Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov

A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000 T , was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (o...

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Veröffentlicht in:Antonie van Leeuwenhoek 2020-12, Vol.113 (12), p.2155-2165
Hauptverfasser: Feng, Yu-Zhou, Chunyu, Wei-Xun, Liang, Rui, Hahnke, Richard L., Schumann, Peter, Zhao, Yu-Rong, Gao, Sheng, Yin, Min, Ji, Yang, Sun, Le, Zhi, Xiao-Yang, Tang, Shu-Kun
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container_title Antonie van Leeuwenhoek
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creator Feng, Yu-Zhou
Chunyu, Wei-Xun
Liang, Rui
Hahnke, Richard L.
Schumann, Peter
Zhao, Yu-Rong
Gao, Sheng
Yin, Min
Ji, Yang
Sun, Le
Zhi, Xiao-Yang
Tang, Shu-Kun
description A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000 T , was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3 γ with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (> 10%) were C 16:0 , Summed In Feature 3 (C 16:1 ω6c /C 16:1 ω7c ) and C 17:1 ω8c . The predominant menaquinone was MK-9(H 4 ). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000 T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000 T together with the genus Motilibacter formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000 T showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus Motilibacter . Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species Vallicoccus soli gen. nov., sp. nov. (YIM 75000 T  = DSM 45377 T  = KCTC 49228 T  = CGMCC 1.13844 T ) which is the nomenclature type of the novel genus Vallicoccus gen. nov. within Vallicoccaceae fam. nov and Motilibacterales ord. nov in the phylum Actinobacteria. The family Vallicoccaceae fam. nov. and the order Motilibacterales (contains Vallicoccaceae fam. nov. and Motilibacteraceae Lee 2013 ) ord. nov. are formally proposed.
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China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3 γ with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (&gt; 10%) were C 16:0 , Summed In Feature 3 (C 16:1 ω6c /C 16:1 ω7c ) and C 17:1 ω8c . The predominant menaquinone was MK-9(H 4 ). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000 T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000 T together with the genus Motilibacter formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. 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China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3 γ with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (&gt; 10%) were C 16:0 , Summed In Feature 3 (C 16:1 ω6c /C 16:1 ω7c ) and C 17:1 ω8c . The predominant menaquinone was MK-9(H 4 ). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000 T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000 T together with the genus Motilibacter formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000 T showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus Motilibacter . Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species Vallicoccus soli gen. nov., sp. nov. (YIM 75000 T  = DSM 45377 T  = KCTC 49228 T  = CGMCC 1.13844 T ) which is the nomenclature type of the novel genus Vallicoccus gen. nov. within Vallicoccaceae fam. nov and Motilibacterales ord. nov in the phylum Actinobacteria. The family Vallicoccaceae fam. nov. and the order Motilibacterales (contains Vallicoccaceae fam. nov. and Motilibacteraceae Lee 2013 ) ord. nov. are formally proposed.</description><subject>Actinobacteria</subject><subject>Alanine</subject><subject>Arabinose</subject><subject>Biomedical and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fatty acids</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Glutamic acid</subject><subject>Glycine</subject><subject>Gram stain</subject><subject>Hybridization</subject><subject>Life Sciences</subject><subject>Mannose</subject><subject>Medical Microbiology</subject><subject>Menaquinones</subject><subject>Microbiology</subject><subject>New species</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Peptidoglycans</subject><subject>pH effects</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Rhamnose</subject><subject>Ribose</subject><subject>rRNA 16S</subject><subject>Sodium chloride</subject><subject>Soil Science &amp; 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China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3 γ with an LL-Dpm-Gly interpeptide bridge. The major cellular fatty acids (&gt; 10%) were C 16:0 , Summed In Feature 3 (C 16:1 ω6c /C 16:1 ω7c ) and C 17:1 ω8c . The predominant menaquinone was MK-9(H 4 ). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G+C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000 T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000 T together with the genus Motilibacter formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000 T showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus Motilibacter . Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species Vallicoccus soli gen. nov., sp. nov. (YIM 75000 T  = DSM 45377 T  = KCTC 49228 T  = CGMCC 1.13844 T ) which is the nomenclature type of the novel genus Vallicoccus gen. nov. within Vallicoccaceae fam. nov and Motilibacterales ord. nov in the phylum Actinobacteria. The family Vallicoccaceae fam. nov. and the order Motilibacterales (contains Vallicoccaceae fam. nov. and Motilibacteraceae Lee 2013 ) ord. nov. are formally proposed.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s10482-020-01484-5</doi><tpages>11</tpages></addata></record>
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subjects Actinobacteria
Alanine
Arabinose
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
Fatty acids
Gene sequencing
Genomes
Glutamic acid
Glycine
Gram stain
Hybridization
Life Sciences
Mannose
Medical Microbiology
Menaquinones
Microbiology
New species
Nucleotides
Original Paper
Peptidoglycans
pH effects
Phylogenetics
Phylogeny
Plant Sciences
Rhamnose
Ribose
rRNA 16S
Sodium chloride
Soil Science & Conservation
Sugar
title Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov
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