Decreased expression of m6A demethylase FTO in ovarian aging
Purpose N6-methyladenosine (m 6 A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2021-05, Vol.303 (5), p.1363-1369 |
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| container_title | Archives of gynecology and obstetrics |
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| creator | Sun, Xiaoyan Zhang, Yigan Hu, Yuping An, Junxia Li, Lifei Wang, Yiqing Zhang, Xuehong |
| description | Purpose
N6-methyladenosine (m
6
A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level and the m
6
A content during ovarian aging.
Methods
The expression level of FTO and the content of m
6
A RNA methylation in human follicular fluid (FF), granulosa cells (GCs) and mouse ovary from different age groups were studied by ELISA, WB, qRT-PCR, IHC and m
6
A Colorimetric.
Results
Human FF ELISA quantified that the level of FTO protein decreased with age (
P
= 0.025). QRT-PCR results showed that the relative expression of FTO in human GCs was lower in the elderly group than in the young group (
P
= 0.012). FTO mRNA and protein expression levels were lower in the ovary of 32-week-old mice than in 3- and 8-week-old mice (
P
|
| doi_str_mv | 10.1007/s00404-020-05895-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2463606668</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2463606668</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1977-cf6e828dbe88929312012d491689e8f16676feb20ca65f2047a2842ae6cac9aa3</originalsourceid><addsrcrecordid>eNp9kE9LAzEQxYMoWKtfwNOCFy-rk-zuJAEvRa0KhV7qOaTZ2bpl_9SkFfvtTV1B8OBpBt7vvRkeY5ccbjiAvA0AOeQpCEihULpI5REb8TwTKUjOj9kI9GEHlKfsLIQ1ABdK4YjdPZDzZAOVCX1uPIVQ913SV0mLk6SklrZv-ybKyXQxT-qofFhf2y6xq7pbnbOTyjaBLn7mmL1OHxf3z-ls_vRyP5mljmspU1chKaHKJSmlhc64iNfLXHNUmlTFESVWtBTgLBaVgFxaoXJhCZ112tpszK6H3I3v33cUtqatg6OmsR31u2BEjhkCIqqIXv1B1_3Od_E7IwqeSQGZOFBioJzvQ_BUmY2vW-v3hoM5FGqGQk0s1HwXamQ0ZYMpRLhbkf-N_sf1BdRBdf8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2513720328</pqid></control><display><type>article</type><title>Decreased expression of m6A demethylase FTO in ovarian aging</title><source>SpringerLink Journals - AutoHoldings</source><creator>Sun, Xiaoyan ; Zhang, Yigan ; Hu, Yuping ; An, Junxia ; Li, Lifei ; Wang, Yiqing ; Zhang, Xuehong</creator><creatorcontrib>Sun, Xiaoyan ; Zhang, Yigan ; Hu, Yuping ; An, Junxia ; Li, Lifei ; Wang, Yiqing ; Zhang, Xuehong</creatorcontrib><description>Purpose
N6-methyladenosine (m
6
A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level and the m
6
A content during ovarian aging.
Methods
The expression level of FTO and the content of m
6
A RNA methylation in human follicular fluid (FF), granulosa cells (GCs) and mouse ovary from different age groups were studied by ELISA, WB, qRT-PCR, IHC and m
6
A Colorimetric.
Results
Human FF ELISA quantified that the level of FTO protein decreased with age (
P
= 0.025). QRT-PCR results showed that the relative expression of FTO in human GCs was lower in the elderly group than in the young group (
P
= 0.012). FTO mRNA and protein expression levels were lower in the ovary of 32-week-old mice than in 3- and 8-week-old mice (
P
< 0.05). Immunohistochemistry showed FTO was relatively decreased in 32-week-old mice (
P
< 0.05). The m
6
A content in total RNA from old human GCs and ovary from 32-week-old mice was significantly higher compared with the younger ones.
Conclusions
In human FF, GCs and mouse ovary, the expression of FTO decreased while the content of m
6
A increased with aging. However, the inner mechanism still needs further investigation.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-020-05895-7</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aging ; Endocrinology ; Gynecologic Endocrinology and Reproductive Medicine ; Gynecology ; Human Genetics ; Medicine ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Ovaries ; Proteins</subject><ispartof>Archives of gynecology and obstetrics, 2021-05, Vol.303 (5), p.1363-1369</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1977-cf6e828dbe88929312012d491689e8f16676feb20ca65f2047a2842ae6cac9aa3</citedby><cites>FETCH-LOGICAL-c1977-cf6e828dbe88929312012d491689e8f16676feb20ca65f2047a2842ae6cac9aa3</cites><orcidid>0000-0002-7216-0383</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-020-05895-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-020-05895-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Sun, Xiaoyan</creatorcontrib><creatorcontrib>Zhang, Yigan</creatorcontrib><creatorcontrib>Hu, Yuping</creatorcontrib><creatorcontrib>An, Junxia</creatorcontrib><creatorcontrib>Li, Lifei</creatorcontrib><creatorcontrib>Wang, Yiqing</creatorcontrib><creatorcontrib>Zhang, Xuehong</creatorcontrib><title>Decreased expression of m6A demethylase FTO in ovarian aging</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
N6-methyladenosine (m
6
A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level and the m
6
A content during ovarian aging.
Methods
The expression level of FTO and the content of m
6
A RNA methylation in human follicular fluid (FF), granulosa cells (GCs) and mouse ovary from different age groups were studied by ELISA, WB, qRT-PCR, IHC and m
6
A Colorimetric.
Results
Human FF ELISA quantified that the level of FTO protein decreased with age (
P
= 0.025). QRT-PCR results showed that the relative expression of FTO in human GCs was lower in the elderly group than in the young group (
P
= 0.012). FTO mRNA and protein expression levels were lower in the ovary of 32-week-old mice than in 3- and 8-week-old mice (
P
< 0.05). Immunohistochemistry showed FTO was relatively decreased in 32-week-old mice (
P
< 0.05). The m
6
A content in total RNA from old human GCs and ovary from 32-week-old mice was significantly higher compared with the younger ones.
Conclusions
In human FF, GCs and mouse ovary, the expression of FTO decreased while the content of m
6
A increased with aging. However, the inner mechanism still needs further investigation.</description><subject>Aging</subject><subject>Endocrinology</subject><subject>Gynecologic Endocrinology and Reproductive Medicine</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Ovaries</subject><subject>Proteins</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kE9LAzEQxYMoWKtfwNOCFy-rk-zuJAEvRa0KhV7qOaTZ2bpl_9SkFfvtTV1B8OBpBt7vvRkeY5ccbjiAvA0AOeQpCEihULpI5REb8TwTKUjOj9kI9GEHlKfsLIQ1ABdK4YjdPZDzZAOVCX1uPIVQ913SV0mLk6SklrZv-ybKyXQxT-qofFhf2y6xq7pbnbOTyjaBLn7mmL1OHxf3z-ls_vRyP5mljmspU1chKaHKJSmlhc64iNfLXHNUmlTFESVWtBTgLBaVgFxaoXJhCZ112tpszK6H3I3v33cUtqatg6OmsR31u2BEjhkCIqqIXv1B1_3Od_E7IwqeSQGZOFBioJzvQ_BUmY2vW-v3hoM5FGqGQk0s1HwXamQ0ZYMpRLhbkf-N_sf1BdRBdf8</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Sun, Xiaoyan</creator><creator>Zhang, Yigan</creator><creator>Hu, Yuping</creator><creator>An, Junxia</creator><creator>Li, Lifei</creator><creator>Wang, Yiqing</creator><creator>Zhang, Xuehong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7216-0383</orcidid></search><sort><creationdate>20210501</creationdate><title>Decreased expression of m6A demethylase FTO in ovarian aging</title><author>Sun, Xiaoyan ; Zhang, Yigan ; Hu, Yuping ; An, Junxia ; Li, Lifei ; Wang, Yiqing ; Zhang, Xuehong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1977-cf6e828dbe88929312012d491689e8f16676feb20ca65f2047a2842ae6cac9aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aging</topic><topic>Endocrinology</topic><topic>Gynecologic Endocrinology and Reproductive Medicine</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Ovaries</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Xiaoyan</creatorcontrib><creatorcontrib>Zhang, Yigan</creatorcontrib><creatorcontrib>Hu, Yuping</creatorcontrib><creatorcontrib>An, Junxia</creatorcontrib><creatorcontrib>Li, Lifei</creatorcontrib><creatorcontrib>Wang, Yiqing</creatorcontrib><creatorcontrib>Zhang, Xuehong</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Xiaoyan</au><au>Zhang, Yigan</au><au>Hu, Yuping</au><au>An, Junxia</au><au>Li, Lifei</au><au>Wang, Yiqing</au><au>Zhang, Xuehong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased expression of m6A demethylase FTO in ovarian aging</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><date>2021-05-01</date><risdate>2021</risdate><volume>303</volume><issue>5</issue><spage>1363</spage><epage>1369</epage><pages>1363-1369</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
N6-methyladenosine (m
6
A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level and the m
6
A content during ovarian aging.
Methods
The expression level of FTO and the content of m
6
A RNA methylation in human follicular fluid (FF), granulosa cells (GCs) and mouse ovary from different age groups were studied by ELISA, WB, qRT-PCR, IHC and m
6
A Colorimetric.
Results
Human FF ELISA quantified that the level of FTO protein decreased with age (
P
= 0.025). QRT-PCR results showed that the relative expression of FTO in human GCs was lower in the elderly group than in the young group (
P
= 0.012). FTO mRNA and protein expression levels were lower in the ovary of 32-week-old mice than in 3- and 8-week-old mice (
P
< 0.05). Immunohistochemistry showed FTO was relatively decreased in 32-week-old mice (
P
< 0.05). The m
6
A content in total RNA from old human GCs and ovary from 32-week-old mice was significantly higher compared with the younger ones.
Conclusions
In human FF, GCs and mouse ovary, the expression of FTO decreased while the content of m
6
A increased with aging. However, the inner mechanism still needs further investigation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00404-020-05895-7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7216-0383</orcidid></addata></record> |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Aging Endocrinology Gynecologic Endocrinology and Reproductive Medicine Gynecology Human Genetics Medicine Medicine & Public Health Obstetrics/Perinatology/Midwifery Ovaries Proteins |
| title | Decreased expression of m6A demethylase FTO in ovarian aging |
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