JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis
• Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear. • Here, we show that a histone 3 lys...
Gespeichert in:
| Veröffentlicht in: | The New phytologist 2021-04, Vol.230 (2), p.567-584 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 584 |
|---|---|
| container_issue | 2 |
| container_start_page | 567 |
| container_title | The New phytologist |
| container_volume | 230 |
| creator | Wang, Tian-Jing Huang, Shuangzhan Zhang, Ai Guo, Peng Liu, Yutong Xu, Chunming Cong, Weixuan Liu, Bao Xu, Zheng-Yi |
| description | • Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear.
• Here, we show that a histone 3 lysine 4 (H3K4) demethylase, JMJ17, regulates the expression of ABA-responsive genes during seed germination and seedling growth. Using comparative interactomics, WRKY40, a central transcriptional repressor in ABA signaling, was shown to interact with JMJ17. WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.
• Additionally, WRKY40 represses the transcriptional activation activity of HY5, which can activate ABI5 expression by directly binding to its promoter. An increase in ABA concentrations decreases the affinity of WRKY40 for the ABI5 promoter. Thus, WRKY40 and JMJ17 are released from the ABI5 chromatin, activating HY5. The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.
• Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis. |
| doi_str_mv | 10.1111/nph.17177 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2476849015</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>27013898</jstor_id><sourcerecordid>27013898</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4107-4aebd1be931e05c1126e99869f4bd410c713e3c959d921556011337dca65d3663</originalsourceid><addsrcrecordid>eNp10Mtu1DAUBmALgei0sOABQJbYwCKtj-9ephVlWspFCARdRUl8ps0oEwd70suu78Ab8iQYpu0CCW8sW9_5dfQT8gzYLuSzN4znu2DAmAdkBlK7woIwD8mMMW4LLfX3LbKd0pIx5pTmj8mWEJILAWpG_PH7YzC_bn5--_zuVDJaD57OT1X-KPePFF0FP_WYaMSzqa_XSNfnSPFqjJhSFwYaFrTcL4v8HMOQugukZzhk3w20jHXT-TCmLj0hjxZ1n_Dp7b1Dvh6--XIwL04-vj06KE-KVgIzhayx8dCgE4BMtQBco3NWu4VsfBatAYGidcp5x0EpzQCEML6ttfJCa7FDXm1yxxh-TJjW1apLLfZ9PWCYUsWl0VY6BirTl__QZZjikLeruGJgjbXcZvV6o9oYUoq4qMbYrep4XQGr_lRf5eqrv9Vn--I2cWpW6O_lXdcZ7G3AZdfj9f-Tqg-f5neRzzcTy7QO8X6CGwbCOit-A93YlV8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2501878828</pqid></control><display><type>article</type><title>JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Wang, Tian-Jing ; Huang, Shuangzhan ; Zhang, Ai ; Guo, Peng ; Liu, Yutong ; Xu, Chunming ; Cong, Weixuan ; Liu, Bao ; Xu, Zheng-Yi</creator><creatorcontrib>Wang, Tian-Jing ; Huang, Shuangzhan ; Zhang, Ai ; Guo, Peng ; Liu, Yutong ; Xu, Chunming ; Cong, Weixuan ; Liu, Bao ; Xu, Zheng-Yi</creatorcontrib><description>• Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear.
• Here, we show that a histone 3 lysine 4 (H3K4) demethylase, JMJ17, regulates the expression of ABA-responsive genes during seed germination and seedling growth. Using comparative interactomics, WRKY40, a central transcriptional repressor in ABA signaling, was shown to interact with JMJ17. WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.
• Additionally, WRKY40 represses the transcriptional activation activity of HY5, which can activate ABI5 expression by directly binding to its promoter. An increase in ABA concentrations decreases the affinity of WRKY40 for the ABI5 promoter. Thus, WRKY40 and JMJ17 are released from the ABI5 chromatin, activating HY5. The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.
• Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.17177</identifier><identifier>PMID: 33423315</identifier><language>eng</language><publisher>England: Wiley</publisher><subject>Abscisic acid ; abscisic acid (ABA) ; Abscisic Acid - pharmacology ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Basic-Leucine Zipper Transcription Factors - genetics ; Basic-Leucine Zipper Transcription Factors - metabolism ; Chromatin ; Environmental stress ; Epigenetics ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Germination ; histone methylation ; Histones ; Lysine ; Modules ; Promoters ; Seed germination ; Seedlings ; Seeds - metabolism ; Signal Transduction ; Transcription activation ; transcription factor ; Transcription Factors - genetics ; transcriptional regulation</subject><ispartof>The New phytologist, 2021-04, Vol.230 (2), p.567-584</ispartof><rights>2021 The Authors © 2020 New Phytologist Foundation</rights><rights>2021 The Authors. © 2020 New Phytologist Foundation</rights><rights>2021 The Authors. New Phytologist © 2020 New Phytologist Foundation.</rights><rights>Copyright © 2021 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4107-4aebd1be931e05c1126e99869f4bd410c713e3c959d921556011337dca65d3663</citedby><cites>FETCH-LOGICAL-c4107-4aebd1be931e05c1126e99869f4bd410c713e3c959d921556011337dca65d3663</cites><orcidid>0000-0001-5481-1675 ; 0000-0001-9820-2329 ; 0000-0003-3303-5851 ; 0000-0002-6145-0318 ; 0000-0002-0986-0993 ; 0000-0002-4802-3514 ; 0000-0001-8682-4163 ; 0000-0003-4822-1657 ; 0000-0002-8308-9973</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.17177$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.17177$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33423315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Tian-Jing</creatorcontrib><creatorcontrib>Huang, Shuangzhan</creatorcontrib><creatorcontrib>Zhang, Ai</creatorcontrib><creatorcontrib>Guo, Peng</creatorcontrib><creatorcontrib>Liu, Yutong</creatorcontrib><creatorcontrib>Xu, Chunming</creatorcontrib><creatorcontrib>Cong, Weixuan</creatorcontrib><creatorcontrib>Liu, Bao</creatorcontrib><creatorcontrib>Xu, Zheng-Yi</creatorcontrib><title>JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>• Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear.
• Here, we show that a histone 3 lysine 4 (H3K4) demethylase, JMJ17, regulates the expression of ABA-responsive genes during seed germination and seedling growth. Using comparative interactomics, WRKY40, a central transcriptional repressor in ABA signaling, was shown to interact with JMJ17. WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.
• Additionally, WRKY40 represses the transcriptional activation activity of HY5, which can activate ABI5 expression by directly binding to its promoter. An increase in ABA concentrations decreases the affinity of WRKY40 for the ABI5 promoter. Thus, WRKY40 and JMJ17 are released from the ABI5 chromatin, activating HY5. The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.
• Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.</description><subject>Abscisic acid</subject><subject>abscisic acid (ABA)</subject><subject>Abscisic Acid - pharmacology</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Basic-Leucine Zipper Transcription Factors - genetics</subject><subject>Basic-Leucine Zipper Transcription Factors - metabolism</subject><subject>Chromatin</subject><subject>Environmental stress</subject><subject>Epigenetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Germination</subject><subject>histone methylation</subject><subject>Histones</subject><subject>Lysine</subject><subject>Modules</subject><subject>Promoters</subject><subject>Seed germination</subject><subject>Seedlings</subject><subject>Seeds - metabolism</subject><subject>Signal Transduction</subject><subject>Transcription activation</subject><subject>transcription factor</subject><subject>Transcription Factors - genetics</subject><subject>transcriptional regulation</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10Mtu1DAUBmALgei0sOABQJbYwCKtj-9ephVlWspFCARdRUl8ps0oEwd70suu78Ab8iQYpu0CCW8sW9_5dfQT8gzYLuSzN4znu2DAmAdkBlK7woIwD8mMMW4LLfX3LbKd0pIx5pTmj8mWEJILAWpG_PH7YzC_bn5--_zuVDJaD57OT1X-KPePFF0FP_WYaMSzqa_XSNfnSPFqjJhSFwYaFrTcL4v8HMOQugukZzhk3w20jHXT-TCmLj0hjxZ1n_Dp7b1Dvh6--XIwL04-vj06KE-KVgIzhayx8dCgE4BMtQBco3NWu4VsfBatAYGidcp5x0EpzQCEML6ttfJCa7FDXm1yxxh-TJjW1apLLfZ9PWCYUsWl0VY6BirTl__QZZjikLeruGJgjbXcZvV6o9oYUoq4qMbYrep4XQGr_lRf5eqrv9Vn--I2cWpW6O_lXdcZ7G3AZdfj9f-Tqg-f5neRzzcTy7QO8X6CGwbCOit-A93YlV8</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Wang, Tian-Jing</creator><creator>Huang, Shuangzhan</creator><creator>Zhang, Ai</creator><creator>Guo, Peng</creator><creator>Liu, Yutong</creator><creator>Xu, Chunming</creator><creator>Cong, Weixuan</creator><creator>Liu, Bao</creator><creator>Xu, Zheng-Yi</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5481-1675</orcidid><orcidid>https://orcid.org/0000-0001-9820-2329</orcidid><orcidid>https://orcid.org/0000-0003-3303-5851</orcidid><orcidid>https://orcid.org/0000-0002-6145-0318</orcidid><orcidid>https://orcid.org/0000-0002-0986-0993</orcidid><orcidid>https://orcid.org/0000-0002-4802-3514</orcidid><orcidid>https://orcid.org/0000-0001-8682-4163</orcidid><orcidid>https://orcid.org/0000-0003-4822-1657</orcidid><orcidid>https://orcid.org/0000-0002-8308-9973</orcidid></search><sort><creationdate>20210401</creationdate><title>JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis</title><author>Wang, Tian-Jing ; Huang, Shuangzhan ; Zhang, Ai ; Guo, Peng ; Liu, Yutong ; Xu, Chunming ; Cong, Weixuan ; Liu, Bao ; Xu, Zheng-Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4107-4aebd1be931e05c1126e99869f4bd410c713e3c959d921556011337dca65d3663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abscisic acid</topic><topic>abscisic acid (ABA)</topic><topic>Abscisic Acid - pharmacology</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Basic-Leucine Zipper Transcription Factors - genetics</topic><topic>Basic-Leucine Zipper Transcription Factors - metabolism</topic><topic>Chromatin</topic><topic>Environmental stress</topic><topic>Epigenetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Germination</topic><topic>histone methylation</topic><topic>Histones</topic><topic>Lysine</topic><topic>Modules</topic><topic>Promoters</topic><topic>Seed germination</topic><topic>Seedlings</topic><topic>Seeds - metabolism</topic><topic>Signal Transduction</topic><topic>Transcription activation</topic><topic>transcription factor</topic><topic>Transcription Factors - genetics</topic><topic>transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Tian-Jing</creatorcontrib><creatorcontrib>Huang, Shuangzhan</creatorcontrib><creatorcontrib>Zhang, Ai</creatorcontrib><creatorcontrib>Guo, Peng</creatorcontrib><creatorcontrib>Liu, Yutong</creatorcontrib><creatorcontrib>Xu, Chunming</creatorcontrib><creatorcontrib>Cong, Weixuan</creatorcontrib><creatorcontrib>Liu, Bao</creatorcontrib><creatorcontrib>Xu, Zheng-Yi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Tian-Jing</au><au>Huang, Shuangzhan</au><au>Zhang, Ai</au><au>Guo, Peng</au><au>Liu, Yutong</au><au>Xu, Chunming</au><au>Cong, Weixuan</au><au>Liu, Bao</au><au>Xu, Zheng-Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>230</volume><issue>2</issue><spage>567</spage><epage>584</epage><pages>567-584</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>• Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear.
• Here, we show that a histone 3 lysine 4 (H3K4) demethylase, JMJ17, regulates the expression of ABA-responsive genes during seed germination and seedling growth. Using comparative interactomics, WRKY40, a central transcriptional repressor in ABA signaling, was shown to interact with JMJ17. WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.
• Additionally, WRKY40 represses the transcriptional activation activity of HY5, which can activate ABI5 expression by directly binding to its promoter. An increase in ABA concentrations decreases the affinity of WRKY40 for the ABI5 promoter. Thus, WRKY40 and JMJ17 are released from the ABI5 chromatin, activating HY5. The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.
• Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.</abstract><cop>England</cop><pub>Wiley</pub><pmid>33423315</pmid><doi>10.1111/nph.17177</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0001-5481-1675</orcidid><orcidid>https://orcid.org/0000-0001-9820-2329</orcidid><orcidid>https://orcid.org/0000-0003-3303-5851</orcidid><orcidid>https://orcid.org/0000-0002-6145-0318</orcidid><orcidid>https://orcid.org/0000-0002-0986-0993</orcidid><orcidid>https://orcid.org/0000-0002-4802-3514</orcidid><orcidid>https://orcid.org/0000-0001-8682-4163</orcidid><orcidid>https://orcid.org/0000-0003-4822-1657</orcidid><orcidid>https://orcid.org/0000-0002-8308-9973</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-646X |
| ispartof | The New phytologist, 2021-04, Vol.230 (2), p.567-584 |
| issn | 0028-646X 1469-8137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2476849015 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals |
| subjects | Abscisic acid abscisic acid (ABA) Abscisic Acid - pharmacology Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Basic-Leucine Zipper Transcription Factors - genetics Basic-Leucine Zipper Transcription Factors - metabolism Chromatin Environmental stress Epigenetics Gene expression Gene Expression Regulation, Plant Genes Germination histone methylation Histones Lysine Modules Promoters Seed germination Seedlings Seeds - metabolism Signal Transduction Transcription activation transcription factor Transcription Factors - genetics transcriptional regulation |
| title | JMJ17–WRKY40 and HY5–ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T03%3A40%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=JMJ17%E2%80%93WRKY40%20and%20HY5%E2%80%93ABI5%20modules%20regulate%20the%20expression%20of%20ABA-responsive%20genes%20in%20Arabidopsis&rft.jtitle=The%20New%20phytologist&rft.au=Wang,%20Tian-Jing&rft.date=2021-04-01&rft.volume=230&rft.issue=2&rft.spage=567&rft.epage=584&rft.pages=567-584&rft.issn=0028-646X&rft.eissn=1469-8137&rft_id=info:doi/10.1111/nph.17177&rft_dat=%3Cjstor_proqu%3E27013898%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2501878828&rft_id=info:pmid/33423315&rft_jstor_id=27013898&rfr_iscdi=true |