Characterization of a thermostable phytase from Bacillus licheniformis WHU and further stabilization of the enzyme through disulfide bond engineering

•A thermostable phytase PhyBL from Bacillus licheniformis WHU was identified and characterized.•A variant G197C/A358C with enhanced thermostability and proteolytic resistance was obtained by disulfide bond engineering.•The stabilization mechanism in the G197C/A358C variant was revealed by molecular...

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Veröffentlicht in:	Enzyme and microbial technology 2020-12, Vol.142, p.109679-109679, Article 109679
Hauptverfasser:	Zhang, Zhijie, Yang, Jian, Xie, Peijuan, Gao, Yanping, Bai, Jun, Zhang, Chun, Liu, Li, Wang, Qin, Gao, Xiaowei
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus licheniformis Disulfide bond engineering Enzyme Phytase Phytate Protein engineering Thermostability
Online-Zugang:	Volltext
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