FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)
Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcr...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2020-11, Vol.68 (47), p.13685-13696 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Yao, Panfeng Huang, Yunji Dong, Qixin Wan, Min Wang, Anhu Chen, Yuwei Li, Chenglei Wu, Qi Chen, Hui Zhao, Haixia |
| description | Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed that FtMYB6 is located in the nucleus and acts as a transcriptional activator. The FtMYB6 promoter showed strong spatiotemporal specificity and was induced by light. The expression of FtMYB6 showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression of FtMYB6 in transgenic Tartary buckwheat hairy roots and tobacco (Nicotiana tabacum) plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity of FtF3H and FtFLS1 promoters and inhibited the activity of the Ft4CL promoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activating FtF3H and FtFLS1 expression. |
| doi_str_mv | 10.1021/acs.jafc.0c03037 |
| format | Article |
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However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed that FtMYB6 is located in the nucleus and acts as a transcriptional activator. The FtMYB6 promoter showed strong spatiotemporal specificity and was induced by light. The expression of FtMYB6 showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression of FtMYB6 in transgenic Tartary buckwheat hairy roots and tobacco (Nicotiana tabacum) plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity of FtF3H and FtFLS1 promoters and inhibited the activity of the Ft4CL promoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activating FtF3H and FtFLS1 expression.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.0c03037</identifier><identifier>PMID: 33171044</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bioactive Constituents, Metabolites, and Functions ; Fagopyrum - genetics ; Fagopyrum - metabolism ; Flavonoids ; Flavonols ; Gene Expression Regulation, Plant ; Humans ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2020-11, Vol.68 (47), p.13685-13696</ispartof><rights>2020 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-b8611a97d9f6580c1a873caa0f45a1543efc9c7a7b39affa975b573cb04fdd183</citedby><cites>FETCH-LOGICAL-a336t-b8611a97d9f6580c1a873caa0f45a1543efc9c7a7b39affa975b573cb04fdd183</cites><orcidid>0000-0001-7864-906X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.0c03037$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.0c03037$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27078,27926,27927,56740,56790</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33171044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Panfeng</creatorcontrib><creatorcontrib>Huang, Yunji</creatorcontrib><creatorcontrib>Dong, Qixin</creatorcontrib><creatorcontrib>Wan, Min</creatorcontrib><creatorcontrib>Wang, Anhu</creatorcontrib><creatorcontrib>Chen, Yuwei</creatorcontrib><creatorcontrib>Li, Chenglei</creatorcontrib><creatorcontrib>Wu, Qi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Zhao, Haixia</creatorcontrib><title>FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed that FtMYB6 is located in the nucleus and acts as a transcriptional activator. The FtMYB6 promoter showed strong spatiotemporal specificity and was induced by light. The expression of FtMYB6 showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression of FtMYB6 in transgenic Tartary buckwheat hairy roots and tobacco (Nicotiana tabacum) plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity of FtF3H and FtFLS1 promoters and inhibited the activity of the Ft4CL promoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activating FtF3H and FtFLS1 expression.</description><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Fagopyrum - genetics</subject><subject>Fagopyrum - metabolism</subject><subject>Flavonoids</subject><subject>Flavonols</subject><subject>Gene Expression Regulation, Plant</subject><subject>Humans</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1v2zAURYmgReKk3TMFHFPAch9FUR9jHVRJABctUnfoJDxRZMxUEl2SauA9PzxM7Hbr9IZ77gXeIeScwYJByj6i9IsH1HIBEjjw4ojMmEghEYyVb8gMIpOUImcn5NT7BwAoRQHH5IRzVjDIshl5qsOXn8t8TpGuzP0mJLdjN0nV0e_XBb1L73gSY7p2OHrpzDYYO9IaZbBuTr85O9igPK17_GNH29OlsX43ho3yxlMz0jW6gG5Hl5P89bhRGOhljfd2u3PTQAPGzMhp-PCOvNXYe_X-cM_Ij_rz-uomWX29vr36tEqQ8zwkbZkzhlXRVToXJUiGZcElIuhMIBMZV1pWssCi5RVqHUnRiki0kOmuYyU_I5f73a2zvyflQzMYL1Xf46js5Js0E1WeirTkEYU9Kp313indbJ0Z4i8Ng-bFfRPdNy_um4P7WLk4rE_toLp_hb-yIzDfA69VO7kxPvv_vWc2ZpAD</recordid><startdate>20201125</startdate><enddate>20201125</enddate><creator>Yao, Panfeng</creator><creator>Huang, Yunji</creator><creator>Dong, Qixin</creator><creator>Wan, Min</creator><creator>Wang, Anhu</creator><creator>Chen, Yuwei</creator><creator>Li, Chenglei</creator><creator>Wu, Qi</creator><creator>Chen, Hui</creator><creator>Zhao, Haixia</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7864-906X</orcidid></search><sort><creationdate>20201125</creationdate><title>FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)</title><author>Yao, Panfeng ; Huang, Yunji ; Dong, Qixin ; Wan, Min ; Wang, Anhu ; Chen, Yuwei ; Li, Chenglei ; Wu, Qi ; Chen, Hui ; Zhao, Haixia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-b8611a97d9f6580c1a873caa0f45a1543efc9c7a7b39affa975b573cb04fdd183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Fagopyrum - genetics</topic><topic>Fagopyrum - metabolism</topic><topic>Flavonoids</topic><topic>Flavonols</topic><topic>Gene Expression Regulation, Plant</topic><topic>Humans</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yao, Panfeng</creatorcontrib><creatorcontrib>Huang, Yunji</creatorcontrib><creatorcontrib>Dong, Qixin</creatorcontrib><creatorcontrib>Wan, Min</creatorcontrib><creatorcontrib>Wang, Anhu</creatorcontrib><creatorcontrib>Chen, Yuwei</creatorcontrib><creatorcontrib>Li, Chenglei</creatorcontrib><creatorcontrib>Wu, Qi</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Zhao, Haixia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yao, Panfeng</au><au>Huang, Yunji</au><au>Dong, Qixin</au><au>Wan, Min</au><au>Wang, Anhu</au><au>Chen, Yuwei</au><au>Li, Chenglei</au><au>Wu, Qi</au><au>Chen, Hui</au><au>Zhao, Haixia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-11-25</date><risdate>2020</risdate><volume>68</volume><issue>47</issue><spage>13685</spage><epage>13696</epage><pages>13685-13696</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed that FtMYB6 is located in the nucleus and acts as a transcriptional activator. The FtMYB6 promoter showed strong spatiotemporal specificity and was induced by light. The expression of FtMYB6 showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression of FtMYB6 in transgenic Tartary buckwheat hairy roots and tobacco (Nicotiana tabacum) plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity of FtF3H and FtFLS1 promoters and inhibited the activity of the Ft4CL promoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activating FtF3H and FtFLS1 expression.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33171044</pmid><doi>10.1021/acs.jafc.0c03037</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7864-906X</orcidid></addata></record> |
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| subjects | Bioactive Constituents, Metabolites, and Functions Fagopyrum - genetics Fagopyrum - metabolism Flavonoids Flavonols Gene Expression Regulation, Plant Humans Plant Proteins - genetics Plant Proteins - metabolism Transcription Factors - genetics Transcription Factors - metabolism |
| title | FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum) |
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