Nanogelation and Thermal Stabilization of Enzyme by Vitamin B6‑Bearing Polysaccharide as Biocrosslinker

Nanogels containing a protein (carbonic anhydrase, CA) were prepared by cross-linking CA and pyridoxal (vitamin B6)-bearing pullulan (PLPP) as a biocrosslinker via Schiff base formation. UV titration and high-performance liquid chromatography confirmed that CA was quantitatively complexed with PLPP...

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Veröffentlicht in:ACS biomaterials science & engineering 2019-11, Vol.5 (11), p.5752-5758
Hauptverfasser: Sasaki, Yoshihiro, Tsuchido, Yuji, Yoshimura, Takahiro, Akiyoshi, Kazunari
Format: Artikel
Sprache:eng
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