><issue>2</issue><spage>111</spage><epage>122</epage><pages>111-122</pages><issn>1470-1626</issn><eissn>1741-7899</eissn><abstract>Infertility caused by male factors is routinely diagnosed by assessing traditional semen parameters. Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNAGln-TTG derived small RNAs played notable roles in the first cleavage of a porcine embryo. However, the function of human sperm tRNAGln-TTG derived small RNAs as a diagnostic biomarker and its role in early embryo development remains unclear. In this study, we found that human sperm tRNAGln-TTG derived small RNAs were highly associated with sperm quality. By microinjecting the antisense sequence into human tripronuclear (3PN) zygotes followed by single-cell RNA-sequencing, we found that human sperm tRNAGln-TTG derived small RNAs participated in the development of a human embryo. Furthermore, Gln-TTGs might influence embryonic genome activation by modulating noncoding RNA processing. 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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biomarkers Fertility Humans Male RNA - genetics Semen Spermatozoa Swine |
| title | Human sperm tsRNA as potential biomarker and therapy target for male fertility |
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