Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency
Background/Aim Biotin is a vital micronutrient that plays a role in metabolic homeostasis and the regulation of innate and adaptive immune system functions. Biotinidase deficiency (BTD) leads to impairment in biotin‐dependent immune functions. This study focused on immunophenotypic analysis of lymph...
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| Veröffentlicht in: | Pediatric allergy and immunology 2021-04, Vol.32 (3), p.586-598 |
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| creator | Karaoglan, Murat Nacarkahya, Gulper Keskin, Mehmet Keskin, Ozlem Atanaskovic‐Markovic, Marina |
| description | Background/Aim
Biotin is a vital micronutrient that plays a role in metabolic homeostasis and the regulation of innate and adaptive immune system functions. Biotinidase deficiency (BTD) leads to impairment in biotin‐dependent immune functions. This study focused on immunophenotypic analysis of lymphocyte subsets in newborns with BTD.
Patients and Methods
A total of 181 (95 female and 86 male; 114 had BTD and 67 were healthy) newborns underwent biotinidase enzyme activity, molecular and lymphocyte immunophenotyping analyses. BTD is classified into four biochemical phenotypes: profound, partial, heterozygous and normal. The following lymphocyte subsets were studied in all participants: total B lymphocyte (CD19), total T lymphocyte (CD3), helper T lymphocyte (CD3/CD4), cytotoxic T lymphocyte (CTL) (CD3/CD8), natural killer T lymphocyte (CD16/56) and a T‐lymphocyte activation marker (HLA‐DR).
Results
The percentages of lymphocyte subsets were similar in newborns with and without BTD. In all newborns with BTD, the mean CD3/CD4 levels were higher in females, while the CD3/CD8 levels were higher in males (P |
| doi_str_mv | 10.1111/pai.13416 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2463110458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2463110458</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3136-b2c10693650c2040795252f7cda5e144a22aa8f8a112f26f9ee2c157dcaf49d13</originalsourceid><addsrcrecordid>eNp10MFO3DAQBmALgcoWeugLIEtc2kPAY8fO5ohWtF1pJThQcYwcZ6w1SuwQJ1rl7TEN9IDEXObyzS_NT8h3YFeQ5rrX7gpEDuqIrECUZSaYWB-TFSuZzBTI4pR8jfGJMSiEgi_kVAgOBVNyRR63XTf50O_Rh3HunaHa63aOLtJgaTt3_T6YeUQapzriGKnz1OOhDoOP9ODGPa1dGJ13jY5IG7TOOPRmPicnVrcRv73tM_L31-3D5k-2u_u93dzsMiNAqKzmBpgqhZLMcJazopRccluYRkuEPNeca722aw3ALVe2REwXsmiMtnnZgDgjP5bcfgjPE8ax6lw02LbaY5hixXMlAFgu14lefqBPYRrSt0lJVnKe80Im9XNRZggxDmirfnCdHuYKWPXadpXarv61nezFW-JUd9j8l-_1JnC9gINrcf48qbq_2S6RL3efiSE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2509224275</pqid></control><display><type>article</type><title>Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency</title><source>Access via Wiley Online Library</source><creator>Karaoglan, Murat ; Nacarkahya, Gulper ; Keskin, Mehmet ; Keskin, Ozlem ; Atanaskovic‐Markovic, Marina</creator><contributor>Atanaskovic‐Markovic, Marina</contributor><creatorcontrib>Karaoglan, Murat ; Nacarkahya, Gulper ; Keskin, Mehmet ; Keskin, Ozlem ; Atanaskovic‐Markovic, Marina ; Atanaskovic‐Markovic, Marina</creatorcontrib><description>Background/Aim
Biotin is a vital micronutrient that plays a role in metabolic homeostasis and the regulation of innate and adaptive immune system functions. Biotinidase deficiency (BTD) leads to impairment in biotin‐dependent immune functions. This study focused on immunophenotypic analysis of lymphocyte subsets in newborns with BTD.
Patients and Methods
A total of 181 (95 female and 86 male; 114 had BTD and 67 were healthy) newborns underwent biotinidase enzyme activity, molecular and lymphocyte immunophenotyping analyses. BTD is classified into four biochemical phenotypes: profound, partial, heterozygous and normal. The following lymphocyte subsets were studied in all participants: total B lymphocyte (CD19), total T lymphocyte (CD3), helper T lymphocyte (CD3/CD4), cytotoxic T lymphocyte (CTL) (CD3/CD8), natural killer T lymphocyte (CD16/56) and a T‐lymphocyte activation marker (HLA‐DR).
Results
The percentages of lymphocyte subsets were similar in newborns with and without BTD. In all newborns with BTD, the mean CD3/CD4 levels were higher in females, while the CD3/CD8 levels were higher in males (P < .001 for each). In female and male newborns, the CD3/CD4 levels were 53.83 ± 9.46 and 16.82 ± 5.19, respectively, and the CD3/CD8 levels were 48.80 ± 8.65 and 21.48 ± 6.02, respectively. A moderate negative correlation was found between CD3/CD4 and CD3/CD8 in female and male newborns (rfemale = −0.488, rmale = −0.574, P < .001).
Conclusion
This study showed that although there were no differences in the lymphocyte subsets in newborns with BTD, the CD3/CD4 levels were higher in females, and the CD3/CD8 levels were higher in males. In addition, there was a negative correlation between the CD3/CD4 and CD3/CD8 levels in both genders. Although these results indicate sexual dimorphism between CD3/CD4 and CD3/CD8 levels, whether this dissociation is unique to BTD in newborns is not fully clear.</description><identifier>ISSN: 0905-6157</identifier><identifier>EISSN: 1399-3038</identifier><identifier>DOI: 10.1111/pai.13416</identifier><identifier>PMID: 33217065</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Biotin ; Biotinidase ; biotinidase deficiency ; CD16 antigen ; CD19 antigen ; CD3 antigen ; CD4 antigen ; CD8 antigen ; Cell activation ; Cytotoxicity ; Enzymatic activity ; Females ; Histocompatibility antigen HLA ; Homeostasis ; Immune system ; lymphocyte immunophenotyping ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Males ; Neonates ; Phenotypes ; Sexual dimorphism ; sexual immune dimorphism</subject><ispartof>Pediatric allergy and immunology, 2021-04, Vol.32 (3), p.586-598</ispartof><rights>2020 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3136-b2c10693650c2040795252f7cda5e144a22aa8f8a112f26f9ee2c157dcaf49d13</cites><orcidid>0000-0002-8512-8833 ; 0000-0002-2861-3568 ; 0000-0002-5544-8991 ; 0000-0002-3061-7832</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpai.13416$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpai.13416$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33217065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Atanaskovic‐Markovic, Marina</contributor><creatorcontrib>Karaoglan, Murat</creatorcontrib><creatorcontrib>Nacarkahya, Gulper</creatorcontrib><creatorcontrib>Keskin, Mehmet</creatorcontrib><creatorcontrib>Keskin, Ozlem</creatorcontrib><creatorcontrib>Atanaskovic‐Markovic, Marina</creatorcontrib><title>Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency</title><title>Pediatric allergy and immunology</title><addtitle>Pediatr Allergy Immunol</addtitle><description>Background/Aim
Biotin is a vital micronutrient that plays a role in metabolic homeostasis and the regulation of innate and adaptive immune system functions. Biotinidase deficiency (BTD) leads to impairment in biotin‐dependent immune functions. This study focused on immunophenotypic analysis of lymphocyte subsets in newborns with BTD.
Patients and Methods
A total of 181 (95 female and 86 male; 114 had BTD and 67 were healthy) newborns underwent biotinidase enzyme activity, molecular and lymphocyte immunophenotyping analyses. BTD is classified into four biochemical phenotypes: profound, partial, heterozygous and normal. The following lymphocyte subsets were studied in all participants: total B lymphocyte (CD19), total T lymphocyte (CD3), helper T lymphocyte (CD3/CD4), cytotoxic T lymphocyte (CTL) (CD3/CD8), natural killer T lymphocyte (CD16/56) and a T‐lymphocyte activation marker (HLA‐DR).
Results
The percentages of lymphocyte subsets were similar in newborns with and without BTD. In all newborns with BTD, the mean CD3/CD4 levels were higher in females, while the CD3/CD8 levels were higher in males (P < .001 for each). In female and male newborns, the CD3/CD4 levels were 53.83 ± 9.46 and 16.82 ± 5.19, respectively, and the CD3/CD8 levels were 48.80 ± 8.65 and 21.48 ± 6.02, respectively. A moderate negative correlation was found between CD3/CD4 and CD3/CD8 in female and male newborns (rfemale = −0.488, rmale = −0.574, P < .001).
Conclusion
This study showed that although there were no differences in the lymphocyte subsets in newborns with BTD, the CD3/CD4 levels were higher in females, and the CD3/CD8 levels were higher in males. In addition, there was a negative correlation between the CD3/CD4 and CD3/CD8 levels in both genders. Although these results indicate sexual dimorphism between CD3/CD4 and CD3/CD8 levels, whether this dissociation is unique to BTD in newborns is not fully clear.</description><subject>Biotin</subject><subject>Biotinidase</subject><subject>biotinidase deficiency</subject><subject>CD16 antigen</subject><subject>CD19 antigen</subject><subject>CD3 antigen</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell activation</subject><subject>Cytotoxicity</subject><subject>Enzymatic activity</subject><subject>Females</subject><subject>Histocompatibility antigen HLA</subject><subject>Homeostasis</subject><subject>Immune system</subject><subject>lymphocyte immunophenotyping</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Males</subject><subject>Neonates</subject><subject>Phenotypes</subject><subject>Sexual dimorphism</subject><subject>sexual immune dimorphism</subject><issn>0905-6157</issn><issn>1399-3038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10MFO3DAQBmALgcoWeugLIEtc2kPAY8fO5ohWtF1pJThQcYwcZ6w1SuwQJ1rl7TEN9IDEXObyzS_NT8h3YFeQ5rrX7gpEDuqIrECUZSaYWB-TFSuZzBTI4pR8jfGJMSiEgi_kVAgOBVNyRR63XTf50O_Rh3HunaHa63aOLtJgaTt3_T6YeUQapzriGKnz1OOhDoOP9ODGPa1dGJ13jY5IG7TOOPRmPicnVrcRv73tM_L31-3D5k-2u_u93dzsMiNAqKzmBpgqhZLMcJazopRccluYRkuEPNeca722aw3ALVe2REwXsmiMtnnZgDgjP5bcfgjPE8ax6lw02LbaY5hixXMlAFgu14lefqBPYRrSt0lJVnKe80Im9XNRZggxDmirfnCdHuYKWPXadpXarv61nezFW-JUd9j8l-_1JnC9gINrcf48qbq_2S6RL3efiSE</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Karaoglan, Murat</creator><creator>Nacarkahya, Gulper</creator><creator>Keskin, Mehmet</creator><creator>Keskin, Ozlem</creator><creator>Atanaskovic‐Markovic, Marina</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8512-8833</orcidid><orcidid>https://orcid.org/0000-0002-2861-3568</orcidid><orcidid>https://orcid.org/0000-0002-5544-8991</orcidid><orcidid>https://orcid.org/0000-0002-3061-7832</orcidid></search><sort><creationdate>202104</creationdate><title>Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency</title><author>Karaoglan, Murat ; Nacarkahya, Gulper ; Keskin, Mehmet ; Keskin, Ozlem ; Atanaskovic‐Markovic, Marina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3136-b2c10693650c2040795252f7cda5e144a22aa8f8a112f26f9ee2c157dcaf49d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biotin</topic><topic>Biotinidase</topic><topic>biotinidase deficiency</topic><topic>CD16 antigen</topic><topic>CD19 antigen</topic><topic>CD3 antigen</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell activation</topic><topic>Cytotoxicity</topic><topic>Enzymatic activity</topic><topic>Females</topic><topic>Histocompatibility antigen HLA</topic><topic>Homeostasis</topic><topic>Immune system</topic><topic>lymphocyte immunophenotyping</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Males</topic><topic>Neonates</topic><topic>Phenotypes</topic><topic>Sexual dimorphism</topic><topic>sexual immune dimorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karaoglan, Murat</creatorcontrib><creatorcontrib>Nacarkahya, Gulper</creatorcontrib><creatorcontrib>Keskin, Mehmet</creatorcontrib><creatorcontrib>Keskin, Ozlem</creatorcontrib><creatorcontrib>Atanaskovic‐Markovic, Marina</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karaoglan, Murat</au><au>Nacarkahya, Gulper</au><au>Keskin, Mehmet</au><au>Keskin, Ozlem</au><au>Atanaskovic‐Markovic, Marina</au><au>Atanaskovic‐Markovic, Marina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency</atitle><jtitle>Pediatric allergy and immunology</jtitle><addtitle>Pediatr Allergy Immunol</addtitle><date>2021-04</date><risdate>2021</risdate><volume>32</volume><issue>3</issue><spage>586</spage><epage>598</epage><pages>586-598</pages><issn>0905-6157</issn><eissn>1399-3038</eissn><abstract>Background/Aim
Biotin is a vital micronutrient that plays a role in metabolic homeostasis and the regulation of innate and adaptive immune system functions. Biotinidase deficiency (BTD) leads to impairment in biotin‐dependent immune functions. This study focused on immunophenotypic analysis of lymphocyte subsets in newborns with BTD.
Patients and Methods
A total of 181 (95 female and 86 male; 114 had BTD and 67 were healthy) newborns underwent biotinidase enzyme activity, molecular and lymphocyte immunophenotyping analyses. BTD is classified into four biochemical phenotypes: profound, partial, heterozygous and normal. The following lymphocyte subsets were studied in all participants: total B lymphocyte (CD19), total T lymphocyte (CD3), helper T lymphocyte (CD3/CD4), cytotoxic T lymphocyte (CTL) (CD3/CD8), natural killer T lymphocyte (CD16/56) and a T‐lymphocyte activation marker (HLA‐DR).
Results
The percentages of lymphocyte subsets were similar in newborns with and without BTD. In all newborns with BTD, the mean CD3/CD4 levels were higher in females, while the CD3/CD8 levels were higher in males (P < .001 for each). In female and male newborns, the CD3/CD4 levels were 53.83 ± 9.46 and 16.82 ± 5.19, respectively, and the CD3/CD8 levels were 48.80 ± 8.65 and 21.48 ± 6.02, respectively. A moderate negative correlation was found between CD3/CD4 and CD3/CD8 in female and male newborns (rfemale = −0.488, rmale = −0.574, P < .001).
Conclusion
This study showed that although there were no differences in the lymphocyte subsets in newborns with BTD, the CD3/CD4 levels were higher in females, and the CD3/CD8 levels were higher in males. In addition, there was a negative correlation between the CD3/CD4 and CD3/CD8 levels in both genders. Although these results indicate sexual dimorphism between CD3/CD4 and CD3/CD8 levels, whether this dissociation is unique to BTD in newborns is not fully clear.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33217065</pmid><doi>10.1111/pai.13416</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8512-8833</orcidid><orcidid>https://orcid.org/0000-0002-2861-3568</orcidid><orcidid>https://orcid.org/0000-0002-5544-8991</orcidid><orcidid>https://orcid.org/0000-0002-3061-7832</orcidid></addata></record> |
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| subjects | Biotin Biotinidase biotinidase deficiency CD16 antigen CD19 antigen CD3 antigen CD4 antigen CD8 antigen Cell activation Cytotoxicity Enzymatic activity Females Histocompatibility antigen HLA Homeostasis Immune system lymphocyte immunophenotyping Lymphocytes Lymphocytes B Lymphocytes T Males Neonates Phenotypes Sexual dimorphism sexual immune dimorphism |
| title | Immunophenotypic analysis of lymphocyte subsets in newborns with biotinidase deficiency |
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