TaMYB86B encodes a R2R3-type MYB transcription factor and enhances salt tolerance in wheat

•TaMYB86B is a R2R3-type MYB family gene located in the nucleus and has transcriptional activity in wheat.•The expression of TaMYB86B is induced by NaCl, ABA, GA, IAA, MeJA, and low temperature treatments.•Overexpression of TaMYB86B can increase the salt resistance of wheat by regulating the ion homeostasis to maintain an appropriate osmotic balance and decrease ROS levels. The MYB transcription factor family is important for plant responses to abiotic stresses. In this study, we identified three wheat TaMYB86 genes encoding R2R3-type MYB transcription factors. Analyses of the phylogenetic relationships and gene structures of TaMYB86A, TaMYB86B, and TaMYB86D revealed considerable similarities in gene structures and the encoded amino acid sequences. Additionally, TaMYB86B was highly expressed in the roots, stems, and leaves, suggesting it is critical for regulating salt stress responses in wheat. Moreover, TaMYB86B expression was induced by NaCl, abscisic acid (ABA), methyl jasmonate (MeJA), gibberellin (GA), auxin and low temperature treatments. The TaMYB86B protein localized in the nucleus and exhibited transcriptional activation activity. Under salt stress, TaMYB86B-overexpressing plants had a higher biomass and potassium ion (K+) content, but lower MDA, H2O2, O2−., and sodium ion (Na+) contents, when compared with the wild-type plants. Quantitative real-time PCR results indicated that the overexpression of TaMYB86B improved the expression of many stress-related genes. These findings suggest that TaMYB86B influences the salt tolerance of wheat by regulating the ion homeostasis to maintain an appropriate osmotic balance and decrease ROS levels.