Rapid Purification of Immunoglobulin G Using a Protein A-​immobilized Monolithic Spin Column with Hydrophilic Polymers

Rapid Purification of Immunoglobulin G Using a Protein A-​immobilized Monolithic Spin Column with Hydrophilic Polymers

A rapid purification method was developed for antibody production in Chinese hamster ovary (CHO) cells using a Protein A-immobilized monolithic silica spin column with hydrophilic polymers. Monolithic silica modified with copolymers of 2-hydroxyethylmethacrylate (HEMA) and glycidyl methacrylate (GMA...

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Veröffentlicht in:Analytical Sciences 2021/07/10, Vol.37(7), pp.985-990
Hauptverfasser: OTA, Shigenori, YUI, Yuko, SATO, Tsutomu, YOSHIMOTO, Noriko, YAMAMOTO, Shuichi
Format: Artikel
Sprache:eng
Schlagworte:
affinity chromatography
Analytical Chemistry
Animals
Antibodies
Chemistry
CHO Cells
Copolymers
Correlation coefficient
Correlation coefficients
Cricetinae
Cricetulus
Hydrophilicity
Hydrophobic and Hydrophilic Interactions
IgG antibody
Immunoglobulin G
Monolithic silica
non-specific adsorption
Original Paper
Polyhydroxyethyl methacrylate
Polymers
Protein A
Protein purification
Proteins
Purification
Reagents
Silica
Silicon dioxide
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