Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse
Purpose To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP). Methods Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2021-05, Vol.303 (5), p.1245-1253 |
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| container_title | Archives of gynecology and obstetrics |
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| creator | Lin, Te Ji, Yuting Zhao, Ying Xia, Zhijun |
| description | Purpose
To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP).
Methods
Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the expressions of COX-2/PGE2, Nrf2/GPx3, MMP2, TIMP1, collagen I and collagen III (
n
= 35, per group).
Results
Compared with control group, collagen fibers of the anterior vaginal wall were disorganized and discontinuous. Expressions of Nrf2, GPx3, TIMP1, collagen I and collagen III were found significantly lower in POP group (
P
|
| doi_str_mv | 10.1007/s00404-020-05913-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2476567670</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2513720451</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-6de272ee87962a2ae2fd6468cac025f4ae9a76bdd73172166fc861d4b287758d3</originalsourceid><addsrcrecordid>eNp9kU1PGzEQhq2qqITAH-ihstRLL1vGH2s7xypKoRJqOIDEzXJ2Z4OjzXprbz749ziEthIHTmPZz_uOZ15CPjP4zgD0ZQKQIAvgUEA5YaIwH8iIScEL0Ix9JCOYHM6g9Ck5S2kFwLgx6hM5FUKyUgo9IqvZvo-Ykg8dDQ2dzh8KTl1X09-x4ZdXt3tBfUeHR8yXA0YfIt26pe9cS3eubengU9pgOmh3YY0d3fnhkfbYbn1FQ1y6jvYxtK5PeE5OGtcmvHitY3L_c3Y3vS5u5le_pj9uikrocihUjVxzRKMnijvukDe1kspUrgJeNtLhxGm1qGstmOZMqaYyitVywY3WpanFmHw7-ubGf_LfBrv2qcK2dR2GTbJcalUqrTRk9OsbdBU2MQ-XqZIJzUHmMib8SFUxpBSxsX30axefLAN7SMIek7A5CfuShDVZ9OXVerNYY_1P8nf1GRBHIOWnbonxf-93bJ8B20uSUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2513720451</pqid></control><display><type>article</type><title>Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse</title><source>SpringerLink Journals - AutoHoldings</source><creator>Lin, Te ; Ji, Yuting ; Zhao, Ying ; Xia, Zhijun</creator><creatorcontrib>Lin, Te ; Ji, Yuting ; Zhao, Ying ; Xia, Zhijun</creatorcontrib><description>Purpose
To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP).
Methods
Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the expressions of COX-2/PGE2, Nrf2/GPx3, MMP2, TIMP1, collagen I and collagen III (
n
= 35, per group).
Results
Compared with control group, collagen fibers of the anterior vaginal wall were disorganized and discontinuous. Expressions of Nrf2, GPx3, TIMP1, collagen I and collagen III were found significantly lower in POP group (
P
< 0.05); while, expressions of COX-2, PGE2, and MMP2 were found significantly higher in POP group (
P
< 0.05). Statistically significant correlations of COX-2 and Nrf2/GPx3 were showed (
P
< 0.01).
Conclusion
We found that the interaction between inflammation and oxidative stress was closely related to the development of POP. This study demonstrates that COX-2 and Nrf2 pathways may be involved in pathogenesis of POP, as promising potential therapeutic targets and agents.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-020-05913-8</identifier><identifier>PMID: 33415437</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Collagen ; Endocrinology ; General Gynecology ; Gynecology ; Human Genetics ; Medicine ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Oxidative stress ; Pelvic organ prolapse ; Vagina</subject><ispartof>Archives of gynecology and obstetrics, 2021-05, Vol.303 (5), p.1245-1253</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021. corrected publication 2021</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021. corrected publication 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-6de272ee87962a2ae2fd6468cac025f4ae9a76bdd73172166fc861d4b287758d3</citedby><cites>FETCH-LOGICAL-c375t-6de272ee87962a2ae2fd6468cac025f4ae9a76bdd73172166fc861d4b287758d3</cites><orcidid>0000-0001-7387-1665 ; 0000-0002-5794-9327</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-020-05913-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-020-05913-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33415437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Te</creatorcontrib><creatorcontrib>Ji, Yuting</creatorcontrib><creatorcontrib>Zhao, Ying</creatorcontrib><creatorcontrib>Xia, Zhijun</creatorcontrib><title>Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP).
Methods
Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the expressions of COX-2/PGE2, Nrf2/GPx3, MMP2, TIMP1, collagen I and collagen III (
n
= 35, per group).
Results
Compared with control group, collagen fibers of the anterior vaginal wall were disorganized and discontinuous. Expressions of Nrf2, GPx3, TIMP1, collagen I and collagen III were found significantly lower in POP group (
P
< 0.05); while, expressions of COX-2, PGE2, and MMP2 were found significantly higher in POP group (
P
< 0.05). Statistically significant correlations of COX-2 and Nrf2/GPx3 were showed (
P
< 0.01).
Conclusion
We found that the interaction between inflammation and oxidative stress was closely related to the development of POP. This study demonstrates that COX-2 and Nrf2 pathways may be involved in pathogenesis of POP, as promising potential therapeutic targets and agents.</description><subject>Collagen</subject><subject>Endocrinology</subject><subject>General Gynecology</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Oxidative stress</subject><subject>Pelvic organ prolapse</subject><subject>Vagina</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU1PGzEQhq2qqITAH-ihstRLL1vGH2s7xypKoRJqOIDEzXJ2Z4OjzXprbz749ziEthIHTmPZz_uOZ15CPjP4zgD0ZQKQIAvgUEA5YaIwH8iIScEL0Ix9JCOYHM6g9Ck5S2kFwLgx6hM5FUKyUgo9IqvZvo-Ykg8dDQ2dzh8KTl1X09-x4ZdXt3tBfUeHR8yXA0YfIt26pe9cS3eubengU9pgOmh3YY0d3fnhkfbYbn1FQ1y6jvYxtK5PeE5OGtcmvHitY3L_c3Y3vS5u5le_pj9uikrocihUjVxzRKMnijvukDe1kspUrgJeNtLhxGm1qGstmOZMqaYyitVywY3WpanFmHw7-ubGf_LfBrv2qcK2dR2GTbJcalUqrTRk9OsbdBU2MQ-XqZIJzUHmMib8SFUxpBSxsX30axefLAN7SMIek7A5CfuShDVZ9OXVerNYY_1P8nf1GRBHIOWnbonxf-93bJ8B20uSUA</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Lin, Te</creator><creator>Ji, Yuting</creator><creator>Zhao, Ying</creator><creator>Xia, Zhijun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7387-1665</orcidid><orcidid>https://orcid.org/0000-0002-5794-9327</orcidid></search><sort><creationdate>20210501</creationdate><title>Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse</title><author>Lin, Te ; Ji, Yuting ; Zhao, Ying ; Xia, Zhijun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-6de272ee87962a2ae2fd6468cac025f4ae9a76bdd73172166fc861d4b287758d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Collagen</topic><topic>Endocrinology</topic><topic>General Gynecology</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Oxidative stress</topic><topic>Pelvic organ prolapse</topic><topic>Vagina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Te</creatorcontrib><creatorcontrib>Ji, Yuting</creatorcontrib><creatorcontrib>Zhao, Ying</creatorcontrib><creatorcontrib>Xia, Zhijun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Te</au><au>Ji, Yuting</au><au>Zhao, Ying</au><au>Xia, Zhijun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>303</volume><issue>5</issue><spage>1245</spage><epage>1253</epage><pages>1245-1253</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP).
Methods
Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the expressions of COX-2/PGE2, Nrf2/GPx3, MMP2, TIMP1, collagen I and collagen III (
n
= 35, per group).
Results
Compared with control group, collagen fibers of the anterior vaginal wall were disorganized and discontinuous. Expressions of Nrf2, GPx3, TIMP1, collagen I and collagen III were found significantly lower in POP group (
P
< 0.05); while, expressions of COX-2, PGE2, and MMP2 were found significantly higher in POP group (
P
< 0.05). Statistically significant correlations of COX-2 and Nrf2/GPx3 were showed (
P
< 0.01).
Conclusion
We found that the interaction between inflammation and oxidative stress was closely related to the development of POP. This study demonstrates that COX-2 and Nrf2 pathways may be involved in pathogenesis of POP, as promising potential therapeutic targets and agents.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33415437</pmid><doi>10.1007/s00404-020-05913-8</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7387-1665</orcidid><orcidid>https://orcid.org/0000-0002-5794-9327</orcidid></addata></record> |
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| ispartof | Archives of gynecology and obstetrics, 2021-05, Vol.303 (5), p.1245-1253 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Collagen Endocrinology General Gynecology Gynecology Human Genetics Medicine Medicine & Public Health Obstetrics/Perinatology/Midwifery Oxidative stress Pelvic organ prolapse Vagina |
| title | Expression of COX-2 and Nrf2/GPx3 in the anterior vaginal wall tissues of women with pelvic organ prolapse |
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