Click-ExM enables expansion microscopy for all biomolecules

Expansion microscopy (ExM) allows super-resolution imaging on conventional fluorescence microscopes, but has been limited to proteins and nucleic acids. Here we develop click-ExM, which integrates click labeling into ExM to enable a ‘one-stop-shop’ method for nanoscale imaging of various types of bi...

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Veröffentlicht in:	Nature methods 2021-01, Vol.18 (1), p.107-113
Hauptverfasser:	Sun, De-en, Fan, Xinqi, Shi, Yujie, Zhang, Hao, Huang, Zhimin, Cheng, Bo, Tang, Qi, Li, Wei, Zhu, Yuntao, Bai, Jinyi, Liu, Wei, Li, Yuan, Wang, Xiaoting, Lei, Xiaoguang, Chen, Xing
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/328/2238 631/92/96 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomolecules Biotin Brain - metabolism Cell culture Click Chemistry Deoxyribonucleic acid DNA DNA - analysis Fluorescence Fluorescence microscopy HeLa Cells Humans Image resolution Imaging, Three-Dimensional - methods Labeling Labels Life Sciences Lipids Lipids - analysis Macromolecular Substances - analysis Methods Mice Mice, Inbred C57BL Microscopes Microscopy Microscopy, Fluorescence - methods Myocytes, Cardiac - metabolism Nucleic acids Observations Organ Specificity Polysaccharides Polysaccharides - analysis Proteins Proteins - analysis Proteomics Rats Rats, Sprague-Dawley Ribonucleic acid RNA RNA - analysis Streptavidin Tissue culture
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