Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine
The present study was designed to investigate the effects of different levels of l-carnitine (LC) on sperm quality factor (SQF), alterations in testis fatty acid profiles, testicular histology and reproductive hormones in young roosters. Eighteen broiler breeders (Ross 308) weighed at 3 months of ag...
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| creator | Mohammadi, Vahid Sharifi, Seyed Davood Sharafi, Mohsen Mohammadi- Sangcheshmeh, Abdollah Shahverdi, Abdolhossein Alizadeh, AliReza |
| description | The present study was designed to investigate the effects of different levels of l-carnitine (LC) on sperm quality factor (SQF), alterations in testis fatty acid profiles, testicular histology and reproductive hormones in young roosters. Eighteen broiler breeders (Ross 308) weighed at 3 months of age. They were randomly classified while each group had six birds. There were three experimental groups based on the LC concentrations (i.e. LC-0, LC-250, LC-500 mg per kg of diet). After two weeks of adaptation, semen samples were collected and evaluated for seminal attributes every two weeks (from week 24 to week 34). At the end of the experiments, four roosters from each treatment group were sacrificed in order to analyze testicular histology, testis fatty acid profiles and reproductive hormones. Supplementing the diet with two of the LC levels for 22 weeks caused significant rise in sperm concentration, viability and SQF compared to that of the control group (P |
| doi_str_mv | 10.1016/j.theriogenology.2020.10.005 |
| format | Article |
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•Sperm quality factor (SQF) were higher by l-carnitine compared to control.•The l-carnitine supplementation improved rooster's testosterone levels.•Dietary l-carnitine increased the percentage of omega-3 fatty acids in testis.•These improvements declare the manipulation of fatty acid profiles by l-carnitine.]]></description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2020.10.005</identifier><identifier>PMID: 33278693</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animal Feed - analysis ; Animals ; Carnitine ; Chickens ; Dietary Supplements ; Fatty acid profiles ; Fatty Acids ; l-carnitine ; Male ; Rooster ; Semen Analysis - veterinary ; Semen quality ; Spermatozoa ; Testicular histology ; Testis ; Testosterone</subject><ispartof>Theriogenology, 2021-02, Vol.161, p.8-15</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-2bc06b01fa68acba283fe280fca665f7f215fec5f416f358f5c8e3032212fdc43</citedby><cites>FETCH-LOGICAL-c386t-2bc06b01fa68acba283fe280fca665f7f215fec5f416f358f5c8e3032212fdc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X20305379$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33278693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohammadi, Vahid</creatorcontrib><creatorcontrib>Sharifi, Seyed Davood</creatorcontrib><creatorcontrib>Sharafi, Mohsen</creatorcontrib><creatorcontrib>Mohammadi- Sangcheshmeh, Abdollah</creatorcontrib><creatorcontrib>Shahverdi, Abdolhossein</creatorcontrib><creatorcontrib>Alizadeh, AliReza</creatorcontrib><title>Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description><![CDATA[The present study was designed to investigate the effects of different levels of l-carnitine (LC) on sperm quality factor (SQF), alterations in testis fatty acid profiles, testicular histology and reproductive hormones in young roosters. Eighteen broiler breeders (Ross 308) weighed at 3 months of age. They were randomly classified while each group had six birds. There were three experimental groups based on the LC concentrations (i.e. LC-0, LC-250, LC-500 mg per kg of diet). After two weeks of adaptation, semen samples were collected and evaluated for seminal attributes every two weeks (from week 24 to week 34). At the end of the experiments, four roosters from each treatment group were sacrificed in order to analyze testicular histology, testis fatty acid profiles and reproductive hormones. Supplementing the diet with two of the LC levels for 22 weeks caused significant rise in sperm concentration, viability and SQF compared to that of the control group (P < 0.05). Quadratic analysis in terms of number of seminiferous tubules and spermatogenesis index were significant (P<0.05). Tubular differentiation index improved linearly by the increasing levels of LC supplementation (P<0.01). The analysis of fatty acid profiles showed that LC significantly (P < 0.05) reduced the percentages of C14:0, C21:0, total saturated fatty acids, total odd-chain fatty acids and n-6/n-3 ratio. Moreover, LC significantly increased the percentage of C20:5n-3 (Eicosapentaenoic acid; EPA) (P < 0.05). Analysis of the correlation coefficient revealed that the SQF is in consistency with EPA (r = 0.98; P < 0.04). In contrast, SQF negatively and significantly correlates with odd-chain fatty acids (r = - 0.99; P < 0.001). The desaturation index for C16 fatty acids (16:1cis/C16:0) negligibly increased linearly as LC was added to the diet (P < 0.05). Furthermore, LC caused the roosters to have significant (P < 0.05) high levels of total testosterone and FSH concentrations. The concentration of LH in different treatment groups, however, turned out to be similar in response to the different levels of LC. In conclusion, long-term supplementation of rooster diet with LC can have beneficial effects on SQF and testis histology. The benefits include alterations in testicular histology, reproductive hormones and testicular fatty acid profiles.
•Sperm quality factor (SQF) were higher by l-carnitine compared to control.•The l-carnitine supplementation improved rooster's testosterone levels.•Dietary l-carnitine increased the percentage of omega-3 fatty acids in testis.•These improvements declare the manipulation of fatty acid profiles by l-carnitine.]]></description><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Carnitine</subject><subject>Chickens</subject><subject>Dietary Supplements</subject><subject>Fatty acid profiles</subject><subject>Fatty Acids</subject><subject>l-carnitine</subject><subject>Male</subject><subject>Rooster</subject><subject>Semen Analysis - veterinary</subject><subject>Semen quality</subject><subject>Spermatozoa</subject><subject>Testicular histology</subject><subject>Testis</subject><subject>Testosterone</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS1ERYfCKyAvWLAgU_9MPBmJDaooILXqBiR2luNcd-4osae2M-rseA3ehmfhSXCagsSOlS2f75zrq0PIa86WnHF1vlvmLUQMt-BDH26PS8HEJC0Zq5-QBW_Wm0oKyZ-SBWMbWakN_3ZKnqe0Y4xJpfgzciqlWDdqIxfk57XxuB97kzF4Ghx1JucjNRY7uo_BYQ-JoqcxhJQhpl_ff9AM5ZreUtOXl9lYiAT3o-npNsQh-EnGoQQcYACfHyImG9oyKtItpvzweWq3JhpbcnASEzW-o9DDYY5Ne4gDvSu5WD7lChgibY_0qrImeszo4QU5caZP8PLxPCNfLz98ufhUXd18_HzxvpCyUbkSrWWqZdwZ1RjbGtFIB6Jhzhqlard2gtcObO1WXDlZN662DUgmheDCdXYlz8ibObcsdTeWVfSAyULfGw9hTFqs1FpxWStW0HczamNIKYLT-4iDiUfNmZ461Dv9b4d66nBSS4fF_upx0tgO0P01_ymtAJczAGXfA0LUySJ4Cx1GsFl3Af9v0m-_c77Q</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Mohammadi, Vahid</creator><creator>Sharifi, Seyed Davood</creator><creator>Sharafi, Mohsen</creator><creator>Mohammadi- Sangcheshmeh, Abdollah</creator><creator>Shahverdi, Abdolhossein</creator><creator>Alizadeh, AliReza</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202102</creationdate><title>Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine</title><author>Mohammadi, Vahid ; Sharifi, Seyed Davood ; Sharafi, Mohsen ; Mohammadi- Sangcheshmeh, Abdollah ; Shahverdi, Abdolhossein ; Alizadeh, AliReza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-2bc06b01fa68acba283fe280fca665f7f215fec5f416f358f5c8e3032212fdc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Feed - analysis</topic><topic>Animals</topic><topic>Carnitine</topic><topic>Chickens</topic><topic>Dietary Supplements</topic><topic>Fatty acid profiles</topic><topic>Fatty Acids</topic><topic>l-carnitine</topic><topic>Male</topic><topic>Rooster</topic><topic>Semen Analysis - veterinary</topic><topic>Semen quality</topic><topic>Spermatozoa</topic><topic>Testicular histology</topic><topic>Testis</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammadi, Vahid</creatorcontrib><creatorcontrib>Sharifi, Seyed Davood</creatorcontrib><creatorcontrib>Sharafi, Mohsen</creatorcontrib><creatorcontrib>Mohammadi- Sangcheshmeh, Abdollah</creatorcontrib><creatorcontrib>Shahverdi, Abdolhossein</creatorcontrib><creatorcontrib>Alizadeh, AliReza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammadi, Vahid</au><au>Sharifi, Seyed Davood</au><au>Sharafi, Mohsen</au><au>Mohammadi- Sangcheshmeh, Abdollah</au><au>Shahverdi, Abdolhossein</au><au>Alizadeh, AliReza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2021-02</date><risdate>2021</risdate><volume>161</volume><spage>8</spage><epage>15</epage><pages>8-15</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract><![CDATA[The present study was designed to investigate the effects of different levels of l-carnitine (LC) on sperm quality factor (SQF), alterations in testis fatty acid profiles, testicular histology and reproductive hormones in young roosters. Eighteen broiler breeders (Ross 308) weighed at 3 months of age. They were randomly classified while each group had six birds. There were three experimental groups based on the LC concentrations (i.e. LC-0, LC-250, LC-500 mg per kg of diet). After two weeks of adaptation, semen samples were collected and evaluated for seminal attributes every two weeks (from week 24 to week 34). At the end of the experiments, four roosters from each treatment group were sacrificed in order to analyze testicular histology, testis fatty acid profiles and reproductive hormones. Supplementing the diet with two of the LC levels for 22 weeks caused significant rise in sperm concentration, viability and SQF compared to that of the control group (P < 0.05). Quadratic analysis in terms of number of seminiferous tubules and spermatogenesis index were significant (P<0.05). Tubular differentiation index improved linearly by the increasing levels of LC supplementation (P<0.01). The analysis of fatty acid profiles showed that LC significantly (P < 0.05) reduced the percentages of C14:0, C21:0, total saturated fatty acids, total odd-chain fatty acids and n-6/n-3 ratio. Moreover, LC significantly increased the percentage of C20:5n-3 (Eicosapentaenoic acid; EPA) (P < 0.05). Analysis of the correlation coefficient revealed that the SQF is in consistency with EPA (r = 0.98; P < 0.04). In contrast, SQF negatively and significantly correlates with odd-chain fatty acids (r = - 0.99; P < 0.001). The desaturation index for C16 fatty acids (16:1cis/C16:0) negligibly increased linearly as LC was added to the diet (P < 0.05). Furthermore, LC caused the roosters to have significant (P < 0.05) high levels of total testosterone and FSH concentrations. The concentration of LH in different treatment groups, however, turned out to be similar in response to the different levels of LC. In conclusion, long-term supplementation of rooster diet with LC can have beneficial effects on SQF and testis histology. The benefits include alterations in testicular histology, reproductive hormones and testicular fatty acid profiles.
•Sperm quality factor (SQF) were higher by l-carnitine compared to control.•The l-carnitine supplementation improved rooster's testosterone levels.•Dietary l-carnitine increased the percentage of omega-3 fatty acids in testis.•These improvements declare the manipulation of fatty acid profiles by l-carnitine.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33278693</pmid><doi>10.1016/j.theriogenology.2020.10.005</doi><tpages>8</tpages></addata></record> |
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| subjects | Animal Feed - analysis Animals Carnitine Chickens Dietary Supplements Fatty acid profiles Fatty Acids l-carnitine Male Rooster Semen Analysis - veterinary Semen quality Spermatozoa Testicular histology Testis Testosterone |
| title | Manipulation of fatty acid profiles in roosters’ testes, alteration in sexual hormones, improvements in testicular histology characteristics and elevation sperm quality factor by L-carnitine |
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