Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns
Purpose To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model. Methods After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2021-04, Vol.259 (4), p.929-940 |
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| creator | Zhang, Nuan Luo, Xiaohui Zhang, Shiyao Liu, Ren Liang, Lingyi Su, Wenru Liang, Dan |
| description | Purpose
To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.
Methods
After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF‐α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.
Results
TNF‐α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in
vitro
. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.
Conclusion
Subconjunctival injection of TNF‐α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression. |
| doi_str_mv | 10.1007/s00417-020-05017-8 |
| format | Article |
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To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.
Methods
After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF‐α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.
Results
TNF‐α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in
vitro
. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.
Conclusion
Subconjunctival injection of TNF‐α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-020-05017-8</identifier><identifier>PMID: 33237391</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Anti-Inflammatory Agents - therapeutic use ; Basic Science ; Bone Marrow ; Burns ; Burns, Chemical - drug therapy ; Cornea ; Fibrosis ; Filter paper ; Indomethacin ; Inflammation ; Injection ; Macrophages ; Medicine ; Medicine & Public Health ; Mesenchymal Stem Cells ; Necrosis ; Ophthalmology ; Rats ; Sodium hydroxide ; Stem cell transplantation ; Stem cells ; Tumor Necrosis Factor-alpha ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2021-04, Vol.259 (4), p.929-940</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-5ab712361411c7b2bee1ba0aeb40c5c8290e2f53eacd9537cb591c1f8c5256e63</citedby><cites>FETCH-LOGICAL-c375t-5ab712361411c7b2bee1ba0aeb40c5c8290e2f53eacd9537cb591c1f8c5256e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-020-05017-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-020-05017-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33237391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Nuan</creatorcontrib><creatorcontrib>Luo, Xiaohui</creatorcontrib><creatorcontrib>Zhang, Shiyao</creatorcontrib><creatorcontrib>Liu, Ren</creatorcontrib><creatorcontrib>Liang, Lingyi</creatorcontrib><creatorcontrib>Su, Wenru</creatorcontrib><creatorcontrib>Liang, Dan</creatorcontrib><title>Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Purpose
To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.
Methods
After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF‐α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.
Results
TNF‐α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in
vitro
. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.
Conclusion
Subconjunctival injection of TNF‐α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>Basic Science</subject><subject>Bone Marrow</subject><subject>Burns</subject><subject>Burns, Chemical - drug therapy</subject><subject>Cornea</subject><subject>Fibrosis</subject><subject>Filter paper</subject><subject>Indomethacin</subject><subject>Inflammation</subject><subject>Injection</subject><subject>Macrophages</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal Stem Cells</subject><subject>Necrosis</subject><subject>Ophthalmology</subject><subject>Rats</subject><subject>Sodium hydroxide</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Tumor Necrosis Factor-alpha</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kc-OFCEQxonRuOPqC3gwJF68oAU0Q8_RbPyXbOJBTfZGgK52GRsYoXvNPpbxPXwmme1VEw-eqMCvvq-oj5DHHJ5zAP2iAnRcMxDAQEGr-jtkwzupmAZxcZdsQAvOeikuTsiDWvfQeKn4fXIipZBa7viG_PiwOJ_Tfkl-Dld2oiHtsZU50TzSeYm50IS-5BoqHa2fc2E_v9NDQVbnEJfJzjhQlxPSaEvJ39iAJVy1u4gVk7-8jk20zhipx2mqFNOlTR4rtWkOLKRxsjHaG0ObhvV2DG41DG0K3zwKtdMXOwXqlpLqQ3JvtFPFR7fnKfn0-tXHs7fs_P2bd2cvz5mXWs1MWae5kFvece61Ew6ROwsWXQde-V7sAMWoJFo_7JTU3qkd93zsvRJqi1t5Sp6tuoeSvy5YZxNDPf7CJsxLNaLbdnzXSTiiT_9B97mN2qYzQoHuoUlDo8RKHfdZC47mUEJb27XhYI6RmjVS0yI1N5GavjU9uZVeXMThT8vvDBsgV6C2p_QZy1_v_8j-As4lsb0</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Zhang, Nuan</creator><creator>Luo, Xiaohui</creator><creator>Zhang, Shiyao</creator><creator>Liu, Ren</creator><creator>Liang, Lingyi</creator><creator>Su, Wenru</creator><creator>Liang, Dan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20210401</creationdate><title>Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns</title><author>Zhang, Nuan ; Luo, Xiaohui ; Zhang, Shiyao ; Liu, Ren ; Liang, Lingyi ; Su, Wenru ; Liang, Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-5ab712361411c7b2bee1ba0aeb40c5c8290e2f53eacd9537cb591c1f8c5256e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Basic Science</topic><topic>Bone Marrow</topic><topic>Burns</topic><topic>Burns, Chemical - drug therapy</topic><topic>Cornea</topic><topic>Fibrosis</topic><topic>Filter paper</topic><topic>Indomethacin</topic><topic>Inflammation</topic><topic>Injection</topic><topic>Macrophages</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal Stem Cells</topic><topic>Necrosis</topic><topic>Ophthalmology</topic><topic>Rats</topic><topic>Sodium hydroxide</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Tumor Necrosis Factor-alpha</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Nuan</creatorcontrib><creatorcontrib>Luo, Xiaohui</creatorcontrib><creatorcontrib>Zhang, Shiyao</creatorcontrib><creatorcontrib>Liu, Ren</creatorcontrib><creatorcontrib>Liang, Lingyi</creatorcontrib><creatorcontrib>Su, Wenru</creatorcontrib><creatorcontrib>Liang, Dan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection (ProQuest Medical & Health Databases)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Nuan</au><au>Luo, Xiaohui</au><au>Zhang, Shiyao</au><au>Liu, Ren</au><au>Liang, Lingyi</au><au>Su, Wenru</au><au>Liang, Dan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>259</volume><issue>4</issue><spage>929</spage><epage>940</epage><pages>929-940</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Purpose
To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.
Methods
After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF‐α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.
Results
TNF‐α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in
vitro
. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.
Conclusion
Subconjunctival injection of TNF‐α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33237391</pmid><doi>10.1007/s00417-020-05017-8</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-832X |
| ispartof | Graefe's archive for clinical and experimental ophthalmology, 2021-04, Vol.259 (4), p.929-940 |
| issn | 0721-832X 1435-702X |
| language | eng |
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| source | MEDLINE; SpringerLink |
| subjects | Animals Anti-Inflammatory Agents - therapeutic use Basic Science Bone Marrow Burns Burns, Chemical - drug therapy Cornea Fibrosis Filter paper Indomethacin Inflammation Injection Macrophages Medicine Medicine & Public Health Mesenchymal Stem Cells Necrosis Ophthalmology Rats Sodium hydroxide Stem cell transplantation Stem cells Tumor Necrosis Factor-alpha Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns |
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