Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy

Certain morphological parameters of the skeletal muscle tissue can be better understood via 3D considerations. Fluorescent confocal microscopy of thick tissue sections is a well‐established method for visualising and measuring skeletal muscle fibres and surrounding capillaries in 3D. However, thick...

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Veröffentlicht in:	Journal of microscopy (Oxford) 2021-05, Vol.282 (2), p.113-122
Hauptverfasser:	Umek, Nejc, Janáček, Jiří, Cvetko, Erika, Eržen, Ida
Format:	Artikel
Sprache:	eng
Schlagworte:	Calibration Capillaries Collapse Confocal microscopy Deformation Diameters Dilation Dimensionless numbers fibre diameter Fluorescence horizontal dilation Horizontal orientation Microscopy Microscopy, Confocal Microscopy, Fluorescence Microvasculature Muscle Fibers, Skeletal Muscle, Skeletal Muscles Musculoskeletal system Parameters sample deformation Sectioning Shrinkage shrinkage in Z direction Skeletal muscle
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