Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov
A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000 T , was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (o...
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creator | Feng, Yu-Zhou Chunyu, Wei-Xun Liang, Rui Hahnke, Richard L. Schumann, Peter Zhao, Yu-Rong Gao, Sheng Yin, Min Ji, Yang Sun, Le Zhi, Xiao-Yang Tang, Shu-Kun |
description | A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000
T
, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3
γ
with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (> 10%) were C
16:0
, Summed In Feature 3 (C
16:1
ω6c
/C
16:1
ω7c
) and C
17:1
ω8c
. The predominant menaquinone was MK-9(H
4
). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000
T
with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000
T
together with the genus
Motilibacter
formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000
T
showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus
Motilibacter
. Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species
Vallicoccus soli
gen. nov., sp. nov. (YIM 75000
T
= DSM 45377
T
= KCTC 49228
T
= CGMCC 1.13844
T
) which is the nomenclature type of the novel genus
Vallicoccus
gen. nov. within
Vallicoccaceae
fam. nov and
Motilibacterales
ord. nov in the phylum Actinobacteria. The family
Vallicoccaceae
fam. nov. and the order
Motilibacterales
(contains
Vallicoccaceae
fam. nov. and
Motilibacteraceae
Lee
2013
) ord. nov. are formally proposed. |
doi_str_mv | 10.1007/s10482-020-01484-5 |
format | Article |
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T
, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3
γ
with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (> 10%) were C
16:0
, Summed In Feature 3 (C
16:1
ω6c
/C
16:1
ω7c
) and C
17:1
ω8c
. The predominant menaquinone was MK-9(H
4
). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000
T
with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000
T
together with the genus
Motilibacter
formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000
T
showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus
Motilibacter
. Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species
Vallicoccus soli
gen. nov., sp. nov. (YIM 75000
T
= DSM 45377
T
= KCTC 49228
T
= CGMCC 1.13844
T
) which is the nomenclature type of the novel genus
Vallicoccus
gen. nov. within
Vallicoccaceae
fam. nov and
Motilibacterales
ord. nov in the phylum Actinobacteria. The family
Vallicoccaceae
fam. nov. and the order
Motilibacterales
(contains
Vallicoccaceae
fam. nov. and
Motilibacteraceae
Lee
2013
) ord. nov. are formally proposed.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-020-01484-5</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Actinobacteria ; Alanine ; Arabinose ; Biomedical and Life Sciences ; Deoxyribonucleic acid ; DNA ; Fatty acids ; Gene sequencing ; Genomes ; Glutamic acid ; Glycine ; Gram stain ; Hybridization ; Life Sciences ; Mannose ; Medical Microbiology ; Menaquinones ; Microbiology ; New species ; Nucleotides ; Original Paper ; Peptidoglycans ; pH effects ; Phylogenetics ; Phylogeny ; Plant Sciences ; Rhamnose ; Ribose ; rRNA 16S ; Sodium chloride ; Soil Science & Conservation ; Sugar</subject><ispartof>Antonie van Leeuwenhoek, 2020-12, Vol.113 (12), p.2155-2165</ispartof><rights>Springer Nature Switzerland AG 2020</rights><rights>Springer Nature Switzerland AG 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-3d4233b14eea2dd59f25323c5551164661d6c33cd865f781561702bce1c413063</citedby><cites>FETCH-LOGICAL-c352t-3d4233b14eea2dd59f25323c5551164661d6c33cd865f781561702bce1c413063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-020-01484-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-020-01484-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Feng, Yu-Zhou</creatorcontrib><creatorcontrib>Chunyu, Wei-Xun</creatorcontrib><creatorcontrib>Liang, Rui</creatorcontrib><creatorcontrib>Hahnke, Richard L.</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Zhao, Yu-Rong</creatorcontrib><creatorcontrib>Gao, Sheng</creatorcontrib><creatorcontrib>Yin, Min</creatorcontrib><creatorcontrib>Ji, Yang</creatorcontrib><creatorcontrib>Sun, Le</creatorcontrib><creatorcontrib>Zhi, Xiao-Yang</creatorcontrib><creatorcontrib>Tang, Shu-Kun</creatorcontrib><title>Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><description>A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000
T
, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3
γ
with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (> 10%) were C
16:0
, Summed In Feature 3 (C
16:1
ω6c
/C
16:1
ω7c
) and C
17:1
ω8c
. The predominant menaquinone was MK-9(H
4
). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000
T
with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000
T
together with the genus
Motilibacter
formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000
T
showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus
Motilibacter
. Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species
Vallicoccus soli
gen. nov., sp. nov. (YIM 75000
T
= DSM 45377
T
= KCTC 49228
T
= CGMCC 1.13844
T
) which is the nomenclature type of the novel genus
Vallicoccus
gen. nov. within
Vallicoccaceae
fam. nov and
Motilibacterales
ord. nov in the phylum Actinobacteria. The family
Vallicoccaceae
fam. nov. and the order
Motilibacterales
(contains
Vallicoccaceae
fam. nov. and
Motilibacteraceae
Lee
2013
) ord. nov. are formally proposed.</description><subject>Actinobacteria</subject><subject>Alanine</subject><subject>Arabinose</subject><subject>Biomedical and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fatty acids</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Glutamic acid</subject><subject>Glycine</subject><subject>Gram stain</subject><subject>Hybridization</subject><subject>Life Sciences</subject><subject>Mannose</subject><subject>Medical Microbiology</subject><subject>Menaquinones</subject><subject>Microbiology</subject><subject>New species</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Peptidoglycans</subject><subject>pH effects</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Rhamnose</subject><subject>Ribose</subject><subject>rRNA 16S</subject><subject>Sodium chloride</subject><subject>Soil Science & Conservation</subject><subject>Sugar</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1O3DAUha2qSAzQF2BlqRsWzdT_TpYVKj8SqBvo1vLYNyOPnHhqJ0h9Ex4XQ6BILLo698rfOdfSQeiUkjUlRH8vlIiWNYSRhlDRikZ-QisqNWs61XWf0YoQwhtFNDtER6Xs6tqpVq_Q428bY3DJubngkmLAWxjXeEwP62-47N8m-6wQsXVTGNOmCuQwDzhUi53A4z6nofpDrOzosYficthPIY049fjfEevAAu7t8BZ8m6YQwxJoIxScsn95O0EHvY0FvrzqMbq_-Hl3ftXc_Lq8Pv9x0zgu2dRwLxjnGyoALPNedj2TnHEnpaRUCaWoV45z51sle91SqagmbOOAOkE5UfwYnS25-5z-zFAmM4TiIEY7QpqLYULqTpOWtRX9-gHdpTmP9XeV0pwLWslKsYVyOZWSoTf7HAab_xpKzHNZZinL1LLMS1lGVhNfTKXC4xbye_R_XE95RpZ4</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Feng, Yu-Zhou</creator><creator>Chunyu, Wei-Xun</creator><creator>Liang, Rui</creator><creator>Hahnke, Richard L.</creator><creator>Schumann, Peter</creator><creator>Zhao, Yu-Rong</creator><creator>Gao, Sheng</creator><creator>Yin, Min</creator><creator>Ji, Yang</creator><creator>Sun, Le</creator><creator>Zhi, Xiao-Yang</creator><creator>Tang, Shu-Kun</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20201201</creationdate><title>Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov</title><author>Feng, Yu-Zhou ; Chunyu, Wei-Xun ; Liang, Rui ; Hahnke, Richard L. ; Schumann, Peter ; Zhao, Yu-Rong ; Gao, Sheng ; Yin, Min ; Ji, Yang ; Sun, Le ; Zhi, Xiao-Yang ; Tang, Shu-Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-3d4233b14eea2dd59f25323c5551164661d6c33cd865f781561702bce1c413063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Actinobacteria</topic><topic>Alanine</topic><topic>Arabinose</topic><topic>Biomedical and Life Sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fatty acids</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Glutamic acid</topic><topic>Glycine</topic><topic>Gram stain</topic><topic>Hybridization</topic><topic>Life Sciences</topic><topic>Mannose</topic><topic>Medical Microbiology</topic><topic>Menaquinones</topic><topic>Microbiology</topic><topic>New species</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Peptidoglycans</topic><topic>pH effects</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Rhamnose</topic><topic>Ribose</topic><topic>rRNA 16S</topic><topic>Sodium chloride</topic><topic>Soil Science & Conservation</topic><topic>Sugar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Yu-Zhou</creatorcontrib><creatorcontrib>Chunyu, Wei-Xun</creatorcontrib><creatorcontrib>Liang, Rui</creatorcontrib><creatorcontrib>Hahnke, Richard L.</creatorcontrib><creatorcontrib>Schumann, Peter</creatorcontrib><creatorcontrib>Zhao, Yu-Rong</creatorcontrib><creatorcontrib>Gao, Sheng</creatorcontrib><creatorcontrib>Yin, Min</creatorcontrib><creatorcontrib>Ji, Yang</creatorcontrib><creatorcontrib>Sun, Le</creatorcontrib><creatorcontrib>Zhi, Xiao-Yang</creatorcontrib><creatorcontrib>Tang, Shu-Kun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni 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(Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Yu-Zhou</au><au>Chunyu, Wei-Xun</au><au>Liang, Rui</au><au>Hahnke, Richard L.</au><au>Schumann, Peter</au><au>Zhao, Yu-Rong</au><au>Gao, Sheng</au><au>Yin, Min</au><au>Ji, Yang</au><au>Sun, Le</au><au>Zhi, Xiao-Yang</au><au>Tang, Shu-Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><date>2020-12-01</date><risdate>2020</risdate><volume>113</volume><issue>12</issue><spage>2155</spage><epage>2165</epage><pages>2155-2165</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000
T
, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3
γ
with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (> 10%) were C
16:0
, Summed In Feature 3 (C
16:1
ω6c
/C
16:1
ω7c
) and C
17:1
ω8c
. The predominant menaquinone was MK-9(H
4
). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000
T
with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000
T
together with the genus
Motilibacter
formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000
T
showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus
Motilibacter
. Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species
Vallicoccus soli
gen. nov., sp. nov. (YIM 75000
T
= DSM 45377
T
= KCTC 49228
T
= CGMCC 1.13844
T
) which is the nomenclature type of the novel genus
Vallicoccus
gen. nov. within
Vallicoccaceae
fam. nov and
Motilibacterales
ord. nov in the phylum Actinobacteria. The family
Vallicoccaceae
fam. nov. and the order
Motilibacterales
(contains
Vallicoccaceae
fam. nov. and
Motilibacteraceae
Lee
2013
) ord. nov. are formally proposed.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s10482-020-01484-5</doi><tpages>11</tpages></addata></record> |
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source | SpringerNature Journals |
subjects | Actinobacteria Alanine Arabinose Biomedical and Life Sciences Deoxyribonucleic acid DNA Fatty acids Gene sequencing Genomes Glutamic acid Glycine Gram stain Hybridization Life Sciences Mannose Medical Microbiology Menaquinones Microbiology New species Nucleotides Original Paper Peptidoglycans pH effects Phylogenetics Phylogeny Plant Sciences Rhamnose Ribose rRNA 16S Sodium chloride Soil Science & Conservation Sugar |
title | Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov |
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