The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury
Background There are only a few reports evaluating the applicability of endothelial-damage markers analysis by immunohistochemistry (IHC) in kidney allograft samples. This study analyzed the expression of Caveolin-1 (Cav), von Willebrand factor (Vwf), and T-cadherin (Cad) in kidney biopsies and thei...
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| Veröffentlicht in: | Clinical and experimental nephrology 2021-03, Vol.25 (3), p.305-314 |
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| creator | Teixeira, André Costa Távora, Fábio de Deus e Silva, Melissa Lou Fagundes Prado, Renan Martins Gomes de Matos Esmeraldo, Ronaldo de Sandes-Freitas, Tainá Veras |
| description | Background
There are only a few reports evaluating the applicability of endothelial-damage markers analysis by immunohistochemistry (IHC) in kidney allograft samples. This study analyzed the expression of Caveolin-1 (Cav), von Willebrand factor (Vwf), and T-cadherin (Cad) in kidney biopsies and their association with antibody-mediated injury.
Methods
In this retrospective study, 114 cases with antibody-mediated changes (Banff, 2020) and 72 with interstitial fibrosis/tubular atrophy were selected. IHC for Cav, Vwf and Cad was performed and evaluated according to their qualitative expression in peritubular capillaries. The cases were grouped according to the presence of microvascular inflammation (MVI), donor-specific antibodies (DSA), C4d positivity and antibody-mediated rejection (AMR). A level of significance |
| doi_str_mv | 10.1007/s10157-020-01994-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2464606098</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2495194584</sourcerecordid><originalsourceid>FETCH-LOGICAL-c454t-7c4fb58cf7998233fb45bfe073effaaea90d958b4480212887bcf650fcff5e983</originalsourceid><addsrcrecordid>eNp9kc2OFCEUhYnROOPoC7gwJG5cDApVUAVL0_EvmcRNG5cEqMsUbRW0UDVjP42vKtM9auLC1b3hfufA5SD0nNHXjNL-TWGUiZ7QhhLKlOKke4DOGW970vdKPax9yxvCesHO0JNSdpRSqYR6jM7atuENE905-rkdAYd5XmMaQ1mSG2EOzkwYfuwzlBJSxMnjm1q-hmkCm00csDduSfkSb4kzwwg5xEt8d74xN5CmEAnDoeAQXQZTYKgd_haGCAdspildZ-MXbEPalwAF34ZlrOol2DQcyAxDMMtRs1vz4Sl65M1U4Nl9vUBf3r_bbj6Sq88fPm3eXhHHBV9I77i3QjpfF5dN23rLhfVA-xa8NwaMooMS0nIuacMaKXvrfCeod94LULK9QK9Ovvucvq9QFj2H4mCaTIS0Ft3wjne0o0f05T_oLq051tdVSgmmuJC8Us2JcjmVksHrfQ6zyQfNqL6LT5_i0zU-fYxPd1X04t56tfUj_kh-51WB9gSUOorXkP_e_R_bX6_LqI4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2495194584</pqid></control><display><type>article</type><title>The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury</title><source>SpringerLink</source><creator>Teixeira, André Costa ; Távora, Fábio ; de Deus e Silva, Melissa Lou Fagundes ; Prado, Renan Martins Gomes ; de Matos Esmeraldo, Ronaldo ; de Sandes-Freitas, Tainá Veras</creator><creatorcontrib>Teixeira, André Costa ; Távora, Fábio ; de Deus e Silva, Melissa Lou Fagundes ; Prado, Renan Martins Gomes ; de Matos Esmeraldo, Ronaldo ; de Sandes-Freitas, Tainá Veras</creatorcontrib><description><![CDATA[Background
There are only a few reports evaluating the applicability of endothelial-damage markers analysis by immunohistochemistry (IHC) in kidney allograft samples. This study analyzed the expression of Caveolin-1 (Cav), von Willebrand factor (Vwf), and T-cadherin (Cad) in kidney biopsies and their association with antibody-mediated injury.
Methods
In this retrospective study, 114 cases with antibody-mediated changes (Banff, 2020) and 72 with interstitial fibrosis/tubular atrophy were selected. IHC for Cav, Vwf and Cad was performed and evaluated according to their qualitative expression in peritubular capillaries. The cases were grouped according to the presence of microvascular inflammation (MVI), donor-specific antibodies (DSA), C4d positivity and antibody-mediated rejection (AMR). A level of significance < 0.05 was adopted.
Results
Vwf expression was associated with MVI (
p
< 0.001), DSA (
p
= 0.016), C4d (
p
< 0.001) and AMR (
p
< 0.001), and was higher in DSA+/C4d+ cases despite MVI (
p
< 0.001). The expression of Cad correlated with MVI (
p
= 0.015), C4d (
p
= 0.005) and AMR (
p
= < 0.001). Cad was more expressed in chronic AMR compared with acute/active cases (
p
= 0.001). Cav expression was associated with MVI (
p
= 0.029) and AMR (
p
= 0.016) and was also higher in chronic AMR (
p
= 0.049). A combined score of Vwf and Cad was higher in AMR when compared with C4d without rejection and IF/TA cases (
p
< 0.001).
Conclusion
Vwf, Cad and Cav expression shows association with antibody-mediated injury and may be helpful to support AMR diagnosis.]]></description><identifier>ISSN: 1342-1751</identifier><identifier>EISSN: 1437-7799</identifier><identifier>DOI: 10.1007/s10157-020-01994-6</identifier><identifier>PMID: 33242156</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Antibodies ; Atrophy ; Biopsy ; Cadherins ; Capillaries ; Caveolin ; Caveolin-1 ; Fibrosis ; Graft rejection ; Immunohistochemistry ; Inflammation ; Kidney transplantation ; Medicine ; Medicine & Public Health ; Microvasculature ; Nephrology ; Original Article ; T-cadherin ; Urology ; Von Willebrand factor</subject><ispartof>Clinical and experimental nephrology, 2021-03, Vol.25 (3), p.305-314</ispartof><rights>Japanese Society of Nephrology 2020</rights><rights>Japanese Society of Nephrology 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-7c4fb58cf7998233fb45bfe073effaaea90d958b4480212887bcf650fcff5e983</citedby><cites>FETCH-LOGICAL-c454t-7c4fb58cf7998233fb45bfe073effaaea90d958b4480212887bcf650fcff5e983</cites><orcidid>0000-0002-9648-3507</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10157-020-01994-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10157-020-01994-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33242156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teixeira, André Costa</creatorcontrib><creatorcontrib>Távora, Fábio</creatorcontrib><creatorcontrib>de Deus e Silva, Melissa Lou Fagundes</creatorcontrib><creatorcontrib>Prado, Renan Martins Gomes</creatorcontrib><creatorcontrib>de Matos Esmeraldo, Ronaldo</creatorcontrib><creatorcontrib>de Sandes-Freitas, Tainá Veras</creatorcontrib><title>The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury</title><title>Clinical and experimental nephrology</title><addtitle>Clin Exp Nephrol</addtitle><addtitle>Clin Exp Nephrol</addtitle><description><![CDATA[Background
There are only a few reports evaluating the applicability of endothelial-damage markers analysis by immunohistochemistry (IHC) in kidney allograft samples. This study analyzed the expression of Caveolin-1 (Cav), von Willebrand factor (Vwf), and T-cadherin (Cad) in kidney biopsies and their association with antibody-mediated injury.
Methods
In this retrospective study, 114 cases with antibody-mediated changes (Banff, 2020) and 72 with interstitial fibrosis/tubular atrophy were selected. IHC for Cav, Vwf and Cad was performed and evaluated according to their qualitative expression in peritubular capillaries. The cases were grouped according to the presence of microvascular inflammation (MVI), donor-specific antibodies (DSA), C4d positivity and antibody-mediated rejection (AMR). A level of significance < 0.05 was adopted.
Results
Vwf expression was associated with MVI (
p
< 0.001), DSA (
p
= 0.016), C4d (
p
< 0.001) and AMR (
p
< 0.001), and was higher in DSA+/C4d+ cases despite MVI (
p
< 0.001). The expression of Cad correlated with MVI (
p
= 0.015), C4d (
p
= 0.005) and AMR (
p
= < 0.001). Cad was more expressed in chronic AMR compared with acute/active cases (
p
= 0.001). Cav expression was associated with MVI (
p
= 0.029) and AMR (
p
= 0.016) and was also higher in chronic AMR (
p
= 0.049). A combined score of Vwf and Cad was higher in AMR when compared with C4d without rejection and IF/TA cases (
p
< 0.001).
Conclusion
Vwf, Cad and Cav expression shows association with antibody-mediated injury and may be helpful to support AMR diagnosis.]]></description><subject>Antibodies</subject><subject>Atrophy</subject><subject>Biopsy</subject><subject>Cadherins</subject><subject>Capillaries</subject><subject>Caveolin</subject><subject>Caveolin-1</subject><subject>Fibrosis</subject><subject>Graft rejection</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Kidney transplantation</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microvasculature</subject><subject>Nephrology</subject><subject>Original Article</subject><subject>T-cadherin</subject><subject>Urology</subject><subject>Von Willebrand factor</subject><issn>1342-1751</issn><issn>1437-7799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc2OFCEUhYnROOPoC7gwJG5cDApVUAVL0_EvmcRNG5cEqMsUbRW0UDVjP42vKtM9auLC1b3hfufA5SD0nNHXjNL-TWGUiZ7QhhLKlOKke4DOGW970vdKPax9yxvCesHO0JNSdpRSqYR6jM7atuENE905-rkdAYd5XmMaQ1mSG2EOzkwYfuwzlBJSxMnjm1q-hmkCm00csDduSfkSb4kzwwg5xEt8d74xN5CmEAnDoeAQXQZTYKgd_haGCAdspildZ-MXbEPalwAF34ZlrOol2DQcyAxDMMtRs1vz4Sl65M1U4Nl9vUBf3r_bbj6Sq88fPm3eXhHHBV9I77i3QjpfF5dN23rLhfVA-xa8NwaMooMS0nIuacMaKXvrfCeod94LULK9QK9Ovvucvq9QFj2H4mCaTIS0Ft3wjne0o0f05T_oLq051tdVSgmmuJC8Us2JcjmVksHrfQ6zyQfNqL6LT5_i0zU-fYxPd1X04t56tfUj_kh-51WB9gSUOorXkP_e_R_bX6_LqI4</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Teixeira, André Costa</creator><creator>Távora, Fábio</creator><creator>de Deus e Silva, Melissa Lou Fagundes</creator><creator>Prado, Renan Martins Gomes</creator><creator>de Matos Esmeraldo, Ronaldo</creator><creator>de Sandes-Freitas, Tainá Veras</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9648-3507</orcidid></search><sort><creationdate>20210301</creationdate><title>The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury</title><author>Teixeira, André Costa ; Távora, Fábio ; de Deus e Silva, Melissa Lou Fagundes ; Prado, Renan Martins Gomes ; de Matos Esmeraldo, Ronaldo ; de Sandes-Freitas, Tainá Veras</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-7c4fb58cf7998233fb45bfe073effaaea90d958b4480212887bcf650fcff5e983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Atrophy</topic><topic>Biopsy</topic><topic>Cadherins</topic><topic>Capillaries</topic><topic>Caveolin</topic><topic>Caveolin-1</topic><topic>Fibrosis</topic><topic>Graft rejection</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Kidney transplantation</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microvasculature</topic><topic>Nephrology</topic><topic>Original Article</topic><topic>T-cadherin</topic><topic>Urology</topic><topic>Von Willebrand factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teixeira, André Costa</creatorcontrib><creatorcontrib>Távora, Fábio</creatorcontrib><creatorcontrib>de Deus e Silva, Melissa Lou Fagundes</creatorcontrib><creatorcontrib>Prado, Renan Martins Gomes</creatorcontrib><creatorcontrib>de Matos Esmeraldo, Ronaldo</creatorcontrib><creatorcontrib>de Sandes-Freitas, Tainá Veras</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, André Costa</au><au>Távora, Fábio</au><au>de Deus e Silva, Melissa Lou Fagundes</au><au>Prado, Renan Martins Gomes</au><au>de Matos Esmeraldo, Ronaldo</au><au>de Sandes-Freitas, Tainá Veras</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury</atitle><jtitle>Clinical and experimental nephrology</jtitle><stitle>Clin Exp Nephrol</stitle><addtitle>Clin Exp Nephrol</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>25</volume><issue>3</issue><spage>305</spage><epage>314</epage><pages>305-314</pages><issn>1342-1751</issn><eissn>1437-7799</eissn><abstract><![CDATA[Background
There are only a few reports evaluating the applicability of endothelial-damage markers analysis by immunohistochemistry (IHC) in kidney allograft samples. This study analyzed the expression of Caveolin-1 (Cav), von Willebrand factor (Vwf), and T-cadherin (Cad) in kidney biopsies and their association with antibody-mediated injury.
Methods
In this retrospective study, 114 cases with antibody-mediated changes (Banff, 2020) and 72 with interstitial fibrosis/tubular atrophy were selected. IHC for Cav, Vwf and Cad was performed and evaluated according to their qualitative expression in peritubular capillaries. The cases were grouped according to the presence of microvascular inflammation (MVI), donor-specific antibodies (DSA), C4d positivity and antibody-mediated rejection (AMR). A level of significance < 0.05 was adopted.
Results
Vwf expression was associated with MVI (
p
< 0.001), DSA (
p
= 0.016), C4d (
p
< 0.001) and AMR (
p
< 0.001), and was higher in DSA+/C4d+ cases despite MVI (
p
< 0.001). The expression of Cad correlated with MVI (
p
= 0.015), C4d (
p
= 0.005) and AMR (
p
= < 0.001). Cad was more expressed in chronic AMR compared with acute/active cases (
p
= 0.001). Cav expression was associated with MVI (
p
= 0.029) and AMR (
p
= 0.016) and was also higher in chronic AMR (
p
= 0.049). A combined score of Vwf and Cad was higher in AMR when compared with C4d without rejection and IF/TA cases (
p
< 0.001).
Conclusion
Vwf, Cad and Cav expression shows association with antibody-mediated injury and may be helpful to support AMR diagnosis.]]></abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>33242156</pmid><doi>10.1007/s10157-020-01994-6</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9648-3507</orcidid></addata></record> |
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| ispartof | Clinical and experimental nephrology, 2021-03, Vol.25 (3), p.305-314 |
| issn | 1342-1751 1437-7799 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2464606098 |
| source | SpringerLink |
| subjects | Antibodies Atrophy Biopsy Cadherins Capillaries Caveolin Caveolin-1 Fibrosis Graft rejection Immunohistochemistry Inflammation Kidney transplantation Medicine Medicine & Public Health Microvasculature Nephrology Original Article T-cadherin Urology Von Willebrand factor |
| title | The immunohistochemical expression of von Willebrand factor, T-cadherin, and Caveolin-1 is increased in kidney allograft biopsies with antibody-mediated injury |
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