Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Classic depletion-reconstitution experiments indicate that galectin-3 is a required splicing factor in nuclear extracts. The mechanism of incorporation of galectin-3 into the splicing pathway is addressed in this paper. Sedimentation of HeLa cell nuclear extracts on 12%-32% glycerol gradients yields...

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Veröffentlicht in:	Journal of visualized experiments 2020-12 (166)
Hauptverfasser:	Voss, Patricia G, Haudek, Kevin C, Patterson, Ronald J, Wang, John L
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Nucleus - metabolism Galectin 3 - genetics Galectin 3 - metabolism HeLa Cells Humans Microspheres Peptides - metabolism Ribonucleoprotein, U1 Small Nuclear - genetics Ribonucleoprotein, U1 Small Nuclear - metabolism RNA Precursors - metabolism RNA Splicing - genetics
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