Live cell PNA labelling enables erasable fluorescence imaging of membrane proteins

DNA nanotechnology is an emerging field that promises fascinating opportunities for the manipulation and imaging of proteins on a cell surface. The key to progress is the ability to create a nucleic acid–protein junction in the context of living cells. Here we report a covalent labelling reaction th...

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Veröffentlicht in:	Nature chemistry 2021-01, Vol.13 (1), p.15-23
Hauptverfasser:	Gavins, Georgina C., Gröger, Katharina, Bartoschek, Michael D., Wolf, Philipp, Beck-Sickinger, Annette G., Bultmann, Sebastian, Seitz, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	639/638/11/874 639/638/92/610 639/638/92/611 639/638/92/612/1237 639/638/92/96 Acids Amino Acid Sequence Analytical Chemistry Animals Biochemistry Cell surface Chemical compounds Chemistry Chemistry and Materials Science Chemistry/Food Science CHO Cells Coils Color removal Cricetinae Cricetulus Dyes Endothelins Epidermal growth factor ErbB Receptors - chemistry ErbB Receptors - genetics ErbB Receptors - metabolism Fluorescence Fluorescent dyes Fluorescent indicators Fluorophores Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Growth factors HEK293 Cells Humans Hybridization Imaging Inorganic Chemistry Internalization Labeling Landing Membrane proteins Microscopy, Fluorescence Monitoring methods Nanotechnology Nucleic Acid Hybridization Nucleic acids Organic Chemistry Peptide nucleic acids Peptide Nucleic Acids - chemical synthesis Peptide Nucleic Acids - chemistry Peptide Nucleic Acids - metabolism Peptides Peptides - chemical synthesis Peptides - chemistry Peptides - metabolism Physical Chemistry Protein Binding Proteins Receptor, Endothelin B - chemistry Receptor, Endothelin B - genetics Receptor, Endothelin B - metabolism Receptors Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Recruitment
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