Expression of TOB/BTG family members in patients with inflammatory bowel disease

In recent years, the role of anti-proliferative TOB proteins in the regulation of immune response by inhibiting T cell activation has been demonstrated. Nevertheless, no previous studies have explored their expression in patients with IBD. The aim of the study was to characterize the gene and protei...

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Veröffentlicht in:Scandinavian journal of immunology 2021-04, Vol.93 (4), p.e13004-e13004
Hauptverfasser: Fonseca-Camarillo, Gabriela, Furuzawa-Carballeda, Janette, Priego-Ranero, Ángel A, Martínez-Benítez, Braulio, Barreto-Zúñiga, Rafael, Yamamoto-Furusho, Jesús K
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container_issue 4
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container_title Scandinavian journal of immunology
container_volume 93
creator Fonseca-Camarillo, Gabriela
Furuzawa-Carballeda, Janette
Priego-Ranero, Ángel A
Martínez-Benítez, Braulio
Barreto-Zúñiga, Rafael
Yamamoto-Furusho, Jesús K
description In recent years, the role of anti-proliferative TOB proteins in the regulation of immune response by inhibiting T cell activation has been demonstrated. Nevertheless, no previous studies have explored their expression in patients with IBD. The aim of the study was to characterize the gene and protein expression of the TOB/BTG family in intestinal tissue of patients with IBD. This is an observational and cross-sectional study that included 63 IBD patients. Gene expression of TOB/BTG family was measured by RT-PCR. Protein expression of TOB/CD16 and BTG/Ki-67 was evaluated by immunohistochemistry. TOB/BTG family mRNAs were detected and quantitated by RT-qPCR in rectal and ileum biopsies from UC patients and CD patients, respectively, and non-inflammatory control tissues. Results showed that TOB1 and BTG1 gene expression was decreased in the colonic mucosa from patients with UC compared with the control group. The TOB2 and BTG2 genes were over-expressed in the colonic mucosa of patients with UC in remission compared with the active UC and control group. The high TOB2 gene expression was associated with histological remission (P = .01). TOB1/CD16, TOB2/CD16, BTG1/Ki-67, BTG2/Ki-67 and BTG4/Ki-67 single and double positive cells were mostly NK, macrophages, epithelial cells, connective tissue cells and perivascular inflammatory infiltrates in tissues from patients with UC and CD. This is the first depiction of the TOB/BTG family gene and protein expression in rectal and ileum tissues by a CD16 subpopulation in IBD.
doi_str_mv 10.1111/sji.13004
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Nevertheless, no previous studies have explored their expression in patients with IBD. The aim of the study was to characterize the gene and protein expression of the TOB/BTG family in intestinal tissue of patients with IBD. This is an observational and cross-sectional study that included 63 IBD patients. Gene expression of TOB/BTG family was measured by RT-PCR. Protein expression of TOB/CD16 and BTG/Ki-67 was evaluated by immunohistochemistry. TOB/BTG family mRNAs were detected and quantitated by RT-qPCR in rectal and ileum biopsies from UC patients and CD patients, respectively, and non-inflammatory control tissues. Results showed that TOB1 and BTG1 gene expression was decreased in the colonic mucosa from patients with UC compared with the control group. The TOB2 and BTG2 genes were over-expressed in the colonic mucosa of patients with UC in remission compared with the active UC and control group. The high TOB2 gene expression was associated with histological remission (P = .01). TOB1/CD16, TOB2/CD16, BTG1/Ki-67, BTG2/Ki-67 and BTG4/Ki-67 single and double positive cells were mostly NK, macrophages, epithelial cells, connective tissue cells and perivascular inflammatory infiltrates in tissues from patients with UC and CD. 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subjects Adult
Cell Proliferation - physiology
Colitis - metabolism
Colon - metabolism
Cross-Sectional Studies
Epithelial Cells - metabolism
Female
Gene Expression - physiology
Humans
Immediate-Early Proteins - metabolism
Inflammatory Bowel Diseases - metabolism
Intestinal Mucosa - metabolism
Ki-67 Antigen - metabolism
Macrophages - metabolism
Male
Middle Aged
Receptors, IgG - metabolism
RNA, Messenger - metabolism
Tumor Suppressor Proteins - metabolism
title Expression of TOB/BTG family members in patients with inflammatory bowel disease
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