Muscle fiber capillarization is associated with various indices of skeletal muscle mass in healthy, older men

Muscle fiber capillarization is associated with various indices of skeletal muscle mass in healthy, older men

Muscle fiber capillarization plays a fundamental role in the regulation of skeletal muscle mass maintenance. However, it remains unclear to what extent capillarization is related to various other skeletal muscle characteristics. In this study we determined whether muscle fiber capillarization is ind...

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Veröffentlicht in:Experimental gerontology 2021-01, Vol.143, p.111161-111161, Article 111161
Hauptverfasser: Betz, M.W., Aussieker, T., Kruger, C.Q., Gorissen, S.H.M., van Loon, L.J.C., Snijders, T.
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Fractional synthetic rate
Lean body mass
Muscle perfusion
Sarcopenia
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container_start_page 111161
container_title Experimental gerontology
container_volume 143
creator Betz, M.W.
Aussieker, T.
Kruger, C.Q.
Gorissen, S.H.M.
van Loon, L.J.C.
Snijders, T.
description Muscle fiber capillarization plays a fundamental role in the regulation of skeletal muscle mass maintenance. However, it remains unclear to what extent capillarization is related to various other skeletal muscle characteristics. In this study we determined whether muscle fiber capillarization is independently associated with measures of skeletal muscle mass, both on a whole-body and cellular level, and post-absorptive muscle protein synthesis rates in healthy older men. Forty-six healthy older (70 ± 4 y) men participated in a trial during which basal muscle protein synthesis rates were assessed using stable isotope tracer methodology. Blood and muscle biopsy samples were collected to assess post-absorptive muscle protein synthesis rates over a 3-hour period. Immunohistochemistry was performed to determine various indices of muscle fiber capillarization, size, type distribution, and myonuclear content/domain size. Dual energy x-ray absorptiometry scans were performed to determine whole-body and appendicular lean tissue mass. Capillary-to-fiber ratio (C/Fi) and perimeter exchange (CFPE) index correlated with whole-body lean tissue mass (r = 0.43, P 
doi_str_mv 10.1016/j.exger.2020.111161
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However, it remains unclear to what extent capillarization is related to various other skeletal muscle characteristics. In this study we determined whether muscle fiber capillarization is independently associated with measures of skeletal muscle mass, both on a whole-body and cellular level, and post-absorptive muscle protein synthesis rates in healthy older men. Forty-six healthy older (70 ± 4 y) men participated in a trial during which basal muscle protein synthesis rates were assessed using stable isotope tracer methodology. Blood and muscle biopsy samples were collected to assess post-absorptive muscle protein synthesis rates over a 3-hour period. Immunohistochemistry was performed to determine various indices of muscle fiber capillarization, size, type distribution, and myonuclear content/domain size. Dual energy x-ray absorptiometry scans were performed to determine whole-body and appendicular lean tissue mass. Capillary-to-fiber ratio (C/Fi) and perimeter exchange (CFPE) index correlated with whole-body lean tissue mass (r = 0.43, P < 0.01 and r = 0.25, P < 0.10, respectively), appendicular lean tissue mass (r = 0.52, P < 0.001 and r = 0.37, P < 0.05, respectively) as well as appendicular lean tissue mass divided by body mass index (r = 0.65, P < 0.001 and r = 0.62, P < 0.001, respectively). Muscle fiber size correlated with C/Fi (r = 0.45, P < 0.01), but not with CFPE index. No associations were observed between different indices of muscle fiber capillarization and post-absorptive muscle protein synthesis rates in healthy, older men. The present study provides further evidence that muscle fiber capillarization may be a critical factor in the regulation of skeletal muscle maintenance in healthy older men. •Muscle fiber capillarization is positively associated with whole-body indices of skeletal muscle mass in older men.•Muscle fiber capillarization is not associated with post-absorptive muscle protein synthesis rates in older men.•Muscle fiber capillarization may be an important factor for skeletal muscle mass maintenance with aging.•Muscle fiber capillarization could be considered as an interventional target to support healthy muscle aging.]]></description><identifier>ISSN: 0531-5565</identifier><identifier>EISSN: 1873-6815</identifier><identifier>DOI: 10.1016/j.exger.2020.111161</identifier><identifier>PMID: 33227401</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Fractional synthetic rate ; Lean body mass ; Muscle perfusion ; Sarcopenia</subject><ispartof>Experimental gerontology, 2021-01, Vol.143, p.111161-111161, Article 111161</ispartof><rights>2020 The Authors</rights><rights>Copyright © 2020 The Authors. 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However, it remains unclear to what extent capillarization is related to various other skeletal muscle characteristics. In this study we determined whether muscle fiber capillarization is independently associated with measures of skeletal muscle mass, both on a whole-body and cellular level, and post-absorptive muscle protein synthesis rates in healthy older men. Forty-six healthy older (70 ± 4 y) men participated in a trial during which basal muscle protein synthesis rates were assessed using stable isotope tracer methodology. Blood and muscle biopsy samples were collected to assess post-absorptive muscle protein synthesis rates over a 3-hour period. Immunohistochemistry was performed to determine various indices of muscle fiber capillarization, size, type distribution, and myonuclear content/domain size. Dual energy x-ray absorptiometry scans were performed to determine whole-body and appendicular lean tissue mass. 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The present study provides further evidence that muscle fiber capillarization may be a critical factor in the regulation of skeletal muscle maintenance in healthy older men. •Muscle fiber capillarization is positively associated with whole-body indices of skeletal muscle mass in older men.•Muscle fiber capillarization is not associated with post-absorptive muscle protein synthesis rates in older men.•Muscle fiber capillarization may be an important factor for skeletal muscle mass maintenance with aging.•Muscle fiber capillarization could be considered as an interventional target to support healthy muscle aging.]]></description><subject>Fractional synthetic rate</subject><subject>Lean body mass</subject><subject>Muscle perfusion</subject><subject>Sarcopenia</subject><issn>0531-5565</issn><issn>1873-6815</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EoqXwBUjISxak2LGdx4IFqnhJRWxgbTn2hLrkUeykUL4elxSWeDOS59w7MxehU0qmlNDkcjmFz1dw05jE4Se8hO6hMc1SFiUZFftoTASjkRCJGKEj75eEkCRm9BCNGIvjlBM6RvVj73UFuLQFOKzVylaVcvZLdbZtsPVYed9qqzow-MN2C7wO3bb32DbGavC4LbF_gwo6VeF68KqDJvTxAlTVLTYXuK1MMK-hOUYHpao8nOzqBL3c3jzP7qP5093D7HoeaU54F5WMx8xknCqaK5ITQ7MsF7pUSqcqFVlZJFwJAlmeFWnCc05Lk0Ie6CJlMSVsgs4H35Vr33vwnayt1xBOayAsL2OecMo5S0VA2YBq13rvoJQrZ2vlNpISuc1ZLuVPznKbsxxyDqqz3YC-qMH8aX6DDcDVAEA4c22D3GsLjQZjHehOmtb-O-AbibWQBA</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Betz, M.W.</creator><creator>Aussieker, T.</creator><creator>Kruger, C.Q.</creator><creator>Gorissen, S.H.M.</creator><creator>van Loon, L.J.C.</creator><creator>Snijders, T.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202101</creationdate><title>Muscle fiber capillarization is associated with various indices of skeletal muscle mass in healthy, older men</title><author>Betz, M.W. ; Aussieker, T. ; Kruger, C.Q. ; Gorissen, S.H.M. ; van Loon, L.J.C. ; Snijders, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-f3423d841a19a090d18895cfaac7a758fb64a50e898b764941fd7e9a19b732103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Fractional synthetic rate</topic><topic>Lean body mass</topic><topic>Muscle perfusion</topic><topic>Sarcopenia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Betz, M.W.</creatorcontrib><creatorcontrib>Aussieker, T.</creatorcontrib><creatorcontrib>Kruger, C.Q.</creatorcontrib><creatorcontrib>Gorissen, S.H.M.</creatorcontrib><creatorcontrib>van Loon, L.J.C.</creatorcontrib><creatorcontrib>Snijders, T.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental gerontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Betz, M.W.</au><au>Aussieker, T.</au><au>Kruger, C.Q.</au><au>Gorissen, S.H.M.</au><au>van Loon, L.J.C.</au><au>Snijders, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscle fiber capillarization is associated with various indices of skeletal muscle mass in healthy, older men</atitle><jtitle>Experimental gerontology</jtitle><addtitle>Exp Gerontol</addtitle><date>2021-01</date><risdate>2021</risdate><volume>143</volume><spage>111161</spage><epage>111161</epage><pages>111161-111161</pages><artnum>111161</artnum><issn>0531-5565</issn><eissn>1873-6815</eissn><abstract><![CDATA[Muscle fiber capillarization plays a fundamental role in the regulation of skeletal muscle mass maintenance. However, it remains unclear to what extent capillarization is related to various other skeletal muscle characteristics. In this study we determined whether muscle fiber capillarization is independently associated with measures of skeletal muscle mass, both on a whole-body and cellular level, and post-absorptive muscle protein synthesis rates in healthy older men. Forty-six healthy older (70 ± 4 y) men participated in a trial during which basal muscle protein synthesis rates were assessed using stable isotope tracer methodology. Blood and muscle biopsy samples were collected to assess post-absorptive muscle protein synthesis rates over a 3-hour period. Immunohistochemistry was performed to determine various indices of muscle fiber capillarization, size, type distribution, and myonuclear content/domain size. Dual energy x-ray absorptiometry scans were performed to determine whole-body and appendicular lean tissue mass. Capillary-to-fiber ratio (C/Fi) and perimeter exchange (CFPE) index correlated with whole-body lean tissue mass (r = 0.43, P < 0.01 and r = 0.25, P < 0.10, respectively), appendicular lean tissue mass (r = 0.52, P < 0.001 and r = 0.37, P < 0.05, respectively) as well as appendicular lean tissue mass divided by body mass index (r = 0.65, P < 0.001 and r = 0.62, P < 0.001, respectively). Muscle fiber size correlated with C/Fi (r = 0.45, P < 0.01), but not with CFPE index. No associations were observed between different indices of muscle fiber capillarization and post-absorptive muscle protein synthesis rates in healthy, older men. The present study provides further evidence that muscle fiber capillarization may be a critical factor in the regulation of skeletal muscle maintenance in healthy older men. •Muscle fiber capillarization is positively associated with whole-body indices of skeletal muscle mass in older men.•Muscle fiber capillarization is not associated with post-absorptive muscle protein synthesis rates in older men.•Muscle fiber capillarization may be an important factor for skeletal muscle mass maintenance with aging.•Muscle fiber capillarization could be considered as an interventional target to support healthy muscle aging.]]></abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>33227401</pmid><doi>10.1016/j.exger.2020.111161</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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subjects Fractional synthetic rate
Lean body mass
Muscle perfusion
Sarcopenia
title Muscle fiber capillarization is associated with various indices of skeletal muscle mass in healthy, older men
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