Biochemical characterization and molecular docking of cloned xylanase gene from Bacillus subtilis RTS expressed in E. coli

This study employed mesophilic Bacillus subtilis RTS strain isolated from soil with high xylanolytic activity. A 642 bp (xyn) xylanase gene (GenBank accession number MT677937) was extracted from Bacillus subtilis RTS and cloned in Escherichia coli BL21 cells using pET21c expression system. The clone...

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Veröffentlicht in:	International journal of biological macromolecules 2021-01, Vol.168, p.310-321
Hauptverfasser:	Saleem, Aimen, Waris, Saboora, Ahmed, Toheed, Tabassum, Romana
Format:	Artikel
Sprache:	eng
Schlagworte:	AutoDock Vina Bacillus - enzymology Bacillus subtilis - enzymology Bacillus subtilis - genetics Cloning Cloning, Molecular - methods Endo-1,4-beta Xylanases - chemistry Endo-1,4-beta Xylanases - genetics Endo-1,4-beta Xylanases - isolation & purification Enzyme Stability Escherichia coli - genetics Genetic characterization Hydrogen-Ion Concentration Molecular Docking Simulation Recombinant Proteins - chemistry Sequencing Temperature
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