Fibroblast Growth Factor 9 Stimulates Neuronal Length Through NF-kB Signaling in Striatal Cell Huntington’s Disease Models
Proper development of neuronal cells is important for brain functions, and impairment of neuronal development may lead to neuronal disorders, implying that improvement in neuronal development may be a therapeutic direction for these diseases. Huntington’s disease (HD) is a neurodegenerative disease...
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| Veröffentlicht in: | Molecular neurobiology 2021-05, Vol.58 (5), p.2396-2406 |
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| creator | Yusuf, Issa Olakunle Chen, Hsiu-Mei Cheng, Pei-Hsun Chang, Chih-Yi Tsai, Shaw-Jenq Chuang, Jih-Ing Wu, Chia-Ching Huang, Bu-Miin Sun, H. Sunny Chen, Chuan-Mu Yang, Shang-Hsun |
| description | Proper development of neuronal cells is important for brain functions, and impairment of neuronal development may lead to neuronal disorders, implying that improvement in neuronal development may be a therapeutic direction for these diseases. Huntington’s disease (HD) is a neurodegenerative disease characterized by impairment of neuronal structures, ultimately leading to neuronal death and dysfunctions of the central nervous system. Based on previous studies, fibroblast growth factor 9 (FGF9) may provide neuroprotective functions in HD, and FGFs may enhance neuronal development and neurite outgrowth. However, whether FGF9 can provide neuronal protective functions through improvement of neuronal morphology in HD is still unclear. Here, we study the effects of FGF9 on neuronal length in HD and attempt to understand the related working mechanisms. Taking advantage of striatal cell lines from HD knock-in mice, we found that FGF9 increases total neuronal length and upregulates several structural and synaptic proteins under HD conditions. In addition, activation of nuclear factor kappa B (NF-kB) signaling by FGF9 was observed to be significant in HD cells, and blockage of NF-kB leads to suppression of these structural and synaptic proteins induced by FGF9, suggesting the involvement of NF-kB signaling in these effects of FGF9. Taken these results together, FGF9 may enhance total neuronal length through upregulation of NF-kB signaling, and this mechanism could serve as an important mechanism for neuroprotective functions of FGF9 in HD. |
| doi_str_mv | 10.1007/s12035-020-02220-w |
| format | Article |
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Sunny ; Chen, Chuan-Mu ; Yang, Shang-Hsun</creator><creatorcontrib>Yusuf, Issa Olakunle ; Chen, Hsiu-Mei ; Cheng, Pei-Hsun ; Chang, Chih-Yi ; Tsai, Shaw-Jenq ; Chuang, Jih-Ing ; Wu, Chia-Ching ; Huang, Bu-Miin ; Sun, H. Sunny ; Chen, Chuan-Mu ; Yang, Shang-Hsun</creatorcontrib><description>Proper development of neuronal cells is important for brain functions, and impairment of neuronal development may lead to neuronal disorders, implying that improvement in neuronal development may be a therapeutic direction for these diseases. Huntington’s disease (HD) is a neurodegenerative disease characterized by impairment of neuronal structures, ultimately leading to neuronal death and dysfunctions of the central nervous system. Based on previous studies, fibroblast growth factor 9 (FGF9) may provide neuroprotective functions in HD, and FGFs may enhance neuronal development and neurite outgrowth. However, whether FGF9 can provide neuronal protective functions through improvement of neuronal morphology in HD is still unclear. Here, we study the effects of FGF9 on neuronal length in HD and attempt to understand the related working mechanisms. Taking advantage of striatal cell lines from HD knock-in mice, we found that FGF9 increases total neuronal length and upregulates several structural and synaptic proteins under HD conditions. In addition, activation of nuclear factor kappa B (NF-kB) signaling by FGF9 was observed to be significant in HD cells, and blockage of NF-kB leads to suppression of these structural and synaptic proteins induced by FGF9, suggesting the involvement of NF-kB signaling in these effects of FGF9. Taken these results together, FGF9 may enhance total neuronal length through upregulation of NF-kB signaling, and this mechanism could serve as an important mechanism for neuroprotective functions of FGF9 in HD.</description><identifier>ISSN: 0893-7648</identifier><identifier>EISSN: 1559-1182</identifier><identifier>DOI: 10.1007/s12035-020-02220-w</identifier><identifier>PMID: 33421017</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Axonogenesis ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell culture ; Cell lines ; Central nervous system ; Disease ; Fibroblast growth factor 9 ; Fibroblast growth factor receptor 9 ; Fibroblasts ; Growth factors ; Huntington's disease ; Huntingtons disease ; Neostriatum ; Neurobiology ; Neurodegenerative diseases ; Neurology ; Neuroprotection ; Neurosciences ; NF-κB protein</subject><ispartof>Molecular neurobiology, 2021-05, Vol.58 (5), p.2396-2406</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-bd7057942539a386e940756756c0f6ac627195a0d92c321aab814b5d563779e33</citedby><cites>FETCH-LOGICAL-c419t-bd7057942539a386e940756756c0f6ac627195a0d92c321aab814b5d563779e33</cites><orcidid>0000-0002-1601-3653</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12035-020-02220-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12035-020-02220-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33421017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yusuf, Issa Olakunle</creatorcontrib><creatorcontrib>Chen, Hsiu-Mei</creatorcontrib><creatorcontrib>Cheng, Pei-Hsun</creatorcontrib><creatorcontrib>Chang, Chih-Yi</creatorcontrib><creatorcontrib>Tsai, Shaw-Jenq</creatorcontrib><creatorcontrib>Chuang, Jih-Ing</creatorcontrib><creatorcontrib>Wu, Chia-Ching</creatorcontrib><creatorcontrib>Huang, Bu-Miin</creatorcontrib><creatorcontrib>Sun, H. Sunny</creatorcontrib><creatorcontrib>Chen, Chuan-Mu</creatorcontrib><creatorcontrib>Yang, Shang-Hsun</creatorcontrib><title>Fibroblast Growth Factor 9 Stimulates Neuronal Length Through NF-kB Signaling in Striatal Cell Huntington’s Disease Models</title><title>Molecular neurobiology</title><addtitle>Mol Neurobiol</addtitle><addtitle>Mol Neurobiol</addtitle><description>Proper development of neuronal cells is important for brain functions, and impairment of neuronal development may lead to neuronal disorders, implying that improvement in neuronal development may be a therapeutic direction for these diseases. Huntington’s disease (HD) is a neurodegenerative disease characterized by impairment of neuronal structures, ultimately leading to neuronal death and dysfunctions of the central nervous system. Based on previous studies, fibroblast growth factor 9 (FGF9) may provide neuroprotective functions in HD, and FGFs may enhance neuronal development and neurite outgrowth. However, whether FGF9 can provide neuronal protective functions through improvement of neuronal morphology in HD is still unclear. Here, we study the effects of FGF9 on neuronal length in HD and attempt to understand the related working mechanisms. Taking advantage of striatal cell lines from HD knock-in mice, we found that FGF9 increases total neuronal length and upregulates several structural and synaptic proteins under HD conditions. In addition, activation of nuclear factor kappa B (NF-kB) signaling by FGF9 was observed to be significant in HD cells, and blockage of NF-kB leads to suppression of these structural and synaptic proteins induced by FGF9, suggesting the involvement of NF-kB signaling in these effects of FGF9. Taken these results together, FGF9 may enhance total neuronal length through upregulation of NF-kB signaling, and this mechanism could serve as an important mechanism for neuroprotective functions of FGF9 in HD.</description><subject>Axonogenesis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell lines</subject><subject>Central nervous system</subject><subject>Disease</subject><subject>Fibroblast growth factor 9</subject><subject>Fibroblast growth factor receptor 9</subject><subject>Fibroblasts</subject><subject>Growth factors</subject><subject>Huntington's disease</subject><subject>Huntingtons disease</subject><subject>Neostriatum</subject><subject>Neurobiology</subject><subject>Neurodegenerative diseases</subject><subject>Neurology</subject><subject>Neuroprotection</subject><subject>Neurosciences</subject><subject>NF-κB protein</subject><issn>0893-7648</issn><issn>1559-1182</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1OGzEUha2qqISUF-iistRNN1P8M7bHyxKagBToAlhbnhlnYjoZg-1RVKkLXoPX40l6IbRIXVTyn3S_c67tg9AHSr5QQtRRooxwURBGYDJYt2_QhAqhC0or9hZNSKV5oWRZ7aODlG4IUJSod2if8xJOVE3Qr7mvY6h7mzJexLDNazy3TQ4Ra3yZ_WbsbXYJX7gxhsH2eOmGDpirdQxjt8YX8-LHMb70HdT80GE_gCp6mwGdub7Hp-OQoZDD8Hj_kPCJT84mh89D6_r0Hu2tbJ_c4cs-Rdfzb1ez02L5fXE2-7osmpLqXNStIkLpkgmuLa-k0yVRQsJoyEraRjJFtbCk1azhjFpbV7SsRSskV0o7zqfo8873Noa70aVsNj41cD07uDAmw0olhSQVNJiiT_-gN2GM8DqgBKngB0v5RLEd1cSQUnQrcxv9xsafhhLzlI3ZZWMgG_OcjdmC6OOL9VhvXPtX8icMAPgOSFAaOhdfe__H9jeI9Zmb</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Yusuf, Issa Olakunle</creator><creator>Chen, Hsiu-Mei</creator><creator>Cheng, Pei-Hsun</creator><creator>Chang, Chih-Yi</creator><creator>Tsai, Shaw-Jenq</creator><creator>Chuang, Jih-Ing</creator><creator>Wu, Chia-Ching</creator><creator>Huang, Bu-Miin</creator><creator>Sun, H. 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Sunny</au><au>Chen, Chuan-Mu</au><au>Yang, Shang-Hsun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibroblast Growth Factor 9 Stimulates Neuronal Length Through NF-kB Signaling in Striatal Cell Huntington’s Disease Models</atitle><jtitle>Molecular neurobiology</jtitle><stitle>Mol Neurobiol</stitle><addtitle>Mol Neurobiol</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>58</volume><issue>5</issue><spage>2396</spage><epage>2406</epage><pages>2396-2406</pages><issn>0893-7648</issn><eissn>1559-1182</eissn><abstract>Proper development of neuronal cells is important for brain functions, and impairment of neuronal development may lead to neuronal disorders, implying that improvement in neuronal development may be a therapeutic direction for these diseases. Huntington’s disease (HD) is a neurodegenerative disease characterized by impairment of neuronal structures, ultimately leading to neuronal death and dysfunctions of the central nervous system. Based on previous studies, fibroblast growth factor 9 (FGF9) may provide neuroprotective functions in HD, and FGFs may enhance neuronal development and neurite outgrowth. However, whether FGF9 can provide neuronal protective functions through improvement of neuronal morphology in HD is still unclear. Here, we study the effects of FGF9 on neuronal length in HD and attempt to understand the related working mechanisms. Taking advantage of striatal cell lines from HD knock-in mice, we found that FGF9 increases total neuronal length and upregulates several structural and synaptic proteins under HD conditions. In addition, activation of nuclear factor kappa B (NF-kB) signaling by FGF9 was observed to be significant in HD cells, and blockage of NF-kB leads to suppression of these structural and synaptic proteins induced by FGF9, suggesting the involvement of NF-kB signaling in these effects of FGF9. Taken these results together, FGF9 may enhance total neuronal length through upregulation of NF-kB signaling, and this mechanism could serve as an important mechanism for neuroprotective functions of FGF9 in HD.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33421017</pmid><doi>10.1007/s12035-020-02220-w</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1601-3653</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Axonogenesis Biomedical and Life Sciences Biomedicine Cell Biology Cell culture Cell lines Central nervous system Disease Fibroblast growth factor 9 Fibroblast growth factor receptor 9 Fibroblasts Growth factors Huntington's disease Huntingtons disease Neostriatum Neurobiology Neurodegenerative diseases Neurology Neuroprotection Neurosciences NF-κB protein |
| title | Fibroblast Growth Factor 9 Stimulates Neuronal Length Through NF-kB Signaling in Striatal Cell Huntington’s Disease Models |
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