Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women?
Problem The definition of chronic endometritis (CE) differs among studies, and currently, there is no accepted consensus. This study aimed to establish the minimum number of immunohistochemical analysis of CD138+ plasma cells to identify a clinically relevant CE. Method of study We performed a retro...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2021-05, Vol.85 (5), p.e13369-n/a |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 85 |
| creator | Li, Yuye Xu, Shiru Yu, Shuyi Huang, Chunyu Lin, Shenglai Chen, Wanru Mo, Meilan Lian, Ruochun Diao, Lianghui Ding, Lijun Zeng, Yong |
| description | Problem
The definition of chronic endometritis (CE) differs among studies, and currently, there is no accepted consensus. This study aimed to establish the minimum number of immunohistochemical analysis of CD138+ plasma cells to identify a clinically relevant CE.
Method of study
We performed a retrospective study on 716 infertile patients who never did CE analysis and respective antibiotic treatment before. Samples were obtained by endometrial scratching in the mid‐luteal phase before IVF‐ET treatment. The number and distribution of CD138+ cells were analyzed by immunohistochemistry. Thirty high‐power fields (HPF) were evaluated for each sample. Patients were classified in 2 main groups: (a) CD138low ( |
| doi_str_mv | 10.1111/aji.13369 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2458034740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2511917658</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-d649a314e9040aff7b2f50100ec7456e91e4223f05f2cc766f48c351124a81f43</originalsourceid><addsrcrecordid>eNp1kctKAzEUhoMo1tvCF5CAG0XG5jY3N1JatYqgC12HmCY1ZSapyQxlVr66qeMFBLNJOHz5TnJ-AA4xOsdxDcXCnGNKs3ID7OAMoQQVZb4Zz4hlSc5QMQC7ISwQinWab4MBpTglmNAd8D4xYm5dMAE6DeWrd9ZIqOzM1arxpjHhAk7dCtbCdnA8wbQ4g1JVVRhOH6-hsb-oqGBovKsFFFor2cClV3MrrOygaxsZoXUHY7XyjakUXMWKvdwHW1pUQR187Xvg-frqaTxN7h9ubsej-0TSlJbJLGOloJipEjEU_fkL0SnCCCmZszRTJVaMEKpRqomUeZZpVsSbGBMmCqwZ3QMnvXfp3VurQsNrE9YfEVa5NnDC0gJRFocV0eM_6MK13sbXcRKNJc6ztIjUaU9J70LwSvOlN7XwHceIr1PhMRX-mUpkj76M7UutZj_kdwwRGPbAKk6m-9_ER3e3vfIDoZmU6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2511917658</pqid></control><display><type>article</type><title>Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women?</title><source>Wiley Journals</source><creator>Li, Yuye ; Xu, Shiru ; Yu, Shuyi ; Huang, Chunyu ; Lin, Shenglai ; Chen, Wanru ; Mo, Meilan ; Lian, Ruochun ; Diao, Lianghui ; Ding, Lijun ; Zeng, Yong</creator><creatorcontrib>Li, Yuye ; Xu, Shiru ; Yu, Shuyi ; Huang, Chunyu ; Lin, Shenglai ; Chen, Wanru ; Mo, Meilan ; Lian, Ruochun ; Diao, Lianghui ; Ding, Lijun ; Zeng, Yong</creatorcontrib><description>Problem
The definition of chronic endometritis (CE) differs among studies, and currently, there is no accepted consensus. This study aimed to establish the minimum number of immunohistochemical analysis of CD138+ plasma cells to identify a clinically relevant CE.
Method of study
We performed a retrospective study on 716 infertile patients who never did CE analysis and respective antibiotic treatment before. Samples were obtained by endometrial scratching in the mid‐luteal phase before IVF‐ET treatment. The number and distribution of CD138+ cells were analyzed by immunohistochemistry. Thirty high‐power fields (HPF) were evaluated for each sample. Patients were classified in 2 main groups: (a) CD138low (<5 CD138+ cells in all HPFs), (b) CD138high (≥5 CD138+ cells in at least one HPF). Pregnancy outcome was compared among the groups.
Results
In the CD138high group, β‐hCG positive rate, clinical pregnancy rate and live birth rate were significantly decreased (P = .04, P = .01, P = .04, respectively). Also after adjusting for patient age, body mass index (BMI), and clinical characteristics, the β‐hCG positive rate (P = .05), clinical pregnancy rate (P = .01) and live birth rate (P = .02) were significantly lower in the CD138high than those in the CD138low group. Within the CD138low group, these parameters were not significantly different between patients without any plasma cells and patients with up to 4 plasma cells/HPF.
Conclusion
We conclude that immunohistochemical analysis of CD138+ cells is a reliable method to detect CE which can be identified by the presence of ≥5 plasma cells in at least one out of 30 HPF.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13369</identifier><identifier>PMID: 33152123</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Birth rate ; Body mass index ; CD138 ; chronic endometritis ; Endometritis ; Endometrium ; Immunohistochemistry ; infertile women ; Patients ; Plasma ; Plasma cells ; Pregnancy ; pregnancy outcome ; Stroma</subject><ispartof>American journal of reproductive immunology (1989), 2021-05, Vol.85 (5), p.e13369-n/a</ispartof><rights>2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-d649a314e9040aff7b2f50100ec7456e91e4223f05f2cc766f48c351124a81f43</citedby><cites>FETCH-LOGICAL-c3539-d649a314e9040aff7b2f50100ec7456e91e4223f05f2cc766f48c351124a81f43</cites><orcidid>0000-0002-1159-9261 ; 0000-0002-6264-283X ; 0000-0001-5857-8540</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13369$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13369$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33152123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuye</creatorcontrib><creatorcontrib>Xu, Shiru</creatorcontrib><creatorcontrib>Yu, Shuyi</creatorcontrib><creatorcontrib>Huang, Chunyu</creatorcontrib><creatorcontrib>Lin, Shenglai</creatorcontrib><creatorcontrib>Chen, Wanru</creatorcontrib><creatorcontrib>Mo, Meilan</creatorcontrib><creatorcontrib>Lian, Ruochun</creatorcontrib><creatorcontrib>Diao, Lianghui</creatorcontrib><creatorcontrib>Ding, Lijun</creatorcontrib><creatorcontrib>Zeng, Yong</creatorcontrib><title>Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women?</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
The definition of chronic endometritis (CE) differs among studies, and currently, there is no accepted consensus. This study aimed to establish the minimum number of immunohistochemical analysis of CD138+ plasma cells to identify a clinically relevant CE.
Method of study
We performed a retrospective study on 716 infertile patients who never did CE analysis and respective antibiotic treatment before. Samples were obtained by endometrial scratching in the mid‐luteal phase before IVF‐ET treatment. The number and distribution of CD138+ cells were analyzed by immunohistochemistry. Thirty high‐power fields (HPF) were evaluated for each sample. Patients were classified in 2 main groups: (a) CD138low (<5 CD138+ cells in all HPFs), (b) CD138high (≥5 CD138+ cells in at least one HPF). Pregnancy outcome was compared among the groups.
Results
In the CD138high group, β‐hCG positive rate, clinical pregnancy rate and live birth rate were significantly decreased (P = .04, P = .01, P = .04, respectively). Also after adjusting for patient age, body mass index (BMI), and clinical characteristics, the β‐hCG positive rate (P = .05), clinical pregnancy rate (P = .01) and live birth rate (P = .02) were significantly lower in the CD138high than those in the CD138low group. Within the CD138low group, these parameters were not significantly different between patients without any plasma cells and patients with up to 4 plasma cells/HPF.
Conclusion
We conclude that immunohistochemical analysis of CD138+ cells is a reliable method to detect CE which can be identified by the presence of ≥5 plasma cells in at least one out of 30 HPF.</description><subject>Birth rate</subject><subject>Body mass index</subject><subject>CD138</subject><subject>chronic endometritis</subject><subject>Endometritis</subject><subject>Endometrium</subject><subject>Immunohistochemistry</subject><subject>infertile women</subject><subject>Patients</subject><subject>Plasma</subject><subject>Plasma cells</subject><subject>Pregnancy</subject><subject>pregnancy outcome</subject><subject>Stroma</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kctKAzEUhoMo1tvCF5CAG0XG5jY3N1JatYqgC12HmCY1ZSapyQxlVr66qeMFBLNJOHz5TnJ-AA4xOsdxDcXCnGNKs3ID7OAMoQQVZb4Zz4hlSc5QMQC7ISwQinWab4MBpTglmNAd8D4xYm5dMAE6DeWrd9ZIqOzM1arxpjHhAk7dCtbCdnA8wbQ4g1JVVRhOH6-hsb-oqGBovKsFFFor2cClV3MrrOygaxsZoXUHY7XyjakUXMWKvdwHW1pUQR187Xvg-frqaTxN7h9ubsej-0TSlJbJLGOloJipEjEU_fkL0SnCCCmZszRTJVaMEKpRqomUeZZpVsSbGBMmCqwZ3QMnvXfp3VurQsNrE9YfEVa5NnDC0gJRFocV0eM_6MK13sbXcRKNJc6ztIjUaU9J70LwSvOlN7XwHceIr1PhMRX-mUpkj76M7UutZj_kdwwRGPbAKk6m-9_ER3e3vfIDoZmU6g</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Li, Yuye</creator><creator>Xu, Shiru</creator><creator>Yu, Shuyi</creator><creator>Huang, Chunyu</creator><creator>Lin, Shenglai</creator><creator>Chen, Wanru</creator><creator>Mo, Meilan</creator><creator>Lian, Ruochun</creator><creator>Diao, Lianghui</creator><creator>Ding, Lijun</creator><creator>Zeng, Yong</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1159-9261</orcidid><orcidid>https://orcid.org/0000-0002-6264-283X</orcidid><orcidid>https://orcid.org/0000-0001-5857-8540</orcidid></search><sort><creationdate>202105</creationdate><title>Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women?</title><author>Li, Yuye ; Xu, Shiru ; Yu, Shuyi ; Huang, Chunyu ; Lin, Shenglai ; Chen, Wanru ; Mo, Meilan ; Lian, Ruochun ; Diao, Lianghui ; Ding, Lijun ; Zeng, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-d649a314e9040aff7b2f50100ec7456e91e4223f05f2cc766f48c351124a81f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Birth rate</topic><topic>Body mass index</topic><topic>CD138</topic><topic>chronic endometritis</topic><topic>Endometritis</topic><topic>Endometrium</topic><topic>Immunohistochemistry</topic><topic>infertile women</topic><topic>Patients</topic><topic>Plasma</topic><topic>Plasma cells</topic><topic>Pregnancy</topic><topic>pregnancy outcome</topic><topic>Stroma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuye</creatorcontrib><creatorcontrib>Xu, Shiru</creatorcontrib><creatorcontrib>Yu, Shuyi</creatorcontrib><creatorcontrib>Huang, Chunyu</creatorcontrib><creatorcontrib>Lin, Shenglai</creatorcontrib><creatorcontrib>Chen, Wanru</creatorcontrib><creatorcontrib>Mo, Meilan</creatorcontrib><creatorcontrib>Lian, Ruochun</creatorcontrib><creatorcontrib>Diao, Lianghui</creatorcontrib><creatorcontrib>Ding, Lijun</creatorcontrib><creatorcontrib>Zeng, Yong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuye</au><au>Xu, Shiru</au><au>Yu, Shuyi</au><au>Huang, Chunyu</au><au>Lin, Shenglai</au><au>Chen, Wanru</au><au>Mo, Meilan</au><au>Lian, Ruochun</au><au>Diao, Lianghui</au><au>Ding, Lijun</au><au>Zeng, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women?</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2021-05</date><risdate>2021</risdate><volume>85</volume><issue>5</issue><spage>e13369</spage><epage>n/a</epage><pages>e13369-n/a</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
The definition of chronic endometritis (CE) differs among studies, and currently, there is no accepted consensus. This study aimed to establish the minimum number of immunohistochemical analysis of CD138+ plasma cells to identify a clinically relevant CE.
Method of study
We performed a retrospective study on 716 infertile patients who never did CE analysis and respective antibiotic treatment before. Samples were obtained by endometrial scratching in the mid‐luteal phase before IVF‐ET treatment. The number and distribution of CD138+ cells were analyzed by immunohistochemistry. Thirty high‐power fields (HPF) were evaluated for each sample. Patients were classified in 2 main groups: (a) CD138low (<5 CD138+ cells in all HPFs), (b) CD138high (≥5 CD138+ cells in at least one HPF). Pregnancy outcome was compared among the groups.
Results
In the CD138high group, β‐hCG positive rate, clinical pregnancy rate and live birth rate were significantly decreased (P = .04, P = .01, P = .04, respectively). Also after adjusting for patient age, body mass index (BMI), and clinical characteristics, the β‐hCG positive rate (P = .05), clinical pregnancy rate (P = .01) and live birth rate (P = .02) were significantly lower in the CD138high than those in the CD138low group. Within the CD138low group, these parameters were not significantly different between patients without any plasma cells and patients with up to 4 plasma cells/HPF.
Conclusion
We conclude that immunohistochemical analysis of CD138+ cells is a reliable method to detect CE which can be identified by the presence of ≥5 plasma cells in at least one out of 30 HPF.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33152123</pmid><doi>10.1111/aji.13369</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1159-9261</orcidid><orcidid>https://orcid.org/0000-0002-6264-283X</orcidid><orcidid>https://orcid.org/0000-0001-5857-8540</orcidid></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 2021-05, Vol.85 (5), p.e13369-n/a |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | Wiley Journals |
| subjects | Birth rate Body mass index CD138 chronic endometritis Endometritis Endometrium Immunohistochemistry infertile women Patients Plasma Plasma cells Pregnancy pregnancy outcome Stroma |
| title | Diagnosis of chronic endometritis: How many CD138+ cells/HPF in endometrial stroma affect pregnancy outcome of infertile women? |
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