Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy

Aim Ectopic pregnancy is a life‐threatening problem in reproductive ages. Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pre...

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Veröffentlicht in:The journal of obstetrics and gynaecology research 2021-03, Vol.47 (3), p.921-927
Hauptverfasser: Sahin, Cagdas, Uygun, Zihni Onur, Hortu, Ismet, Akdemir, Ali, Kocamanoglu, Meltem, Ergenoglu, Ahmet M., Akcay, Yasemin
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container_issue 3
container_start_page 921
container_title The journal of obstetrics and gynaecology research
container_volume 47
creator Sahin, Cagdas
Uygun, Zihni Onur
Hortu, Ismet
Akdemir, Ali
Kocamanoglu, Meltem
Ergenoglu, Ahmet M.
Akcay, Yasemin
description Aim Ectopic pregnancy is a life‐threatening problem in reproductive ages. Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pregnancy with new promising markers provided different aspects for diagnosing ectopic pregnancy in the present study. Methods In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n = 46), intrauterine pregnant patients as intrauterine pregnancy group (n = 29) and not‐pregnant patients as nonpregnancy group (n = 10) participated to study. Cervical fluid samples were collected with using merocel sponge. In addition, serum samples were obtained from patients. Dynein heavy chain 5 (DNAH5) and creatine kinase (CK) levels were determined by enzyme‐linked immunosorbent assay kits in samples. Results Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25–75% percentile 0–9.56 ng/mL vs median 6.14 ng/mL; 1.40–8.31 ng/mL; P 
doi_str_mv 10.1111/jog.14616
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Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pregnancy with new promising markers provided different aspects for diagnosing ectopic pregnancy in the present study. Methods In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n = 46), intrauterine pregnant patients as intrauterine pregnancy group (n = 29) and not‐pregnant patients as nonpregnancy group (n = 10) participated to study. Cervical fluid samples were collected with using merocel sponge. In addition, serum samples were obtained from patients. Dynein heavy chain 5 (DNAH5) and creatine kinase (CK) levels were determined by enzyme‐linked immunosorbent assay kits in samples. Results Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25–75% percentile 0–9.56 ng/mL vs median 6.14 ng/mL; 1.40–8.31 ng/mL; P &lt; 0.001). On the other hand, DNAH5 protein was not detected in nonpregnant patients' samples. In addition, statistical significant increased cervical fluid CK levels were diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 4477.61 IU/L; 0–64 925.37 IU/L vs 0 IU/L; 0–6832.30 IU/L; P = 0.006). Conclusion Measuring of CK and DNAH5 in cervical fluid could be promising markers for early diagnosing of ectopic pregnancy. Decreased DNAH5 levels in cervical fluid might be result from abnormal cilia function in ectopic pregnant patients. ClinicalTrials.gov ID. NCT02995356.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.14616</identifier><identifier>PMID: 33336538</identifier><language>eng</language><publisher>Kyoto, Japan: John Wiley &amp; Sons Australia, Ltd</publisher><subject>cervical fluid ; Cervix ; Cilia ; Creatine ; Creatine kinase ; Dynein ; dynein heavy chain5 ; Ectopic pregnancy ; Medical treatment ; Morbidity ; Patients ; Pregnancy ; Pregnancy complications ; Statistical analysis</subject><ispartof>The journal of obstetrics and gynaecology research, 2021-03, Vol.47 (3), p.921-927</ispartof><rights>2020 Japan Society of Obstetrics and Gynecology</rights><rights>2020 Japan Society of Obstetrics and Gynecology.</rights><rights>2021 Japan Society of Obstetrics and Gynecology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-461314fc7ac0109c5137bf7b441e883e722e8b9351fe0daafab2d37e35c893f53</citedby><cites>FETCH-LOGICAL-c3536-461314fc7ac0109c5137bf7b441e883e722e8b9351fe0daafab2d37e35c893f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.14616$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.14616$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33336538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahin, Cagdas</creatorcontrib><creatorcontrib>Uygun, Zihni Onur</creatorcontrib><creatorcontrib>Hortu, Ismet</creatorcontrib><creatorcontrib>Akdemir, Ali</creatorcontrib><creatorcontrib>Kocamanoglu, Meltem</creatorcontrib><creatorcontrib>Ergenoglu, Ahmet M.</creatorcontrib><creatorcontrib>Akcay, Yasemin</creatorcontrib><title>Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J Obstet Gynaecol Res</addtitle><description>Aim Ectopic pregnancy is a life‐threatening problem in reproductive ages. Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pregnancy with new promising markers provided different aspects for diagnosing ectopic pregnancy in the present study. Methods In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n = 46), intrauterine pregnant patients as intrauterine pregnancy group (n = 29) and not‐pregnant patients as nonpregnancy group (n = 10) participated to study. Cervical fluid samples were collected with using merocel sponge. In addition, serum samples were obtained from patients. Dynein heavy chain 5 (DNAH5) and creatine kinase (CK) levels were determined by enzyme‐linked immunosorbent assay kits in samples. Results Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25–75% percentile 0–9.56 ng/mL vs median 6.14 ng/mL; 1.40–8.31 ng/mL; P &lt; 0.001). On the other hand, DNAH5 protein was not detected in nonpregnant patients' samples. In addition, statistical significant increased cervical fluid CK levels were diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 4477.61 IU/L; 0–64 925.37 IU/L vs 0 IU/L; 0–6832.30 IU/L; P = 0.006). Conclusion Measuring of CK and DNAH5 in cervical fluid could be promising markers for early diagnosing of ectopic pregnancy. Decreased DNAH5 levels in cervical fluid might be result from abnormal cilia function in ectopic pregnant patients. ClinicalTrials.gov ID. NCT02995356.</description><subject>cervical fluid</subject><subject>Cervix</subject><subject>Cilia</subject><subject>Creatine</subject><subject>Creatine kinase</subject><subject>Dynein</subject><subject>dynein heavy chain5</subject><subject>Ectopic pregnancy</subject><subject>Medical treatment</subject><subject>Morbidity</subject><subject>Patients</subject><subject>Pregnancy</subject><subject>Pregnancy complications</subject><subject>Statistical analysis</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kUFPJCEQhYlxo6568A8YEi96aAcGaLqPZqLubkzmsp47NF20jAyMMD2m458XZ9TDJlsXKqmP9woeQmeUXNNck0XorykvabmHjijnsiBSlPu5Z5wWFZHlIfqZ0oIQKmtaHaBDlqsUrDpCb4_J-h53owfr8ROozYj1k8q9wMp3WEdQa-sBP1uvEmAHG3AJ57mGuLFaOWzcYLst3LoQOmxCxKCiG3FnVe_D1iAYDHodVlbjVYTeK6_HE_TDKJfg9PM8Ro93t39nv4qH-f3v2c1DoZlgZZEfxig3WipNKKm1oEy2RracU6gqBnI6haqtmaAGSKeUUe20YxKY0FXNjGDH6HKnu4rhZYC0bpY2aXBOeQhDaqZc5t-TgvCMXvyDLsIQfd4uU3V2rkXFMnW1o3QMKUUwzSrapYpjQ0nzkUi-1TfbRDJ7_qk4tEvovsmvCDIw2QGv1sH4f6Xmz_x-J_kOnIeVEA</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Sahin, Cagdas</creator><creator>Uygun, Zihni Onur</creator><creator>Hortu, Ismet</creator><creator>Akdemir, Ali</creator><creator>Kocamanoglu, Meltem</creator><creator>Ergenoglu, Ahmet M.</creator><creator>Akcay, Yasemin</creator><general>John Wiley &amp; Sons Australia, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>202103</creationdate><title>Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy</title><author>Sahin, Cagdas ; Uygun, Zihni Onur ; Hortu, Ismet ; Akdemir, Ali ; Kocamanoglu, Meltem ; Ergenoglu, Ahmet M. ; Akcay, Yasemin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-461314fc7ac0109c5137bf7b441e883e722e8b9351fe0daafab2d37e35c893f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>cervical fluid</topic><topic>Cervix</topic><topic>Cilia</topic><topic>Creatine</topic><topic>Creatine kinase</topic><topic>Dynein</topic><topic>dynein heavy chain5</topic><topic>Ectopic pregnancy</topic><topic>Medical treatment</topic><topic>Morbidity</topic><topic>Patients</topic><topic>Pregnancy</topic><topic>Pregnancy complications</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahin, Cagdas</creatorcontrib><creatorcontrib>Uygun, Zihni Onur</creatorcontrib><creatorcontrib>Hortu, Ismet</creatorcontrib><creatorcontrib>Akdemir, Ali</creatorcontrib><creatorcontrib>Kocamanoglu, Meltem</creatorcontrib><creatorcontrib>Ergenoglu, Ahmet M.</creatorcontrib><creatorcontrib>Akcay, Yasemin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahin, Cagdas</au><au>Uygun, Zihni Onur</au><au>Hortu, Ismet</au><au>Akdemir, Ali</au><au>Kocamanoglu, Meltem</au><au>Ergenoglu, Ahmet M.</au><au>Akcay, Yasemin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J Obstet Gynaecol Res</addtitle><date>2021-03</date><risdate>2021</risdate><volume>47</volume><issue>3</issue><spage>921</spage><epage>927</epage><pages>921-927</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aim Ectopic pregnancy is a life‐threatening problem in reproductive ages. Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pregnancy with new promising markers provided different aspects for diagnosing ectopic pregnancy in the present study. Methods In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n = 46), intrauterine pregnant patients as intrauterine pregnancy group (n = 29) and not‐pregnant patients as nonpregnancy group (n = 10) participated to study. Cervical fluid samples were collected with using merocel sponge. In addition, serum samples were obtained from patients. Dynein heavy chain 5 (DNAH5) and creatine kinase (CK) levels were determined by enzyme‐linked immunosorbent assay kits in samples. Results Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25–75% percentile 0–9.56 ng/mL vs median 6.14 ng/mL; 1.40–8.31 ng/mL; P &lt; 0.001). On the other hand, DNAH5 protein was not detected in nonpregnant patients' samples. In addition, statistical significant increased cervical fluid CK levels were diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 4477.61 IU/L; 0–64 925.37 IU/L vs 0 IU/L; 0–6832.30 IU/L; P = 0.006). Conclusion Measuring of CK and DNAH5 in cervical fluid could be promising markers for early diagnosing of ectopic pregnancy. Decreased DNAH5 levels in cervical fluid might be result from abnormal cilia function in ectopic pregnant patients. ClinicalTrials.gov ID. NCT02995356.</abstract><cop>Kyoto, Japan</cop><pub>John Wiley &amp; Sons Australia, Ltd</pub><pmid>33336538</pmid><doi>10.1111/jog.14616</doi><tpages>7</tpages></addata></record>
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subjects cervical fluid
Cervix
Cilia
Creatine
Creatine kinase
Dynein
dynein heavy chain5
Ectopic pregnancy
Medical treatment
Morbidity
Patients
Pregnancy
Pregnancy complications
Statistical analysis
title Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy
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