Nerve guidance conduit promoted peripheral nerve regeneration in rats
Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half‐life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we...
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| Veröffentlicht in: | Artificial organs 2021-06, Vol.45 (6), p.616-624 |
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| creator | Long, Qingshan Wu, Bingshan Yang, Yu Wang, Shanhong Shen, Yiwen Bao, Qinghua Xu, Feng |
| description | Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half‐life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we cross‐linked heparin to collagen tubes to construct nerve guidance conduits for delivering NGF. The conduits were implanted to bridge a facial nerve defect in rats. Histological and functional analyses were performed to assess the effect of the nerve guidance conduit on facial nerve regeneration. Heparin enhanced the binding of NGF to collagen while retaining its bioactivity. Also, the nerve guidance conduit significantly promoted axonal growth and Schwan cell proliferation at 12 weeks after surgery. The nerve regeneration and functional recovery outcomes using the nerve guidance conduit were similar to those of autologous nerve grafting. Therefore, the nerve guidance conduit may promote safer nerve regeneration.
NGF was immobilized in collagen tube to promote nerve regeneration after facial nerve injury. |
| doi_str_mv | 10.1111/aor.13881 |
| format | Article |
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NGF was immobilized in collagen tube to promote nerve regeneration after facial nerve injury.</description><subject>Autografts</subject><subject>Axon guidance</subject><subject>Binding</subject><subject>Biocompatibility</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Biological activity</subject><subject>Body fluids</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Facial nerve</subject><subject>Growth factors</subject><subject>Heparin</subject><subject>Immunogenicity</subject><subject>Nerve growth factor</subject><subject>nerve guidance conduit</subject><subject>Nerve guides</subject><subject>nerve regeneration</subject><subject>peripheral nerve injury</subject><subject>Peripheral nerves</subject><subject>Recovery of function</subject><subject>Regeneration</subject><subject>Silicones</subject><subject>Surgery</subject><subject>Tubes</subject><issn>0160-564X</issn><issn>1525-1594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10E1LwzAYB_AgipvTg19ACl70UJf3Jscx5gsMB6LgLaTNs9nRtTNplX174zY9COaShPz48-SP0DnBNySuoW38DWFKkQPUJ4KKlAjND1EfE4lTIflrD52EsMQYZxzLY9RjjGaYStJHk0fwH5AsutLZuoCkaGrXlW2y9s2qacEla_Dl-g28rZJ6Sz0sIJ5sWzZ1UtZJPIVTdDS3VYCz_T5AL7eT5_F9Op3dPYxH07TgRJPUZq6wUgjmiOLSKj3Xec5EVkgOLtdKKaldfASs421OHWFU5VxLJiNTnA3Q1S43jvfeQWjNqgwFVJWtoemCoVzKLKNSsEgv_9Bl0_k6TmeoYPH3TFMc1fVOFb4JwcPcrH25sn5jCDbf3ZrYrdl2G-3FPrHLV-B-5U-ZEQx34LOsYPN_khnNnnaRX2Bjgb8</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Long, Qingshan</creator><creator>Wu, Bingshan</creator><creator>Yang, Yu</creator><creator>Wang, Shanhong</creator><creator>Shen, Yiwen</creator><creator>Bao, Qinghua</creator><creator>Xu, Feng</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7976-4446</orcidid></search><sort><creationdate>202106</creationdate><title>Nerve guidance conduit promoted peripheral nerve regeneration in rats</title><author>Long, Qingshan ; Wu, Bingshan ; Yang, Yu ; Wang, Shanhong ; Shen, Yiwen ; Bao, Qinghua ; Xu, Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4191-a7dca6553d1846a89f9bb357c64edb988869d3d1e09b98f2d1328b49636b35843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Autografts</topic><topic>Axon guidance</topic><topic>Binding</topic><topic>Biocompatibility</topic><topic>Biodegradability</topic><topic>Biodegradation</topic><topic>Biological activity</topic><topic>Body fluids</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Facial nerve</topic><topic>Growth factors</topic><topic>Heparin</topic><topic>Immunogenicity</topic><topic>Nerve growth factor</topic><topic>nerve guidance conduit</topic><topic>Nerve guides</topic><topic>nerve regeneration</topic><topic>peripheral nerve injury</topic><topic>Peripheral nerves</topic><topic>Recovery of function</topic><topic>Regeneration</topic><topic>Silicones</topic><topic>Surgery</topic><topic>Tubes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Long, Qingshan</creatorcontrib><creatorcontrib>Wu, Bingshan</creatorcontrib><creatorcontrib>Yang, Yu</creatorcontrib><creatorcontrib>Wang, Shanhong</creatorcontrib><creatorcontrib>Shen, Yiwen</creatorcontrib><creatorcontrib>Bao, Qinghua</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Long, Qingshan</au><au>Wu, Bingshan</au><au>Yang, Yu</au><au>Wang, Shanhong</au><au>Shen, Yiwen</au><au>Bao, Qinghua</au><au>Xu, Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerve guidance conduit promoted peripheral nerve regeneration in rats</atitle><jtitle>Artificial organs</jtitle><addtitle>Artif Organs</addtitle><date>2021-06</date><risdate>2021</risdate><volume>45</volume><issue>6</issue><spage>616</spage><epage>624</epage><pages>616-624</pages><issn>0160-564X</issn><eissn>1525-1594</eissn><abstract>Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half‐life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we cross‐linked heparin to collagen tubes to construct nerve guidance conduits for delivering NGF. The conduits were implanted to bridge a facial nerve defect in rats. Histological and functional analyses were performed to assess the effect of the nerve guidance conduit on facial nerve regeneration. Heparin enhanced the binding of NGF to collagen while retaining its bioactivity. Also, the nerve guidance conduit significantly promoted axonal growth and Schwan cell proliferation at 12 weeks after surgery. The nerve regeneration and functional recovery outcomes using the nerve guidance conduit were similar to those of autologous nerve grafting. Therefore, the nerve guidance conduit may promote safer nerve regeneration.
NGF was immobilized in collagen tube to promote nerve regeneration after facial nerve injury.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33270261</pmid><doi>10.1111/aor.13881</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7976-4446</orcidid></addata></record> |
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| ispartof | Artificial organs, 2021-06, Vol.45 (6), p.616-624 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Autografts Axon guidance Binding Biocompatibility Biodegradability Biodegradation Biological activity Body fluids Cell proliferation Collagen Facial nerve Growth factors Heparin Immunogenicity Nerve growth factor nerve guidance conduit Nerve guides nerve regeneration peripheral nerve injury Peripheral nerves Recovery of function Regeneration Silicones Surgery Tubes |
| title | Nerve guidance conduit promoted peripheral nerve regeneration in rats |
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