New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis
Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei (L....
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| Veröffentlicht in: | Marine pollution bulletin 2020-11, Vol.160, p.111673-111673, Article 111673 |
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| creator | Jiao, Lefei Dai, Tianmeng Cao, Tinglan Jin, Min Sun, Peng Zhou, Qicun |
| description | Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei (L. vannamei), understanding the impact of marine Cr pollution is deemed to be significant. In this study, we used the transcriptome sequencing (RNA-seq) technique to characterize the molecular mechanism of Cr exposure in L. vannamei. Gene ontology enrichment analysis showed substrate-specific and ion transport-related functions were mainly influenced by Cr exposure. We further identified genes involved in protein digestion and absorption (PEPT1, BAT1, MDU1), chemical carcinogenesis (GST and UGTs), ABC transporters (ABCC2), apoptosis (CAPN1, CASP10, PARP), implying the potentially Cr disintoxication mechanisms in L. vannamei. Genes within pancreatic secretion (ALT, LDH), lysosome (CTSL and HEXB), and peroxisome (ACOX1, ECI2, NUDT12) pathways implied the potentially Cr toxicity mechanisms in L. vannamei.
•Cr exposure influenced hepatopancreas morphology in L. vannamei, which was characterized by increased B cells.•Substrate-specific and ion transport related function were most enriched GO terms after Cr exposure in L.vannamei.•Key genes that involved in Cr toxicity and disintoxication were identified in L.vannamei. |
| doi_str_mv | 10.1016/j.marpolbul.2020.111673 |
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•Cr exposure influenced hepatopancreas morphology in L. vannamei, which was characterized by increased B cells.•Substrate-specific and ion transport related function were most enriched GO terms after Cr exposure in L.vannamei.•Key genes that involved in Cr toxicity and disintoxication were identified in L.vannamei.</description><identifier>ISSN: 0025-326X</identifier><identifier>EISSN: 1879-3363</identifier><identifier>DOI: 10.1016/j.marpolbul.2020.111673</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Agricultural pollution ; Apoptosis ; Aquatic environment ; Carcinogenesis ; Carcinogens ; Chromium ; Chromium exposure ; Crustaceans ; Economic importance ; Economics ; Exposure ; Gene expression ; Gene sequencing ; Genes ; Heavy metals ; Industrial areas ; Industrial pollution ; Ion transport ; Ions ; Litopenaeus vannamei ; Lysosomes ; Marine pollution ; Nucleic acids ; Pancreas ; Ribonucleic acid ; RNA ; Sea pollution ; Secretion ; Shellfish ; Substrates ; Toxicity ; Transcriptome analysis ; Transcriptomes</subject><ispartof>Marine pollution bulletin, 2020-11, Vol.160, p.111673-111673, Article 111673</ispartof><rights>2020</rights><rights>Copyright Elsevier BV Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-748fd3bb33b06b8b1a56221e710ddc4f4e66099aff1c42273963401f719ff613</citedby><cites>FETCH-LOGICAL-c376t-748fd3bb33b06b8b1a56221e710ddc4f4e66099aff1c42273963401f719ff613</cites><orcidid>0000-0003-0665-9730</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.marpolbul.2020.111673$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Jiao, Lefei</creatorcontrib><creatorcontrib>Dai, Tianmeng</creatorcontrib><creatorcontrib>Cao, Tinglan</creatorcontrib><creatorcontrib>Jin, Min</creatorcontrib><creatorcontrib>Sun, Peng</creatorcontrib><creatorcontrib>Zhou, Qicun</creatorcontrib><title>New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis</title><title>Marine pollution bulletin</title><description>Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei (L. vannamei), understanding the impact of marine Cr pollution is deemed to be significant. In this study, we used the transcriptome sequencing (RNA-seq) technique to characterize the molecular mechanism of Cr exposure in L. vannamei. Gene ontology enrichment analysis showed substrate-specific and ion transport-related functions were mainly influenced by Cr exposure. We further identified genes involved in protein digestion and absorption (PEPT1, BAT1, MDU1), chemical carcinogenesis (GST and UGTs), ABC transporters (ABCC2), apoptosis (CAPN1, CASP10, PARP), implying the potentially Cr disintoxication mechanisms in L. vannamei. Genes within pancreatic secretion (ALT, LDH), lysosome (CTSL and HEXB), and peroxisome (ACOX1, ECI2, NUDT12) pathways implied the potentially Cr toxicity mechanisms in L. vannamei.
•Cr exposure influenced hepatopancreas morphology in L. vannamei, which was characterized by increased B cells.•Substrate-specific and ion transport related function were most enriched GO terms after Cr exposure in L.vannamei.•Key genes that involved in Cr toxicity and disintoxication were identified in L.vannamei.</description><subject>Agricultural pollution</subject><subject>Apoptosis</subject><subject>Aquatic environment</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Chromium</subject><subject>Chromium exposure</subject><subject>Crustaceans</subject><subject>Economic importance</subject><subject>Economics</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Heavy metals</subject><subject>Industrial areas</subject><subject>Industrial pollution</subject><subject>Ion transport</subject><subject>Ions</subject><subject>Litopenaeus vannamei</subject><subject>Lysosomes</subject><subject>Marine pollution</subject><subject>Nucleic acids</subject><subject>Pancreas</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sea pollution</subject><subject>Secretion</subject><subject>Shellfish</subject><subject>Substrates</subject><subject>Toxicity</subject><subject>Transcriptome analysis</subject><subject>Transcriptomes</subject><issn>0025-326X</issn><issn>1879-3363</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkUtP4zAUha0RI1GY-Q1YYsMmxY_UTpYV4jFSBZsu2FmOcz11J4mD7QD99zjqiAUbVlc6-s7RvfcgdEHJkhIqrvfLXofRd83ULRlhWaVUSP4DLWgl64JzwU_QghC2KjgTz6foLMY9IUQySRfo3yO8YTdE93eX8kwepx3g3ndgpk4H3OjoIvYWm13wvZt6DO-jj1OAWdy45EcYNEwRv-ph0D043BxwCnqIJrgx-R6wHnR3yDG_0E-ruwi__89ztL273d48FJun-z83601huBSpkGVlW940nDdENFVD9UowRkFS0ramtCUIQepaW0tNyZjkteAloVbS2lpB-Tm6OsaOwb9MEJPqXTTQdXoAP0XFSkFkTmAzevkF3fsp5HVnSlYVY5VgmZJHygQfYwCrxuDy0w-KEjV3oPbqswM1d6COHWTn-uiEfO6rg6CicTAYaF0Ak1Tr3bcZH3kPlV4</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Jiao, Lefei</creator><creator>Dai, Tianmeng</creator><creator>Cao, Tinglan</creator><creator>Jin, Min</creator><creator>Sun, Peng</creator><creator>Zhou, Qicun</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7TV</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0665-9730</orcidid></search><sort><creationdate>202011</creationdate><title>New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis</title><author>Jiao, Lefei ; Dai, Tianmeng ; Cao, Tinglan ; Jin, Min ; Sun, Peng ; Zhou, Qicun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-748fd3bb33b06b8b1a56221e710ddc4f4e66099aff1c42273963401f719ff613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agricultural pollution</topic><topic>Apoptosis</topic><topic>Aquatic environment</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Chromium</topic><topic>Chromium exposure</topic><topic>Crustaceans</topic><topic>Economic importance</topic><topic>Economics</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Heavy metals</topic><topic>Industrial areas</topic><topic>Industrial pollution</topic><topic>Ion transport</topic><topic>Ions</topic><topic>Litopenaeus vannamei</topic><topic>Lysosomes</topic><topic>Marine pollution</topic><topic>Nucleic acids</topic><topic>Pancreas</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sea pollution</topic><topic>Secretion</topic><topic>Shellfish</topic><topic>Substrates</topic><topic>Toxicity</topic><topic>Transcriptome analysis</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiao, Lefei</creatorcontrib><creatorcontrib>Dai, Tianmeng</creatorcontrib><creatorcontrib>Cao, Tinglan</creatorcontrib><creatorcontrib>Jin, Min</creatorcontrib><creatorcontrib>Sun, Peng</creatorcontrib><creatorcontrib>Zhou, Qicun</creatorcontrib><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Marine pollution bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiao, Lefei</au><au>Dai, Tianmeng</au><au>Cao, Tinglan</au><au>Jin, Min</au><au>Sun, Peng</au><au>Zhou, Qicun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis</atitle><jtitle>Marine pollution bulletin</jtitle><date>2020-11</date><risdate>2020</risdate><volume>160</volume><spage>111673</spage><epage>111673</epage><pages>111673-111673</pages><artnum>111673</artnum><issn>0025-326X</issn><eissn>1879-3363</eissn><abstract>Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei (L. vannamei), understanding the impact of marine Cr pollution is deemed to be significant. In this study, we used the transcriptome sequencing (RNA-seq) technique to characterize the molecular mechanism of Cr exposure in L. vannamei. Gene ontology enrichment analysis showed substrate-specific and ion transport-related functions were mainly influenced by Cr exposure. We further identified genes involved in protein digestion and absorption (PEPT1, BAT1, MDU1), chemical carcinogenesis (GST and UGTs), ABC transporters (ABCC2), apoptosis (CAPN1, CASP10, PARP), implying the potentially Cr disintoxication mechanisms in L. vannamei. Genes within pancreatic secretion (ALT, LDH), lysosome (CTSL and HEXB), and peroxisome (ACOX1, ECI2, NUDT12) pathways implied the potentially Cr toxicity mechanisms in L. vannamei.
•Cr exposure influenced hepatopancreas morphology in L. vannamei, which was characterized by increased B cells.•Substrate-specific and ion transport related function were most enriched GO terms after Cr exposure in L.vannamei.•Key genes that involved in Cr toxicity and disintoxication were identified in L.vannamei.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.marpolbul.2020.111673</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0665-9730</orcidid></addata></record> |
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| subjects | Agricultural pollution Apoptosis Aquatic environment Carcinogenesis Carcinogens Chromium Chromium exposure Crustaceans Economic importance Economics Exposure Gene expression Gene sequencing Genes Heavy metals Industrial areas Industrial pollution Ion transport Ions Litopenaeus vannamei Lysosomes Marine pollution Nucleic acids Pancreas Ribonucleic acid RNA Sea pollution Secretion Shellfish Substrates Toxicity Transcriptome analysis Transcriptomes |
| title | New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis |
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