RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)
Rhabosciadium aucheri is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD)...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2020-12, Vol.47 (12), p.9345-9352 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9352 |
|---|---|
| container_issue | 12 |
| container_start_page | 9345 |
| container_title | Molecular biology reports |
| container_volume | 47 |
| creator | Kazemeini, Fatemeh Asri, Younes Mostafavi, Golaleh Kalvandi, Ramezan Mehregan, Iraj |
| description | Rhabosciadium aucheri
is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon’s index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation. |
| doi_str_mv | 10.1007/s11033-020-06028-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2465435175</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2465435175</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-f32d51f7d82c11eaf5ca2ebf5594aa83aef82c2fbdbdb6b6339f1766758399753</originalsourceid><addsrcrecordid>eNp9kU1r3DAQhkVpodu0f6AnQS_pQak-LMs-btMkDQRaQnIWY3m0q2BLrmQHcu8Pj7NbKPRQ5jCHed6XgYeQj4KfCc7NlyIEV4pxyRmvuWxY84pshDaKVa1pXpMNV1ywqtHiLXlXygPnvBJGb8jv2-3Pb6yDgj3FRxgWmEOKNHm6w4hzcLQPj5hLmJ8ojCnu6JSmZThQ5QWb90ivM8QAkWLscVwjZUIXsNDbPXR9Ki5AH5aRwuL2mAP9mkIpZ_R0OwVwCPj5PXnjYSj44c8-IfeXF3fn39nNj6vr8-0Nc0rLmXkley286RvphEDw2oHEzmvdVgCNAvTrRfquX6fuaqVaL0xdG92otjVanZDTY--U068Fy2zHUBwOA0RMS7GyqnWltDign_5BH9KS4_qdldoYU1W1USslj5TLqZSM3k45jJCfrOD2RYw9irGrGHsQY5s1pI6hssJxh_lv9X9Szybwkdw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2577744673</pqid></control><display><type>article</type><title>RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)</title><source>SpringerNature Journals</source><creator>Kazemeini, Fatemeh ; Asri, Younes ; Mostafavi, Golaleh ; Kalvandi, Ramezan ; Mehregan, Iraj</creator><creatorcontrib>Kazemeini, Fatemeh ; Asri, Younes ; Mostafavi, Golaleh ; Kalvandi, Ramezan ; Mehregan, Iraj</creatorcontrib><description>Rhabosciadium aucheri
is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon’s index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-020-06028-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Endemic species ; Gene flow ; Genetic distance ; Genetic diversity ; Geographical distribution ; Histology ; Life Sciences ; Morphology ; Original Article ; Polymerase chain reaction ; Polymorphism ; Population genetics ; Random amplified polymorphic DNA</subject><ispartof>Molecular biology reports, 2020-12, Vol.47 (12), p.9345-9352</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-f32d51f7d82c11eaf5ca2ebf5594aa83aef82c2fbdbdb6b6339f1766758399753</citedby><cites>FETCH-LOGICAL-c352t-f32d51f7d82c11eaf5ca2ebf5594aa83aef82c2fbdbdb6b6339f1766758399753</cites><orcidid>0000-0002-1908-7076</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-020-06028-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-020-06028-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Kazemeini, Fatemeh</creatorcontrib><creatorcontrib>Asri, Younes</creatorcontrib><creatorcontrib>Mostafavi, Golaleh</creatorcontrib><creatorcontrib>Kalvandi, Ramezan</creatorcontrib><creatorcontrib>Mehregan, Iraj</creatorcontrib><title>RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Rhabosciadium aucheri
is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon’s index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Endemic species</subject><subject>Gene flow</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Geographical distribution</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Population genetics</subject><subject>Random amplified polymorphic DNA</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1r3DAQhkVpodu0f6AnQS_pQak-LMs-btMkDQRaQnIWY3m0q2BLrmQHcu8Pj7NbKPRQ5jCHed6XgYeQj4KfCc7NlyIEV4pxyRmvuWxY84pshDaKVa1pXpMNV1ywqtHiLXlXygPnvBJGb8jv2-3Pb6yDgj3FRxgWmEOKNHm6w4hzcLQPj5hLmJ8ojCnu6JSmZThQ5QWb90ivM8QAkWLscVwjZUIXsNDbPXR9Ki5AH5aRwuL2mAP9mkIpZ_R0OwVwCPj5PXnjYSj44c8-IfeXF3fn39nNj6vr8-0Nc0rLmXkley286RvphEDw2oHEzmvdVgCNAvTrRfquX6fuaqVaL0xdG92otjVanZDTY--U068Fy2zHUBwOA0RMS7GyqnWltDign_5BH9KS4_qdldoYU1W1USslj5TLqZSM3k45jJCfrOD2RYw9irGrGHsQY5s1pI6hssJxh_lv9X9Szybwkdw</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Kazemeini, Fatemeh</creator><creator>Asri, Younes</creator><creator>Mostafavi, Golaleh</creator><creator>Kalvandi, Ramezan</creator><creator>Mehregan, Iraj</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1908-7076</orcidid></search><sort><creationdate>20201201</creationdate><title>RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)</title><author>Kazemeini, Fatemeh ; Asri, Younes ; Mostafavi, Golaleh ; Kalvandi, Ramezan ; Mehregan, Iraj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-f32d51f7d82c11eaf5ca2ebf5594aa83aef82c2fbdbdb6b6339f1766758399753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Endemic species</topic><topic>Gene flow</topic><topic>Genetic distance</topic><topic>Genetic diversity</topic><topic>Geographical distribution</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Population genetics</topic><topic>Random amplified polymorphic DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kazemeini, Fatemeh</creatorcontrib><creatorcontrib>Asri, Younes</creatorcontrib><creatorcontrib>Mostafavi, Golaleh</creatorcontrib><creatorcontrib>Kalvandi, Ramezan</creatorcontrib><creatorcontrib>Mehregan, Iraj</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kazemeini, Fatemeh</au><au>Asri, Younes</au><au>Mostafavi, Golaleh</au><au>Kalvandi, Ramezan</au><au>Mehregan, Iraj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><date>2020-12-01</date><risdate>2020</risdate><volume>47</volume><issue>12</issue><spage>9345</spage><epage>9352</epage><pages>9345-9352</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Rhabosciadium aucheri
is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon’s index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11033-020-06028-8</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1908-7076</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2020-12, Vol.47 (12), p.9345-9352 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2465435175 |
| source | SpringerNature Journals |
| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Endemic species Gene flow Genetic distance Genetic diversity Geographical distribution Histology Life Sciences Morphology Original Article Polymerase chain reaction Polymorphism Population genetics Random amplified polymorphic DNA |
| title | RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T17%3A54%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RAPD-based%20evaluation%20of%20genetic%20diversity%20among%20populations%20of%20the%20Iranian%20endemic%20species%20Rhabdosciadium%20aucheri%20Boiss.%20(Apiaceae)&rft.jtitle=Molecular%20biology%20reports&rft.au=Kazemeini,%20Fatemeh&rft.date=2020-12-01&rft.volume=47&rft.issue=12&rft.spage=9345&rft.epage=9352&rft.pages=9345-9352&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-020-06028-8&rft_dat=%3Cproquest_cross%3E2465435175%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2577744673&rft_id=info:pmid/&rfr_iscdi=true |