Proteomic analysis of hydrolytic proteases in excretory/secretory proteins from Trichinella spiralis intestinal infective larvae using zymography combined with shotgun LC-MS/MS approach

•Hydrolytic proteases in T. spiralis IIL ES proteins were analyzed by zymography.•Thirty-two T. spiralis proteases were identified by shotgun LC-MS/MS.•They are mainly serine proteinases, metalloproteinases and cysteine proteinases.•qPCR confirmed that 4 protease genes at IIL stage were up-regulated...

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Veröffentlicht in:	Acta tropica 2021-04, Vol.216, p.105825-105825, Article 105825
Hauptverfasser:	Ren, Hua Nan, Zhuo, Tong Xu, Bai, Sheng Jie, Bai, Ying, Sun, Xiang Yuan, Dan Liu, Ruo, Long, Shao Rong, Cui, Jing, Wang, Zhong Quan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Liquid ES proteins Helminth Proteins - genetics Helminth Proteins - metabolism Host-Parasite Interactions hydrolytic proteases intestinal infective larvae Intestines - parasitology Larva - genetics Larva - metabolism mass spectrometry Mice Mice, Inbred BALB C Muscle, Skeletal - parasitology Peptide Hydrolases - genetics Peptide Hydrolases - metabolism Polymerase Chain Reaction Proteome - analysis Proteomics - methods Real-Time Polymerase Chain Reaction RNA, Protozoan Specific Pathogen-Free Organisms Tandem Mass Spectrometry Trichinella spiralis Trichinella spiralis - enzymology Trichinella spiralis - genetics Trichinellosis - parasitology zymography
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