Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation

Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-elec...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2020-12, Vol.370 (6523)
Hauptverfasser:	Townsend, Cole, Leelaram, Majety N, Agafonov, Dmitry E, Dybkov, Olexandr, Will, Cindy L, Bertram, Karl, Urlaub, Henning, Kastner, Berthold, Stark, Holger, Lührmann, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Assembly Binding sites Catalytic Domain Cryoelectron Microscopy Electron microscopy Folding Humans Intermediates Kinases Microscopy Molecular structure mRNA Networks Precursors Protein Conformation Protein folding Proteins Ribonucleoproteins (U2 small nuclear) RNA Folding RNA Splicing RNA, Catalytic - chemistry RNA, Small Nuclear - chemistry RNA, Small Nuclear - genetics RNA-Binding Proteins - chemistry Scaffolds snRNA Spliceosomes - chemistry Spliceosomes - genetics Splicing Transmission electron microscopy
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