Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis
Visceral leishmaniasis is an endemic protozoonosis observed in over 60 countries, with over 500 000 new cases recorded annually. Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus...
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| Veröffentlicht in: | Parasitology 2021-01, Vol.148 (1), p.110-114 |
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| creator | Pereira, Loren Queli Ferreira-Silva, Márcia Maria Ratkevicius, Cristhianne Molinero Andrade Gómez-Hérnandez, César De Vito, Fernanda Bernadelli Tanaka, Sarah Cristina Sato Vaz Rodrigues Júnior, Virmondes Moraes-Souza, Helio |
| description | Visceral leishmaniasis is an endemic protozoonosis observed in over 60 countries, with over 500 000 new cases recorded annually. Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. Complimentary samples of the ELISA rK39 and conventional PCR showed only reasonable and low agreement with qPCR, respectively. The qPCR confirmed the presence of infection in five of the 10 sequenced samples, ELISA confirmed three, and the conventional PCR confirmed none. |
| doi_str_mv | 10.1017/S0031182020001936 |
| format | Article |
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Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. Complimentary samples of the ELISA rK39 and conventional PCR showed only reasonable and low agreement with qPCR, respectively. The qPCR confirmed the presence of infection in five of the 10 sequenced samples, ELISA confirmed three, and the conventional PCR confirmed none.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182020001936</identifier><identifier>PMID: 33143775</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Agreements ; Animals ; Antibodies, Protozoan - blood ; Asymptomatic ; Asymptomatic infection ; Asymptomatic Infections ; Blood ; Blood & organ donations ; Blood Donors ; Brazil ; Cytochrome ; Cytochrome b ; Cytochromes ; Cytochromes b - genetics ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; DNA, Protozoan - genetics ; Enzyme-Linked Immunosorbent Assay ; Enzymes ; Humans ; Identification methods ; Infections ; Laboratories ; Leishmania infantum ; Leishmania infantum - genetics ; Leishmania infantum - immunology ; Leishmania infantum - isolation & purification ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - immunology ; Parasitic diseases ; Phylogenetics ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Serology ; Vector-borne diseases ; Visceral leishmaniasis ; Zoonoses - diagnosis ; Zoonoses - immunology</subject><ispartof>Parasitology, 2021-01, Vol.148 (1), p.110-114</ispartof><rights>Copyright © The Author(s), 2020. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-958d7e0cb5a7de4027de9fd134680b47f4659d31603bfa0213e6473ee614c35a3</citedby><cites>FETCH-LOGICAL-c373t-958d7e0cb5a7de4027de9fd134680b47f4659d31603bfa0213e6473ee614c35a3</cites><orcidid>0000-0002-7407-4972 ; 0000-0002-8401-0147 ; 0000-0003-2792-8485 ; 0000-0003-4466-6093 ; 0000-0001-8706-4223 ; 0000-0002-8477-4710 ; 0000-0003-2891-443X ; 0000-0003-3469-0022</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182020001936/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33143775$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pereira, Loren Queli</creatorcontrib><creatorcontrib>Ferreira-Silva, Márcia Maria</creatorcontrib><creatorcontrib>Ratkevicius, Cristhianne Molinero Andrade</creatorcontrib><creatorcontrib>Gómez-Hérnandez, César</creatorcontrib><creatorcontrib>De Vito, Fernanda Bernadelli</creatorcontrib><creatorcontrib>Tanaka, Sarah Cristina Sato Vaz</creatorcontrib><creatorcontrib>Rodrigues Júnior, Virmondes</creatorcontrib><creatorcontrib>Moraes-Souza, Helio</creatorcontrib><title>Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Visceral leishmaniasis is an endemic protozoonosis observed in over 60 countries, with over 500 000 new cases recorded annually. Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. Complimentary samples of the ELISA rK39 and conventional PCR showed only reasonable and low agreement with qPCR, respectively. The qPCR confirmed the presence of infection in five of the 10 sequenced samples, ELISA confirmed three, and the conventional PCR confirmed none.</description><subject>Agreements</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Asymptomatic</subject><subject>Asymptomatic infection</subject><subject>Asymptomatic Infections</subject><subject>Blood</subject><subject>Blood & organ donations</subject><subject>Blood Donors</subject><subject>Brazil</subject><subject>Cytochrome</subject><subject>Cytochrome b</subject><subject>Cytochromes</subject><subject>Cytochromes b - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>DNA</subject><subject>DNA, Protozoan - genetics</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Humans</subject><subject>Identification methods</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - immunology</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Leishmaniasis, Visceral - immunology</subject><subject>Parasitic diseases</subject><subject>Phylogenetics</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Sensitivity and Specificity</subject><subject>Serology</subject><subject>Vector-borne diseases</subject><subject>Visceral leishmaniasis</subject><subject>Zoonoses - diagnosis</subject><subject>Zoonoses - immunology</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kcFO3DAQhq2qqCzbPkAvyFIvvQQ8sWMnR4TastJKHIBz5MRj6lVig51U4u1xtAtIVFxsy_P9n0ceQr4DOwMG6vyGMQ5Ql6xkjEHD5SeyAiGbogYJn8lqKRdL_ZicpLTLkOSy_EKOOQfBlapWBDcG_eSs6_XkgqfB0i269HfU3mnqvNV-msd8oN0QgqEm-BATtTGMFL3B0fU04n2O5ssQ6T-Xeox6oMOrJbn0lRxZPST8dtjX5O73r9vLq2J7_WdzebEteq74VDRVbRSyvqu0MihYmdfGGuBC1qwTygpZNYaDZLyzmpXAUQrFESWInlear8nPvfchhscZ09SOSz_DoD2GObWlqJSs6zIL1uTHO3QX5uhzd5lSCiq2p2BP9TGkFNG2D9GNOj61wNplBu1_M8iZ04N57kY0r4mXT88AP0j12EVn7vHt7Y-1z-iDkE4</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Pereira, Loren Queli</creator><creator>Ferreira-Silva, Márcia Maria</creator><creator>Ratkevicius, Cristhianne Molinero Andrade</creator><creator>Gómez-Hérnandez, César</creator><creator>De Vito, Fernanda Bernadelli</creator><creator>Tanaka, Sarah Cristina Sato Vaz</creator><creator>Rodrigues Júnior, Virmondes</creator><creator>Moraes-Souza, Helio</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7407-4972</orcidid><orcidid>https://orcid.org/0000-0002-8401-0147</orcidid><orcidid>https://orcid.org/0000-0003-2792-8485</orcidid><orcidid>https://orcid.org/0000-0003-4466-6093</orcidid><orcidid>https://orcid.org/0000-0001-8706-4223</orcidid><orcidid>https://orcid.org/0000-0002-8477-4710</orcidid><orcidid>https://orcid.org/0000-0003-2891-443X</orcidid><orcidid>https://orcid.org/0000-0003-3469-0022</orcidid></search><sort><creationdate>202101</creationdate><title>Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis</title><author>Pereira, Loren Queli ; Ferreira-Silva, Márcia Maria ; Ratkevicius, Cristhianne Molinero Andrade ; Gómez-Hérnandez, César ; De Vito, Fernanda Bernadelli ; Tanaka, Sarah Cristina Sato Vaz ; Rodrigues Júnior, Virmondes ; Moraes-Souza, Helio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-958d7e0cb5a7de4027de9fd134680b47f4659d31603bfa0213e6473ee614c35a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agreements</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Asymptomatic</topic><topic>Asymptomatic infection</topic><topic>Asymptomatic Infections</topic><topic>Blood</topic><topic>Blood & organ donations</topic><topic>Blood Donors</topic><topic>Brazil</topic><topic>Cytochrome</topic><topic>Cytochrome b</topic><topic>Cytochromes</topic><topic>Cytochromes b - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>DNA</topic><topic>DNA, Protozoan - genetics</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Enzymes</topic><topic>Humans</topic><topic>Identification methods</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - immunology</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Leishmaniasis, Visceral - diagnosis</topic><topic>Leishmaniasis, Visceral - immunology</topic><topic>Parasitic diseases</topic><topic>Phylogenetics</topic><topic>Polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Sensitivity and Specificity</topic><topic>Serology</topic><topic>Vector-borne diseases</topic><topic>Visceral leishmaniasis</topic><topic>Zoonoses - 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Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. 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| subjects | Agreements Animals Antibodies, Protozoan - blood Asymptomatic Asymptomatic infection Asymptomatic Infections Blood Blood & organ donations Blood Donors Brazil Cytochrome Cytochrome b Cytochromes Cytochromes b - genetics Deoxyribonucleic acid Diagnostic systems DNA DNA, Protozoan - genetics Enzyme-Linked Immunosorbent Assay Enzymes Humans Identification methods Infections Laboratories Leishmania infantum Leishmania infantum - genetics Leishmania infantum - immunology Leishmania infantum - isolation & purification Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - immunology Parasitic diseases Phylogenetics Polymerase chain reaction Real-Time Polymerase Chain Reaction Sensitivity and Specificity Serology Vector-borne diseases Visceral leishmaniasis Zoonoses - diagnosis Zoonoses - immunology |
| title | Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis |
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