Study of expressions of miRNAs in the spikelets based on their spatial location on panicle in rice cultivars provided insight into their influence on grain development

Development of rice cultivars bearing numerous spikelets by breeding approach to increase the yearly production of rice to approximately 800 million metric tons to feed the ever increasing population of the world accompanies poor grain filling in the inferior spikelets preventing achievement of the...

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Veröffentlicht in:Plant physiology and biochemistry 2021-02, Vol.159, p.244-256
Hauptverfasser: Chandra, Tilak, Mishra, Sagarika, Panda, Binay Bhushan, Sahu, Gyanasri, Dash, Sushanta Kumar, Shaw, Birendra Prasad
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container_title Plant physiology and biochemistry
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creator Chandra, Tilak
Mishra, Sagarika
Panda, Binay Bhushan
Sahu, Gyanasri
Dash, Sushanta Kumar
Shaw, Birendra Prasad
description Development of rice cultivars bearing numerous spikelets by breeding approach to increase the yearly production of rice to approximately 800 million metric tons to feed the ever increasing population of the world accompanies poor grain filling in the inferior spikelets preventing achievement of the yield potential. As the initial stages of caryopses development are of much importance for grain filling, spatio-temporal expressions of the miRNAs were studied during these periods in the spikelets of a compact-panicle rice cultivar, Oryza sativa cv. Mahalaxmi, bearing numerous spikelets per panicle to understand the reason of poor grain filling at the level of the initial biochemical events. Differential expression of several known miRNAs between the superior and inferior spikelets suggested great difference in metabolism related to grain filling in the spikelets based on their spatial location on compact panicle. Expressions of five known and four novel miRNAs were validated by Northern. Their targets included the enzymes directly involved in starch biosynthesis like sucrose synthase, starch synthase and pullulanase, besides others. Spatio-temporal expression studies of these miRNAs in the spikelets of Mahalaxmi revealed a pattern of mostly a greater expression in the inferior spikelets compared with the superior ones concomitant with an inverse expression of the target genes, which was not observed in the lax-panicle cultivar Upahar. The study thus revealed that the grain filling in rice is greatly regulated by miRNAs, and these miRNAs or their target genes could be considered for biotechnological interventions for improving grain filling in the rice cultivars of interest. •The spikelets of compact-panicle rice show spatial difference in grain filling.•Expression of miRNAs differs in superior and inferior spikelets of compact-panicle.•Superior and inferior spikelets of lax-panicle show similar expression of a miRNA.•Many miRNAs expressed in spikelets targets starch biosynthesizing enzymes.•Inferior spikelets of compact-panicle show poor expression of grain filling enzymes.
doi_str_mv 10.1016/j.plaphy.2020.12.020
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subjects 5′RACE PCR
Compact-panicle
Edible Grain - genetics
Edible Grain - growth & development
Edible Grain - metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Lax-panicle
MicroRNAs - genetics
Oryza - genetics
Oryza - growth & development
Oryza sativa
Plant Breeding
Plant Proteins - genetics
Plant Proteins - metabolism
Pullulanase
Starch synthase
Sucrose synthase
title Study of expressions of miRNAs in the spikelets based on their spatial location on panicle in rice cultivars provided insight into their influence on grain development
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