Expression and functional role of IGFs during early pregnancy in placenta of water buffalo
Adequate vascularisation is a key factor for successful fetal development. We hypothesized that Insulin-Like Growth Factor (IGF) family members regulate angiogenesis along with promoting fetal development and growth. In this experiment, we determined the expression and functional role of IGF family...
Gespeichert in:
| Veröffentlicht in: | Theriogenology 2021-02, Vol.161, p.313-331 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 331 |
|---|---|
| container_issue | |
| container_start_page | 313 |
| container_title | Theriogenology |
| container_volume | 161 |
| creator | Pandey, Y. Pooja, A.R. Devi, H. Lakshmi Jalmeria, N. Singh Punetha, Meeti Kumar, S. Paul, A. Kumar, K. Sonawane, Arvind Samad, H.A. Singh, G. Bag, S. Sarkar, M. Chouhan, V.S. |
| description | Adequate vascularisation is a key factor for successful fetal development. We hypothesized that Insulin-Like Growth Factor (IGF) family members regulate angiogenesis along with promoting fetal development and growth. In this experiment, we determined the expression and functional role of IGF family in placental compartments (caruncle; CAR, cotyledon; COT) during different stages of early pregnancy in the water buffalo (Bubalus bubalis). Samples were collected from early pregnancy 1 (EP1, 28–45 days), early pregnancy 2 (EP2, 45–90 days), and third stage of estrous cycle (11–16 days), which was taken as control. In addition, the role of IGF1 on mRNA expression of vWF, StAR, CYP11A1, 3βHSD, PCNA, and BAX were elucidated in cultured trophoblast cells (TCC) obtained from EP2. Quantitative real-time PCR (q-PCR), westernblot, and immunohistochemistry were done to investigate the gene expression, protein expression, and localization of examined factors, and RIA was also done to assess progesterone (P4) concentration. Expression of IGFs, its receptors and binding proteins were found to be significantly higher (p |
| doi_str_mv | 10.1016/j.theriogenology.2020.12.013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2473899816</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0093691X20306452</els_id><sourcerecordid>2473899816</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-64bacc336945c5eb2e2b8915d84f74842515a95813205b3ec26720dcae5ba73c3</originalsourceid><addsrcrecordid>eNqNkMFqGzEQhkVJiJ00rxB06CGXdSXNrnYFvZQQp4ZALgmEXoRWO-vKrCVH2k3rt6-Mk0JvOQ3DfP8M8xHyhbMFZ1x-3SzGXxhdWKMPQ1jvF4KJPBILxuETmfOmVgUI4CdkzpiCQir-PCPnKW0YYyAlPyMzAKhBQTknP2__7CKm5IKnxne0n7wdc2MGGsOANPR0dbdMtJui82uKJg57mhNrb7zdU-fpbjAW_WgO6G8zYqTt1PdmCJ_JaS4JL9_qBXla3j7e_CjuH-5WN9_vCwuNHAtZtsZaAKnKylbYChRto3jVNWVfl00pKl4ZVTUcBKtaQCtkLVhnDVatqcHCBbk-7t3F8DJhGvXWJYvDYDyGKWlR1tAo1XCZ0W9H1MaQUsRe76LbmrjXnOmDXb3R_9vVB7uaC53t5vjV26Wp3WL3L_yuMwPLI4D531eHUSfr0FvsXEQ76i64j136C_bFlM0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2473899816</pqid></control><display><type>article</type><title>Expression and functional role of IGFs during early pregnancy in placenta of water buffalo</title><source>Access via ScienceDirect (Elsevier)</source><creator>Pandey, Y. ; Pooja, A.R. ; Devi, H. Lakshmi ; Jalmeria, N. Singh ; Punetha, Meeti ; Kumar, S. ; Paul, A. ; Kumar, K. ; Sonawane, Arvind ; Samad, H.A. ; Singh, G. ; Bag, S. ; Sarkar, M. ; Chouhan, V.S.</creator><creatorcontrib>Pandey, Y. ; Pooja, A.R. ; Devi, H. Lakshmi ; Jalmeria, N. Singh ; Punetha, Meeti ; Kumar, S. ; Paul, A. ; Kumar, K. ; Sonawane, Arvind ; Samad, H.A. ; Singh, G. ; Bag, S. ; Sarkar, M. ; Chouhan, V.S.</creatorcontrib><description>Adequate vascularisation is a key factor for successful fetal development. We hypothesized that Insulin-Like Growth Factor (IGF) family members regulate angiogenesis along with promoting fetal development and growth. In this experiment, we determined the expression and functional role of IGF family in placental compartments (caruncle; CAR, cotyledon; COT) during different stages of early pregnancy in the water buffalo (Bubalus bubalis). Samples were collected from early pregnancy 1 (EP1, 28–45 days), early pregnancy 2 (EP2, 45–90 days), and third stage of estrous cycle (11–16 days), which was taken as control. In addition, the role of IGF1 on mRNA expression of vWF, StAR, CYP11A1, 3βHSD, PCNA, and BAX were elucidated in cultured trophoblast cells (TCC) obtained from EP2. Quantitative real-time PCR (q-PCR), westernblot, and immunohistochemistry were done to investigate the gene expression, protein expression, and localization of examined factors, and RIA was also done to assess progesterone (P4) concentration. Expression of IGFs, its receptors and binding proteins were found to be significantly higher (p < 0.05) in both CAR and COT as compared to control during early pregnancy, except binding proteins IGFBP1, 3 and 4 which were significantly (p < 0.05) downregulated in COT with advancement of pregnancy. mRNA expression was consistent with the findings of immunoblotting and immunolocalization experiments. Trophoblasts cell culture (TCC) study showed a significant time and dose-dependent effect of IGF1 onsteroidogenic transcript, which was found to be maximum at 100 ng/ml that paralleled with P4 accretion in the media (p < 0.05). Further, IGF1 upregulated the transcripts of vWF, PCNA, and downregulated BAX at the same concentration (p < 0.05). Overall, our results demonstrated that the expression of IGFs is a site-specific phenomenon in placentome, which indicates autocrine/paracrine and endocrine function. Our in-vitro finding support that IGF1 plays a critical role in placental development by promoting angiogenesis, steroid synthesis, and cell proliferation during early pregnancy.
•IGF family and their receptors are expressed in caruncle and cotyledon in a stage specific manner in water buffalo.•IGF1 acts in a synergistic manner to stimulate progesterone (P4) secretion in cultured bubaline trophoblast cells.•IGF1 stimulates trophoblast cell steroidogenesis and angiogenesis.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2020.12.013</identifier><identifier>PMID: 33373934</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Angiogenesis ; Caruncle ; Cotyledon ; Early pregnancy ; Trophoblast</subject><ispartof>Theriogenology, 2021-02, Vol.161, p.313-331</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-64bacc336945c5eb2e2b8915d84f74842515a95813205b3ec26720dcae5ba73c3</citedby><cites>FETCH-LOGICAL-c386t-64bacc336945c5eb2e2b8915d84f74842515a95813205b3ec26720dcae5ba73c3</cites><orcidid>0000-0002-0365-0653</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2020.12.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33373934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pandey, Y.</creatorcontrib><creatorcontrib>Pooja, A.R.</creatorcontrib><creatorcontrib>Devi, H. Lakshmi</creatorcontrib><creatorcontrib>Jalmeria, N. Singh</creatorcontrib><creatorcontrib>Punetha, Meeti</creatorcontrib><creatorcontrib>Kumar, S.</creatorcontrib><creatorcontrib>Paul, A.</creatorcontrib><creatorcontrib>Kumar, K.</creatorcontrib><creatorcontrib>Sonawane, Arvind</creatorcontrib><creatorcontrib>Samad, H.A.</creatorcontrib><creatorcontrib>Singh, G.</creatorcontrib><creatorcontrib>Bag, S.</creatorcontrib><creatorcontrib>Sarkar, M.</creatorcontrib><creatorcontrib>Chouhan, V.S.</creatorcontrib><title>Expression and functional role of IGFs during early pregnancy in placenta of water buffalo</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Adequate vascularisation is a key factor for successful fetal development. We hypothesized that Insulin-Like Growth Factor (IGF) family members regulate angiogenesis along with promoting fetal development and growth. In this experiment, we determined the expression and functional role of IGF family in placental compartments (caruncle; CAR, cotyledon; COT) during different stages of early pregnancy in the water buffalo (Bubalus bubalis). Samples were collected from early pregnancy 1 (EP1, 28–45 days), early pregnancy 2 (EP2, 45–90 days), and third stage of estrous cycle (11–16 days), which was taken as control. In addition, the role of IGF1 on mRNA expression of vWF, StAR, CYP11A1, 3βHSD, PCNA, and BAX were elucidated in cultured trophoblast cells (TCC) obtained from EP2. Quantitative real-time PCR (q-PCR), westernblot, and immunohistochemistry were done to investigate the gene expression, protein expression, and localization of examined factors, and RIA was also done to assess progesterone (P4) concentration. Expression of IGFs, its receptors and binding proteins were found to be significantly higher (p < 0.05) in both CAR and COT as compared to control during early pregnancy, except binding proteins IGFBP1, 3 and 4 which were significantly (p < 0.05) downregulated in COT with advancement of pregnancy. mRNA expression was consistent with the findings of immunoblotting and immunolocalization experiments. Trophoblasts cell culture (TCC) study showed a significant time and dose-dependent effect of IGF1 onsteroidogenic transcript, which was found to be maximum at 100 ng/ml that paralleled with P4 accretion in the media (p < 0.05). Further, IGF1 upregulated the transcripts of vWF, PCNA, and downregulated BAX at the same concentration (p < 0.05). Overall, our results demonstrated that the expression of IGFs is a site-specific phenomenon in placentome, which indicates autocrine/paracrine and endocrine function. Our in-vitro finding support that IGF1 plays a critical role in placental development by promoting angiogenesis, steroid synthesis, and cell proliferation during early pregnancy.
•IGF family and their receptors are expressed in caruncle and cotyledon in a stage specific manner in water buffalo.•IGF1 acts in a synergistic manner to stimulate progesterone (P4) secretion in cultured bubaline trophoblast cells.•IGF1 stimulates trophoblast cell steroidogenesis and angiogenesis.</description><subject>Angiogenesis</subject><subject>Caruncle</subject><subject>Cotyledon</subject><subject>Early pregnancy</subject><subject>Trophoblast</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNkMFqGzEQhkVJiJ00rxB06CGXdSXNrnYFvZQQp4ZALgmEXoRWO-vKrCVH2k3rt6-Mk0JvOQ3DfP8M8xHyhbMFZ1x-3SzGXxhdWKMPQ1jvF4KJPBILxuETmfOmVgUI4CdkzpiCQir-PCPnKW0YYyAlPyMzAKhBQTknP2__7CKm5IKnxne0n7wdc2MGGsOANPR0dbdMtJui82uKJg57mhNrb7zdU-fpbjAW_WgO6G8zYqTt1PdmCJ_JaS4JL9_qBXla3j7e_CjuH-5WN9_vCwuNHAtZtsZaAKnKylbYChRto3jVNWVfl00pKl4ZVTUcBKtaQCtkLVhnDVatqcHCBbk-7t3F8DJhGvXWJYvDYDyGKWlR1tAo1XCZ0W9H1MaQUsRe76LbmrjXnOmDXb3R_9vVB7uaC53t5vjV26Wp3WL3L_yuMwPLI4D531eHUSfr0FvsXEQ76i64j136C_bFlM0</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Pandey, Y.</creator><creator>Pooja, A.R.</creator><creator>Devi, H. Lakshmi</creator><creator>Jalmeria, N. Singh</creator><creator>Punetha, Meeti</creator><creator>Kumar, S.</creator><creator>Paul, A.</creator><creator>Kumar, K.</creator><creator>Sonawane, Arvind</creator><creator>Samad, H.A.</creator><creator>Singh, G.</creator><creator>Bag, S.</creator><creator>Sarkar, M.</creator><creator>Chouhan, V.S.</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0365-0653</orcidid></search><sort><creationdate>202102</creationdate><title>Expression and functional role of IGFs during early pregnancy in placenta of water buffalo</title><author>Pandey, Y. ; Pooja, A.R. ; Devi, H. Lakshmi ; Jalmeria, N. Singh ; Punetha, Meeti ; Kumar, S. ; Paul, A. ; Kumar, K. ; Sonawane, Arvind ; Samad, H.A. ; Singh, G. ; Bag, S. ; Sarkar, M. ; Chouhan, V.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-64bacc336945c5eb2e2b8915d84f74842515a95813205b3ec26720dcae5ba73c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Angiogenesis</topic><topic>Caruncle</topic><topic>Cotyledon</topic><topic>Early pregnancy</topic><topic>Trophoblast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pandey, Y.</creatorcontrib><creatorcontrib>Pooja, A.R.</creatorcontrib><creatorcontrib>Devi, H. Lakshmi</creatorcontrib><creatorcontrib>Jalmeria, N. Singh</creatorcontrib><creatorcontrib>Punetha, Meeti</creatorcontrib><creatorcontrib>Kumar, S.</creatorcontrib><creatorcontrib>Paul, A.</creatorcontrib><creatorcontrib>Kumar, K.</creatorcontrib><creatorcontrib>Sonawane, Arvind</creatorcontrib><creatorcontrib>Samad, H.A.</creatorcontrib><creatorcontrib>Singh, G.</creatorcontrib><creatorcontrib>Bag, S.</creatorcontrib><creatorcontrib>Sarkar, M.</creatorcontrib><creatorcontrib>Chouhan, V.S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pandey, Y.</au><au>Pooja, A.R.</au><au>Devi, H. Lakshmi</au><au>Jalmeria, N. Singh</au><au>Punetha, Meeti</au><au>Kumar, S.</au><au>Paul, A.</au><au>Kumar, K.</au><au>Sonawane, Arvind</au><au>Samad, H.A.</au><au>Singh, G.</au><au>Bag, S.</au><au>Sarkar, M.</au><au>Chouhan, V.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and functional role of IGFs during early pregnancy in placenta of water buffalo</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2021-02</date><risdate>2021</risdate><volume>161</volume><spage>313</spage><epage>331</epage><pages>313-331</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Adequate vascularisation is a key factor for successful fetal development. We hypothesized that Insulin-Like Growth Factor (IGF) family members regulate angiogenesis along with promoting fetal development and growth. In this experiment, we determined the expression and functional role of IGF family in placental compartments (caruncle; CAR, cotyledon; COT) during different stages of early pregnancy in the water buffalo (Bubalus bubalis). Samples were collected from early pregnancy 1 (EP1, 28–45 days), early pregnancy 2 (EP2, 45–90 days), and third stage of estrous cycle (11–16 days), which was taken as control. In addition, the role of IGF1 on mRNA expression of vWF, StAR, CYP11A1, 3βHSD, PCNA, and BAX were elucidated in cultured trophoblast cells (TCC) obtained from EP2. Quantitative real-time PCR (q-PCR), westernblot, and immunohistochemistry were done to investigate the gene expression, protein expression, and localization of examined factors, and RIA was also done to assess progesterone (P4) concentration. Expression of IGFs, its receptors and binding proteins were found to be significantly higher (p < 0.05) in both CAR and COT as compared to control during early pregnancy, except binding proteins IGFBP1, 3 and 4 which were significantly (p < 0.05) downregulated in COT with advancement of pregnancy. mRNA expression was consistent with the findings of immunoblotting and immunolocalization experiments. Trophoblasts cell culture (TCC) study showed a significant time and dose-dependent effect of IGF1 onsteroidogenic transcript, which was found to be maximum at 100 ng/ml that paralleled with P4 accretion in the media (p < 0.05). Further, IGF1 upregulated the transcripts of vWF, PCNA, and downregulated BAX at the same concentration (p < 0.05). Overall, our results demonstrated that the expression of IGFs is a site-specific phenomenon in placentome, which indicates autocrine/paracrine and endocrine function. Our in-vitro finding support that IGF1 plays a critical role in placental development by promoting angiogenesis, steroid synthesis, and cell proliferation during early pregnancy.
•IGF family and their receptors are expressed in caruncle and cotyledon in a stage specific manner in water buffalo.•IGF1 acts in a synergistic manner to stimulate progesterone (P4) secretion in cultured bubaline trophoblast cells.•IGF1 stimulates trophoblast cell steroidogenesis and angiogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33373934</pmid><doi>10.1016/j.theriogenology.2020.12.013</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-0365-0653</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-691X |
| ispartof | Theriogenology, 2021-02, Vol.161, p.313-331 |
| issn | 0093-691X 1879-3231 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2473899816 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Angiogenesis Caruncle Cotyledon Early pregnancy Trophoblast |
| title | Expression and functional role of IGFs during early pregnancy in placenta of water buffalo |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T21%3A44%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20and%20functional%20role%20of%20IGFs%20during%20early%20pregnancy%20in%20placenta%20of%20water%20buffalo&rft.jtitle=Theriogenology&rft.au=Pandey,%20Y.&rft.date=2021-02&rft.volume=161&rft.spage=313&rft.epage=331&rft.pages=313-331&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/j.theriogenology.2020.12.013&rft_dat=%3Cproquest_cross%3E2473899816%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2473899816&rft_id=info:pmid/33373934&rft_els_id=S0093691X20306452&rfr_iscdi=true |