Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe
The stm1+ (SPAC17C9.10) gene of Schizosaccharomyces pombe is closely related to genes encoding vacuolar PQ-loop proteins, Ypq1, Ypq2, and Ypq3, of Saccharomyces cerevisiae. When stm1+ fused with GFP was expressed in fission or budding yeast, Stm1-GFP localized at the vacuolar membrane. Isolated vacu...
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| Veröffentlicht in: | Biochimica et biophysica acta. Biomembranes 2021-02, Vol.1863 (2), p.183507-183507, Article 183507 |
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| container_title | Biochimica et biophysica acta. Biomembranes |
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| creator | Kawano-Kawada, Miyuki Ueda, Taisuke Mori, Hikari Ichimura, Haruka Takegawa, Kaoru Sekito, Takayuki |
| description | The stm1+ (SPAC17C9.10) gene of Schizosaccharomyces pombe is closely related to genes encoding vacuolar PQ-loop proteins, Ypq1, Ypq2, and Ypq3, of Saccharomyces cerevisiae. When stm1+ fused with GFP was expressed in fission or budding yeast, Stm1-GFP localized at the vacuolar membrane. Isolated vacuolar membrane vesicles from S. cerevisiae cells overexpressing stm1+ exhibited stm1+-dependent arginine and lysine uptake activity. Exchange activity of arginine and histidine/arginine, as observed for Ypq2 of S. cerevisiae, was also detected in the vesicles expressing stm1+. The expression levels of stm1+ in S. pombe cells significantly affected the vacuolar contents of lysine, histidine, and arginine. These results suggest that Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids across the vacuolar membrane.
Transport of amino acids across the vacuolar membrane of fission yeast, Schizosaccharomyces pombe. [Display omitted]
•Stm1 of the fission yeast Schizosaccharomyces pombe is a vacuolar PQ-loop protein.•Transport activities of basic amino acids were detected with isolated vacuolar membrane vesicles expressing stm1+.•Stm1 is required for the amino acid compartmentalization in vacuoles of fission yeast. |
| doi_str_mv | 10.1016/j.bbamem.2020.183507 |
| format | Article |
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Transport of amino acids across the vacuolar membrane of fission yeast, Schizosaccharomyces pombe. [Display omitted]
•Stm1 of the fission yeast Schizosaccharomyces pombe is a vacuolar PQ-loop protein.•Transport activities of basic amino acids were detected with isolated vacuolar membrane vesicles expressing stm1+.•Stm1 is required for the amino acid compartmentalization in vacuoles of fission yeast.</description><identifier>ISSN: 0005-2736</identifier><identifier>EISSN: 1879-2642</identifier><identifier>DOI: 10.1016/j.bbamem.2020.183507</identifier><identifier>PMID: 33189720</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Arginine - genetics ; Arginine - metabolism ; Basic amino acids ; Biological Transport, Active - physiology ; Genetic Complementation Test ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Intracellular Membranes - metabolism ; Lysine - genetics ; Lysine - metabolism ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; PQ-loop ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Schizosaccharomyces - genetics ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Transport ; Vacuolar membrane protein ; Vacuoles - genetics ; Vacuoles - metabolism</subject><ispartof>Biochimica et biophysica acta. Biomembranes, 2021-02, Vol.1863 (2), p.183507-183507, Article 183507</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-83d6e90ac5223fb27bdf106b23f216e247bbd08eb92ff254cdded2d5ace65d2b3</citedby><cites>FETCH-LOGICAL-c362t-83d6e90ac5223fb27bdf106b23f216e247bbd08eb92ff254cdded2d5ace65d2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0005273620303503$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33189720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawano-Kawada, Miyuki</creatorcontrib><creatorcontrib>Ueda, Taisuke</creatorcontrib><creatorcontrib>Mori, Hikari</creatorcontrib><creatorcontrib>Ichimura, Haruka</creatorcontrib><creatorcontrib>Takegawa, Kaoru</creatorcontrib><creatorcontrib>Sekito, Takayuki</creatorcontrib><title>Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe</title><title>Biochimica et biophysica acta. Biomembranes</title><addtitle>Biochim Biophys Acta Biomembr</addtitle><description>The stm1+ (SPAC17C9.10) gene of Schizosaccharomyces pombe is closely related to genes encoding vacuolar PQ-loop proteins, Ypq1, Ypq2, and Ypq3, of Saccharomyces cerevisiae. When stm1+ fused with GFP was expressed in fission or budding yeast, Stm1-GFP localized at the vacuolar membrane. Isolated vacuolar membrane vesicles from S. cerevisiae cells overexpressing stm1+ exhibited stm1+-dependent arginine and lysine uptake activity. Exchange activity of arginine and histidine/arginine, as observed for Ypq2 of S. cerevisiae, was also detected in the vesicles expressing stm1+. The expression levels of stm1+ in S. pombe cells significantly affected the vacuolar contents of lysine, histidine, and arginine. These results suggest that Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids across the vacuolar membrane.
Transport of amino acids across the vacuolar membrane of fission yeast, Schizosaccharomyces pombe. [Display omitted]
•Stm1 of the fission yeast Schizosaccharomyces pombe is a vacuolar PQ-loop protein.•Transport activities of basic amino acids were detected with isolated vacuolar membrane vesicles expressing stm1+.•Stm1 is required for the amino acid compartmentalization in vacuoles of fission yeast.</description><subject>Arginine - genetics</subject><subject>Arginine - metabolism</subject><subject>Basic amino acids</subject><subject>Biological Transport, Active - physiology</subject><subject>Genetic Complementation Test</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Intracellular Membranes - metabolism</subject><subject>Lysine - genetics</subject><subject>Lysine - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>PQ-loop</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe Proteins - genetics</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Transport</subject><subject>Vacuolar membrane protein</subject><subject>Vacuoles - genetics</subject><subject>Vacuoles - metabolism</subject><issn>0005-2736</issn><issn>1879-2642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpaDbb_oNSdOzFG2lsy_alUEKaBAJJ2PQs9DFmtViWK3kXkl9fLU57zEmj4XlnmIeQr5xtOOPicr_RWnn0G2CQW21Zs-YDWfG26QoQFXwkK8ZYXUBTinNykdKe5VgF9SdyXpa87RpgKzJuZ8-pS1TRozKHMKhIH5-KIYSJTjHM6EbqxmMYjmhzQecd0jmqMU0hzjT0VKvkDFXejYEq42w6UVuzc68hKWN2Kgb_YjDRKXiNn8lZr4aEX97eNfn96_r56ra4f7i5u_p5X5hSwFy0pRXYMWVqgLLX0GjbcyZ0_gAXCFWjtWUt6g76HurKWIsWbK0MitqCLtfk-zI33_DngGmW3iWDw6BGDIckoRKs6zrRsYxWC2piSCliL6fovIovkjN5Mi33cjEtT6blYjrHvr1tOGiP9n_on9oM_FgAzHceHUaZjMPRoHURzSxtcO9v-As-vJJ6</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Kawano-Kawada, Miyuki</creator><creator>Ueda, Taisuke</creator><creator>Mori, Hikari</creator><creator>Ichimura, Haruka</creator><creator>Takegawa, Kaoru</creator><creator>Sekito, Takayuki</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210201</creationdate><title>Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe</title><author>Kawano-Kawada, Miyuki ; Ueda, Taisuke ; Mori, Hikari ; Ichimura, Haruka ; Takegawa, Kaoru ; Sekito, Takayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-83d6e90ac5223fb27bdf106b23f216e247bbd08eb92ff254cdded2d5ace65d2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Arginine - genetics</topic><topic>Arginine - metabolism</topic><topic>Basic amino acids</topic><topic>Biological Transport, Active - physiology</topic><topic>Genetic Complementation Test</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Intracellular Membranes - metabolism</topic><topic>Lysine - genetics</topic><topic>Lysine - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>PQ-loop</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe Proteins - genetics</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Transport</topic><topic>Vacuolar membrane protein</topic><topic>Vacuoles - genetics</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawano-Kawada, Miyuki</creatorcontrib><creatorcontrib>Ueda, Taisuke</creatorcontrib><creatorcontrib>Mori, Hikari</creatorcontrib><creatorcontrib>Ichimura, Haruka</creatorcontrib><creatorcontrib>Takegawa, Kaoru</creatorcontrib><creatorcontrib>Sekito, Takayuki</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimica et biophysica acta. Biomembranes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawano-Kawada, Miyuki</au><au>Ueda, Taisuke</au><au>Mori, Hikari</au><au>Ichimura, Haruka</au><au>Takegawa, Kaoru</au><au>Sekito, Takayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe</atitle><jtitle>Biochimica et biophysica acta. Biomembranes</jtitle><addtitle>Biochim Biophys Acta Biomembr</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>1863</volume><issue>2</issue><spage>183507</spage><epage>183507</epage><pages>183507-183507</pages><artnum>183507</artnum><issn>0005-2736</issn><eissn>1879-2642</eissn><abstract>The stm1+ (SPAC17C9.10) gene of Schizosaccharomyces pombe is closely related to genes encoding vacuolar PQ-loop proteins, Ypq1, Ypq2, and Ypq3, of Saccharomyces cerevisiae. When stm1+ fused with GFP was expressed in fission or budding yeast, Stm1-GFP localized at the vacuolar membrane. Isolated vacuolar membrane vesicles from S. cerevisiae cells overexpressing stm1+ exhibited stm1+-dependent arginine and lysine uptake activity. Exchange activity of arginine and histidine/arginine, as observed for Ypq2 of S. cerevisiae, was also detected in the vesicles expressing stm1+. The expression levels of stm1+ in S. pombe cells significantly affected the vacuolar contents of lysine, histidine, and arginine. These results suggest that Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids across the vacuolar membrane.
Transport of amino acids across the vacuolar membrane of fission yeast, Schizosaccharomyces pombe. [Display omitted]
•Stm1 of the fission yeast Schizosaccharomyces pombe is a vacuolar PQ-loop protein.•Transport activities of basic amino acids were detected with isolated vacuolar membrane vesicles expressing stm1+.•Stm1 is required for the amino acid compartmentalization in vacuoles of fission yeast.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33189720</pmid><doi>10.1016/j.bbamem.2020.183507</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Arginine - genetics Arginine - metabolism Basic amino acids Biological Transport, Active - physiology Genetic Complementation Test Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Intracellular Membranes - metabolism Lysine - genetics Lysine - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism PQ-loop Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Schizosaccharomyces - genetics Schizosaccharomyces - metabolism Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Transport Vacuolar membrane protein Vacuoles - genetics Vacuoles - metabolism |
| title | Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe |
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