Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core
Fluorescence imaging using the over-thousand-nanometer (OTN) near-infrared (NIR) light is an emerging method for an in vivo imaging analysis of deep tissues without physical sectioning. Polymer micelle nanoparticles (PNPs) composed of organic polymers encapsulating an OTN-NIR fluorescent dye, IR-106...
Gespeichert in:
| Veröffentlicht in: | Analytical Sciences 2021/03/10, Vol.37(3), pp.485-490 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 490 |
|---|---|
| container_issue | 3 |
| container_start_page | 485 |
| container_title | Analytical Sciences |
| container_volume | 37 |
| creator | UMEZAWA, Masakazu HARUKI, Mae YOSHIDA, Moe KAMIMURA, Masao SOGA, Kohei |
| description | Fluorescence imaging using the over-thousand-nanometer (OTN) near-infrared (NIR) light is an emerging method for an in vivo imaging analysis of deep tissues without physical sectioning. Polymer micelle nanoparticles (PNPs) composed of organic polymers encapsulating an OTN-NIR fluorescent dye, IR-1061, in their hydrophobic core are expected to be biocompatible probes. Because IR-1061 quickly quenches due to the vibration of polar hydroxyl bonding in its surroundings, the influence of hydroxyl ions should be minimized. Herein, we investigated the effect of the hydrogen ion concentration during the preparation process using IR-1061 and an organic polymer, poly(ethylene glycol)-block-polystyrene (PEG-b-PSt), on the emission properties of the obtained OTN-PNPs. The OTN-PNP has a hydrodynamic diameter of 20 – 30 nm and emits 1110-nm fluorescence that is applicable to angiography. The loading efficiency of IR-1061 in the OTN-PNPs increased when prepared in an aqueous solution with a low hydroxyl ion concentration. In this solution (pH 3.0), highly emissive OTN-PNPs was obtained with IR-1061 at lower nominal concentrations. Decreasing the hydroxyl ion concentration during the preparation process yields highly emissive OTN-PNPs, which may improve the in vivo imaging analysis of biological phenomena in deep tissues. |
| doi_str_mv | 10.2116/analsci.20SCP09 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2471539340</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2471539340</sourcerecordid><originalsourceid>FETCH-LOGICAL-c559t-82fe50a24bdb6777da5198329e0ed2aa5fe2dbc0733ff5c5b3af42d235f497ed3</originalsourceid><addsrcrecordid>eNp1kUuP0zAUhS0EYsrAmh2yxIZNZpzrOI8l6nRmKhWmErC2HOe6kyqxi52C8iP4z-PQUCQkNn7d7xz7-hDyNmVXkKb5tbKqC7q9AvZluWXVM7JIeVYmAFn-nCxYlbIk5xm7IK9C2DOWQgnwklxwzjOooFiQXytjUA-BOkO33mkMobU7erinztJV38ZtXKztgDa0wzhhDz_QJyljjNqefkblk7U1Xnls6G13dB6DRqtxQm9GTDZONbG0dd3Yo6efWo1dh_RnOzz-PgzD6NEiXUbla_LCxIbwzTxfkm-3q6_L-2TzcLdeftwkWohqSEowKJiCrG7qvCiKRom0KjlUyLABpYRBaGrNCs6NEVrUXJkMGuDCZFWBDb8kH06-B---HzEMMnY6vUtZdMcgIStSwav4cxF9_w-6d0c_fbsEwUrGAHIeqesTpb0LwaORB9_2yo8yZXJKSs5JyTmpqHg3-x7rHpsz_yeaCLATEGLJ7tD_vfj_nquTZB8GtcOzp_JDqzs887yQfBpm3bmuH5WXaPkTWOG6yA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2508002263</pqid></control><display><type>article</type><title>Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core</title><source>J-STAGE Free</source><source>MEDLINE</source><source>SpringerLink Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Open Access Titles of Japan</source><source>Free Full-Text Journals in Chemistry</source><creator>UMEZAWA, Masakazu ; HARUKI, Mae ; YOSHIDA, Moe ; KAMIMURA, Masao ; SOGA, Kohei</creator><creatorcontrib>UMEZAWA, Masakazu ; HARUKI, Mae ; YOSHIDA, Moe ; KAMIMURA, Masao ; SOGA, Kohei</creatorcontrib><description>Fluorescence imaging using the over-thousand-nanometer (OTN) near-infrared (NIR) light is an emerging method for an in vivo imaging analysis of deep tissues without physical sectioning. Polymer micelle nanoparticles (PNPs) composed of organic polymers encapsulating an OTN-NIR fluorescent dye, IR-1061, in their hydrophobic core are expected to be biocompatible probes. Because IR-1061 quickly quenches due to the vibration of polar hydroxyl bonding in its surroundings, the influence of hydroxyl ions should be minimized. Herein, we investigated the effect of the hydrogen ion concentration during the preparation process using IR-1061 and an organic polymer, poly(ethylene glycol)-block-polystyrene (PEG-b-PSt), on the emission properties of the obtained OTN-PNPs. The OTN-PNP has a hydrodynamic diameter of 20 – 30 nm and emits 1110-nm fluorescence that is applicable to angiography. The loading efficiency of IR-1061 in the OTN-PNPs increased when prepared in an aqueous solution with a low hydroxyl ion concentration. In this solution (pH 3.0), highly emissive OTN-PNPs was obtained with IR-1061 at lower nominal concentrations. Decreasing the hydroxyl ion concentration during the preparation process yields highly emissive OTN-PNPs, which may improve the in vivo imaging analysis of biological phenomena in deep tissues.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.20SCP09</identifier><identifier>PMID: 33342927</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Angiography ; Aqueous solutions ; Biocompatibility ; Biological analysis ; Chemistry ; Dyes ; Emission ; Emissions ; Fluorescence ; fluorescence bioimaging ; Fluorescent dyes ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Hydrogen ion concentration ; Hydrogen ions ; Hydrophobicity ; Hydroxyl ions ; I.R. radiation ; Imaging ; In vivo methods and tests ; Infrared Rays ; Ion concentration ; Ions ; Micelles ; Molecular Structure ; Nanoparticles ; Near infrared ; Near infrared radiation ; Optical Imaging ; organic dye ; Particle Size ; pH effects ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; polymer micelle ; Polymers ; Polystyrene ; Polystyrene resins ; Polystyrenes - chemistry ; Sectioning ; Surface Properties</subject><ispartof>Analytical Sciences, 2021/03/10, Vol.37(3), pp.485-490</ispartof><rights>2021 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2021</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-82fe50a24bdb6777da5198329e0ed2aa5fe2dbc0733ff5c5b3af42d235f497ed3</citedby><cites>FETCH-LOGICAL-c559t-82fe50a24bdb6777da5198329e0ed2aa5fe2dbc0733ff5c5b3af42d235f497ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.20SCP09$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.20SCP09$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,1883,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33342927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>UMEZAWA, Masakazu</creatorcontrib><creatorcontrib>HARUKI, Mae</creatorcontrib><creatorcontrib>YOSHIDA, Moe</creatorcontrib><creatorcontrib>KAMIMURA, Masao</creatorcontrib><creatorcontrib>SOGA, Kohei</creatorcontrib><title>Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>Fluorescence imaging using the over-thousand-nanometer (OTN) near-infrared (NIR) light is an emerging method for an in vivo imaging analysis of deep tissues without physical sectioning. Polymer micelle nanoparticles (PNPs) composed of organic polymers encapsulating an OTN-NIR fluorescent dye, IR-1061, in their hydrophobic core are expected to be biocompatible probes. Because IR-1061 quickly quenches due to the vibration of polar hydroxyl bonding in its surroundings, the influence of hydroxyl ions should be minimized. Herein, we investigated the effect of the hydrogen ion concentration during the preparation process using IR-1061 and an organic polymer, poly(ethylene glycol)-block-polystyrene (PEG-b-PSt), on the emission properties of the obtained OTN-PNPs. The OTN-PNP has a hydrodynamic diameter of 20 – 30 nm and emits 1110-nm fluorescence that is applicable to angiography. The loading efficiency of IR-1061 in the OTN-PNPs increased when prepared in an aqueous solution with a low hydroxyl ion concentration. In this solution (pH 3.0), highly emissive OTN-PNPs was obtained with IR-1061 at lower nominal concentrations. Decreasing the hydroxyl ion concentration during the preparation process yields highly emissive OTN-PNPs, which may improve the in vivo imaging analysis of biological phenomena in deep tissues.</description><subject>Analytical Chemistry</subject><subject>Angiography</subject><subject>Aqueous solutions</subject><subject>Biocompatibility</subject><subject>Biological analysis</subject><subject>Chemistry</subject><subject>Dyes</subject><subject>Emission</subject><subject>Emissions</subject><subject>Fluorescence</subject><subject>fluorescence bioimaging</subject><subject>Fluorescent dyes</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Hydrogen ion concentration</subject><subject>Hydrogen ions</subject><subject>Hydrophobicity</subject><subject>Hydroxyl ions</subject><subject>I.R. radiation</subject><subject>Imaging</subject><subject>In vivo methods and tests</subject><subject>Infrared Rays</subject><subject>Ion concentration</subject><subject>Ions</subject><subject>Micelles</subject><subject>Molecular Structure</subject><subject>Nanoparticles</subject><subject>Near infrared</subject><subject>Near infrared radiation</subject><subject>Optical Imaging</subject><subject>organic dye</subject><subject>Particle Size</subject><subject>pH effects</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>polymer micelle</subject><subject>Polymers</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Polystyrenes - chemistry</subject><subject>Sectioning</subject><subject>Surface Properties</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUuP0zAUhS0EYsrAmh2yxIZNZpzrOI8l6nRmKhWmErC2HOe6kyqxi52C8iP4z-PQUCQkNn7d7xz7-hDyNmVXkKb5tbKqC7q9AvZluWXVM7JIeVYmAFn-nCxYlbIk5xm7IK9C2DOWQgnwklxwzjOooFiQXytjUA-BOkO33mkMobU7erinztJV38ZtXKztgDa0wzhhDz_QJyljjNqefkblk7U1Xnls6G13dB6DRqtxQm9GTDZONbG0dd3Yo6efWo1dh_RnOzz-PgzD6NEiXUbla_LCxIbwzTxfkm-3q6_L-2TzcLdeftwkWohqSEowKJiCrG7qvCiKRom0KjlUyLABpYRBaGrNCs6NEVrUXJkMGuDCZFWBDb8kH06-B---HzEMMnY6vUtZdMcgIStSwav4cxF9_w-6d0c_fbsEwUrGAHIeqesTpb0LwaORB9_2yo8yZXJKSs5JyTmpqHg3-x7rHpsz_yeaCLATEGLJ7tD_vfj_nquTZB8GtcOzp_JDqzs887yQfBpm3bmuH5WXaPkTWOG6yA</recordid><startdate>20210310</startdate><enddate>20210310</enddate><creator>UMEZAWA, Masakazu</creator><creator>HARUKI, Mae</creator><creator>YOSHIDA, Moe</creator><creator>KAMIMURA, Masao</creator><creator>SOGA, Kohei</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210310</creationdate><title>Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core</title><author>UMEZAWA, Masakazu ; HARUKI, Mae ; YOSHIDA, Moe ; KAMIMURA, Masao ; SOGA, Kohei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c559t-82fe50a24bdb6777da5198329e0ed2aa5fe2dbc0733ff5c5b3af42d235f497ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Angiography</topic><topic>Aqueous solutions</topic><topic>Biocompatibility</topic><topic>Biological analysis</topic><topic>Chemistry</topic><topic>Dyes</topic><topic>Emission</topic><topic>Emissions</topic><topic>Fluorescence</topic><topic>fluorescence bioimaging</topic><topic>Fluorescent dyes</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Hydrogen ion concentration</topic><topic>Hydrogen ions</topic><topic>Hydrophobicity</topic><topic>Hydroxyl ions</topic><topic>I.R. radiation</topic><topic>Imaging</topic><topic>In vivo methods and tests</topic><topic>Infrared Rays</topic><topic>Ion concentration</topic><topic>Ions</topic><topic>Micelles</topic><topic>Molecular Structure</topic><topic>Nanoparticles</topic><topic>Near infrared</topic><topic>Near infrared radiation</topic><topic>Optical Imaging</topic><topic>organic dye</topic><topic>Particle Size</topic><topic>pH effects</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>polymer micelle</topic><topic>Polymers</topic><topic>Polystyrene</topic><topic>Polystyrene resins</topic><topic>Polystyrenes - chemistry</topic><topic>Sectioning</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UMEZAWA, Masakazu</creatorcontrib><creatorcontrib>HARUKI, Mae</creatorcontrib><creatorcontrib>YOSHIDA, Moe</creatorcontrib><creatorcontrib>KAMIMURA, Masao</creatorcontrib><creatorcontrib>SOGA, Kohei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UMEZAWA, Masakazu</au><au>HARUKI, Mae</au><au>YOSHIDA, Moe</au><au>KAMIMURA, Masao</au><au>SOGA, Kohei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. SCI</stitle><addtitle>Anal Sci</addtitle><date>2021-03-10</date><risdate>2021</risdate><volume>37</volume><issue>3</issue><spage>485</spage><epage>490</epage><pages>485-490</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>Fluorescence imaging using the over-thousand-nanometer (OTN) near-infrared (NIR) light is an emerging method for an in vivo imaging analysis of deep tissues without physical sectioning. Polymer micelle nanoparticles (PNPs) composed of organic polymers encapsulating an OTN-NIR fluorescent dye, IR-1061, in their hydrophobic core are expected to be biocompatible probes. Because IR-1061 quickly quenches due to the vibration of polar hydroxyl bonding in its surroundings, the influence of hydroxyl ions should be minimized. Herein, we investigated the effect of the hydrogen ion concentration during the preparation process using IR-1061 and an organic polymer, poly(ethylene glycol)-block-polystyrene (PEG-b-PSt), on the emission properties of the obtained OTN-PNPs. The OTN-PNP has a hydrodynamic diameter of 20 – 30 nm and emits 1110-nm fluorescence that is applicable to angiography. The loading efficiency of IR-1061 in the OTN-PNPs increased when prepared in an aqueous solution with a low hydroxyl ion concentration. In this solution (pH 3.0), highly emissive OTN-PNPs was obtained with IR-1061 at lower nominal concentrations. Decreasing the hydroxyl ion concentration during the preparation process yields highly emissive OTN-PNPs, which may improve the in vivo imaging analysis of biological phenomena in deep tissues.</abstract><cop>Singapore</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>33342927</pmid><doi>10.2116/analsci.20SCP09</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0910-6340 |
| ispartof | Analytical Sciences, 2021/03/10, Vol.37(3), pp.485-490 |
| issn | 0910-6340 1348-2246 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2471539340 |
| source | J-STAGE Free; MEDLINE; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | Analytical Chemistry Angiography Aqueous solutions Biocompatibility Biological analysis Chemistry Dyes Emission Emissions Fluorescence fluorescence bioimaging Fluorescent dyes Fluorescent Dyes - chemistry Fluorescent indicators Hydrogen ion concentration Hydrogen ions Hydrophobicity Hydroxyl ions I.R. radiation Imaging In vivo methods and tests Infrared Rays Ion concentration Ions Micelles Molecular Structure Nanoparticles Near infrared Near infrared radiation Optical Imaging organic dye Particle Size pH effects Polyethylene glycol Polyethylene Glycols - chemistry polymer micelle Polymers Polystyrene Polystyrene resins Polystyrenes - chemistry Sectioning Surface Properties |
| title | Effects of Processing pH on Emission Intensity of Over-1000 nm Near-Infrared Fluorescence of Dye-Loaded Polymer Micelle with Polystyrene Core |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A53%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Processing%20pH%20on%20Emission%20Intensity%20of%20Over-1000%20nm%20Near-Infrared%20Fluorescence%20of%20Dye-Loaded%20Polymer%20Micelle%20with%20Polystyrene%20Core&rft.jtitle=Analytical%20Sciences&rft.au=UMEZAWA,%20Masakazu&rft.date=2021-03-10&rft.volume=37&rft.issue=3&rft.spage=485&rft.epage=490&rft.pages=485-490&rft.issn=0910-6340&rft.eissn=1348-2246&rft_id=info:doi/10.2116/analsci.20SCP09&rft_dat=%3Cproquest_cross%3E2471539340%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2508002263&rft_id=info:pmid/33342927&rfr_iscdi=true |