Rhythmic adenosine triphosphate release from the cyanobacterial circadian clock protein KaiC revealed by real‐time monitoring of bioluminescence using firefly luciferase

The cyanobacterial circadian clock is composed of three clock proteins, KaiA, KaiB and KaiC. This KaiABC clock system can be reconstituted in vitro in the presence of adenosine triphosphate (ATP) and Mg2+, and shows circadian rhythms in the phosphorylation level and ATPase activity of KaiC. Previous...

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Veröffentlicht in:	Genes to cells : devoted to molecular & cellular mechanisms 2021-02, Vol.26 (2), p.83-93
Hauptverfasser:	Mutoh, Risa, Iwata, Keita, Iida, Takahiro, Ishiura, Masahiro, Onai, Kiyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine triphosphatase Adenosine triphosphate ATP release Bioluminescence circadian clock Circadian rhythm Circadian rhythms CLOCK protein cyanobacteria firefly luciferase assay KaiC Magnesium Phosphorylation real‐time monitoring
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