Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes
We demonstrate the application of four covalent probes based on anomerically pure d-galactosamine and d-glucosamine scaffolds for the profiling of Haemophilus influenzae strain R2866. The probes have been used successfully for the labelling of target proteins not only in cell lysates, but also in in...
Gespeichert in:
| Veröffentlicht in: | Organic & biomolecular chemistry 2021-01, Vol.19 (2), p.476-485 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 485 |
|---|---|
| container_issue | 2 |
| container_start_page | 476 |
| container_title | Organic & biomolecular chemistry |
| container_volume | 19 |
| creator | Metier, Camille Dow, Jennifer Wootton, Hayley Lynham, Steven Wren, Brendan Wagner, Gerd K |
| description | We demonstrate the application of four covalent probes based on anomerically pure d-galactosamine and d-glucosamine scaffolds for the profiling of Haemophilus influenzae strain R2866. The probes have been used successfully for the labelling of target proteins not only in cell lysates, but also in intact cells. Differences in the labelling patterns between lysates and intact cells indicate that the probes can penetrate into the periplasm, but not the cytoplasm of H. influenzae. Analysis of selected target proteins by LC-MS/MS suggests predominant labelling of nucleotide-binding proteins, including several known antibacterial drug targets. Our protocols will aid the identification of molecular determinants of bacterial pathogenicity in Haemophilus influenzae and other bacterial pathogens. |
| doi_str_mv | 10.1039/d0ob01971b |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2473419115</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2479448045</sourcerecordid><originalsourceid>FETCH-LOGICAL-c310t-880c4dccc7308af5e1fab1a9321bfd642f54a642355cb453fc6dd731cdd234453</originalsourceid><addsrcrecordid>eNpdkEtLAzEUhYMotlY3_gAJuBFhNJlkXktbHxWEiii4G_K0KTNJTWaU-uudaWsXrs7h8nHu4QBwitEVRqS4lshxhIsM8z0wxDTLIpSQYn_nYzQARyEsUA-l9BAMCCFJQmI8BO_P3mlTGfsBnYZTpmq3nJuqDdBYXbXK_jAFQ-OZsfAlztMUfptmDgXz3M1X0rNGRZwFJaFwX6xStoFL77gKx-BAsyqok62OwNv93etkGj3NHh4nN0-RIBg1UZ4jQaUQIiMoZzpRWDOOWdGV41qmNNYJZZ10fQWnCdEilTIjWEgZE9odRuBik9u9_WxVaMraBKGqilnl2lDGNCMUFxj36Pk_dOFab7t2PVVQmqN14OWGEt6F4JUul97UzK9KjMp-7_IWzcbrvccdfLaNbHmt5A79G5j8An47ekc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2479448045</pqid></control><display><type>article</type><title>Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes</title><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Metier, Camille ; Dow, Jennifer ; Wootton, Hayley ; Lynham, Steven ; Wren, Brendan ; Wagner, Gerd K</creator><creatorcontrib>Metier, Camille ; Dow, Jennifer ; Wootton, Hayley ; Lynham, Steven ; Wren, Brendan ; Wagner, Gerd K</creatorcontrib><description>We demonstrate the application of four covalent probes based on anomerically pure d-galactosamine and d-glucosamine scaffolds for the profiling of Haemophilus influenzae strain R2866. The probes have been used successfully for the labelling of target proteins not only in cell lysates, but also in intact cells. Differences in the labelling patterns between lysates and intact cells indicate that the probes can penetrate into the periplasm, but not the cytoplasm of H. influenzae. Analysis of selected target proteins by LC-MS/MS suggests predominant labelling of nucleotide-binding proteins, including several known antibacterial drug targets. Our protocols will aid the identification of molecular determinants of bacterial pathogenicity in Haemophilus influenzae and other bacterial pathogens.</description><identifier>ISSN: 1477-0520</identifier><identifier>EISSN: 1477-0539</identifier><identifier>DOI: 10.1039/d0ob01971b</identifier><identifier>PMID: 33355321</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Antiinfectives and antibacterials ; Carbohydrates ; Cytoplasm ; D-Galactosamine ; Glucosamine ; Haemophilus influenzae ; Labeling ; Lysates ; Nucleotides ; Pathogenicity ; Pathogens ; Periplasm ; Probes ; Proteins ; Therapeutic targets</subject><ispartof>Organic & biomolecular chemistry, 2021-01, Vol.19 (2), p.476-485</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c310t-880c4dccc7308af5e1fab1a9321bfd642f54a642355cb453fc6dd731cdd234453</cites><orcidid>0000-0003-1086-2301</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33355321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Metier, Camille</creatorcontrib><creatorcontrib>Dow, Jennifer</creatorcontrib><creatorcontrib>Wootton, Hayley</creatorcontrib><creatorcontrib>Lynham, Steven</creatorcontrib><creatorcontrib>Wren, Brendan</creatorcontrib><creatorcontrib>Wagner, Gerd K</creatorcontrib><title>Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes</title><title>Organic & biomolecular chemistry</title><addtitle>Org Biomol Chem</addtitle><description>We demonstrate the application of four covalent probes based on anomerically pure d-galactosamine and d-glucosamine scaffolds for the profiling of Haemophilus influenzae strain R2866. The probes have been used successfully for the labelling of target proteins not only in cell lysates, but also in intact cells. Differences in the labelling patterns between lysates and intact cells indicate that the probes can penetrate into the periplasm, but not the cytoplasm of H. influenzae. Analysis of selected target proteins by LC-MS/MS suggests predominant labelling of nucleotide-binding proteins, including several known antibacterial drug targets. Our protocols will aid the identification of molecular determinants of bacterial pathogenicity in Haemophilus influenzae and other bacterial pathogens.</description><subject>Antiinfectives and antibacterials</subject><subject>Carbohydrates</subject><subject>Cytoplasm</subject><subject>D-Galactosamine</subject><subject>Glucosamine</subject><subject>Haemophilus influenzae</subject><subject>Labeling</subject><subject>Lysates</subject><subject>Nucleotides</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Periplasm</subject><subject>Probes</subject><subject>Proteins</subject><subject>Therapeutic targets</subject><issn>1477-0520</issn><issn>1477-0539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkEtLAzEUhYMotlY3_gAJuBFhNJlkXktbHxWEiii4G_K0KTNJTWaU-uudaWsXrs7h8nHu4QBwitEVRqS4lshxhIsM8z0wxDTLIpSQYn_nYzQARyEsUA-l9BAMCCFJQmI8BO_P3mlTGfsBnYZTpmq3nJuqDdBYXbXK_jAFQ-OZsfAlztMUfptmDgXz3M1X0rNGRZwFJaFwX6xStoFL77gKx-BAsyqok62OwNv93etkGj3NHh4nN0-RIBg1UZ4jQaUQIiMoZzpRWDOOWdGV41qmNNYJZZ10fQWnCdEilTIjWEgZE9odRuBik9u9_WxVaMraBKGqilnl2lDGNCMUFxj36Pk_dOFab7t2PVVQmqN14OWGEt6F4JUul97UzK9KjMp-7_IWzcbrvccdfLaNbHmt5A79G5j8An47ekc</recordid><startdate>20210121</startdate><enddate>20210121</enddate><creator>Metier, Camille</creator><creator>Dow, Jennifer</creator><creator>Wootton, Hayley</creator><creator>Lynham, Steven</creator><creator>Wren, Brendan</creator><creator>Wagner, Gerd K</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1086-2301</orcidid></search><sort><creationdate>20210121</creationdate><title>Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes</title><author>Metier, Camille ; Dow, Jennifer ; Wootton, Hayley ; Lynham, Steven ; Wren, Brendan ; Wagner, Gerd K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-880c4dccc7308af5e1fab1a9321bfd642f54a642355cb453fc6dd731cdd234453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Carbohydrates</topic><topic>Cytoplasm</topic><topic>D-Galactosamine</topic><topic>Glucosamine</topic><topic>Haemophilus influenzae</topic><topic>Labeling</topic><topic>Lysates</topic><topic>Nucleotides</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Periplasm</topic><topic>Probes</topic><topic>Proteins</topic><topic>Therapeutic targets</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metier, Camille</creatorcontrib><creatorcontrib>Dow, Jennifer</creatorcontrib><creatorcontrib>Wootton, Hayley</creatorcontrib><creatorcontrib>Lynham, Steven</creatorcontrib><creatorcontrib>Wren, Brendan</creatorcontrib><creatorcontrib>Wagner, Gerd K</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Organic & biomolecular chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metier, Camille</au><au>Dow, Jennifer</au><au>Wootton, Hayley</au><au>Lynham, Steven</au><au>Wren, Brendan</au><au>Wagner, Gerd K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes</atitle><jtitle>Organic & biomolecular chemistry</jtitle><addtitle>Org Biomol Chem</addtitle><date>2021-01-21</date><risdate>2021</risdate><volume>19</volume><issue>2</issue><spage>476</spage><epage>485</epage><pages>476-485</pages><issn>1477-0520</issn><eissn>1477-0539</eissn><abstract>We demonstrate the application of four covalent probes based on anomerically pure d-galactosamine and d-glucosamine scaffolds for the profiling of Haemophilus influenzae strain R2866. The probes have been used successfully for the labelling of target proteins not only in cell lysates, but also in intact cells. Differences in the labelling patterns between lysates and intact cells indicate that the probes can penetrate into the periplasm, but not the cytoplasm of H. influenzae. Analysis of selected target proteins by LC-MS/MS suggests predominant labelling of nucleotide-binding proteins, including several known antibacterial drug targets. Our protocols will aid the identification of molecular determinants of bacterial pathogenicity in Haemophilus influenzae and other bacterial pathogens.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>33355321</pmid><doi>10.1039/d0ob01971b</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1086-2301</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1477-0520 |
| ispartof | Organic & biomolecular chemistry, 2021-01, Vol.19 (2), p.476-485 |
| issn | 1477-0520 1477-0539 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2473419115 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Antiinfectives and antibacterials Carbohydrates Cytoplasm D-Galactosamine Glucosamine Haemophilus influenzae Labeling Lysates Nucleotides Pathogenicity Pathogens Periplasm Probes Proteins Therapeutic targets |
| title | Profiling of Haemophilus influenzae strain R2866 with carbohydrate-based covalent probes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T16%3A41%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Profiling%20of%20Haemophilus%20influenzae%20strain%20R2866%20with%20carbohydrate-based%20covalent%20probes&rft.jtitle=Organic%20&%20biomolecular%20chemistry&rft.au=Metier,%20Camille&rft.date=2021-01-21&rft.volume=19&rft.issue=2&rft.spage=476&rft.epage=485&rft.pages=476-485&rft.issn=1477-0520&rft.eissn=1477-0539&rft_id=info:doi/10.1039/d0ob01971b&rft_dat=%3Cproquest_cross%3E2479448045%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2479448045&rft_id=info:pmid/33355321&rfr_iscdi=true |