Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans
This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of Streptococcus mutans in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using S. mutans species and serotype-specific primers. For...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2020-12, Vol.104 (24), p.10601-10612 |
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| creator | Saltos Rosero, Nancy Torres Corral, Yolanda Fernández Álvarez, Clara Aguilera Guirao, Antonio Seoane Prado, Rafael Santos, Ysabel |
| description | This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of
Streptococcus mutans
in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using
S. mutans
species and serotype-specific primers. For bacterial identification, mass spectra data from
S. mutans
and other streptococci were compared with Biotyper V 3.1 database and the mass peak lists were examined by cluster and principal component (PCA) analysis. Identification of potential biomarkers was performed using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and BLAST tool of the NCBI database. PCR identified 100% of the isolates as
S. mutans
.
S. mutans
strains were typed as serotypes c (85.6%), e (8.6%), k (4.8%), and f (0.9%). Although only the 70% of the strains tested were identified at species level by the Biotyper database, PCA and cluster analysis of mass peaks allowed the identification of 100%
S. mutans
isolates and its differentiation from the other oral and non-oral streptococci. One mass peak at
m/z
value of 9572.73 was identified as species-specific biomarker for
S. mutans
. No biomarkers were identified for
S. mutans
serotypes.
Key points
•
MALDI-TOF MS coupled with statistical tools for the identification of S. mutans.
•
Detection of species identifying biomarkers by MALDI-TOF MS.
•
PCR identification and serotyping of S. mutans from saliva samples. |
| doi_str_mv | 10.1007/s00253-020-10980-7 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2457283472</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A642125905</galeid><sourcerecordid>A642125905</sourcerecordid><originalsourceid>FETCH-LOGICAL-c464t-80f43ad37e19f0a135ec799676d0d777fe26fa892b5b8a7b89f55d7520c06e53</originalsourceid><addsrcrecordid>eNp9ktFqFDEUhgdR7Fp9AS9kwBu9mPYkmUxmLkuxWigItl6HbOZkmzIzWXMy0PoGvnUz3WpZEQkhIfn-PyfJXxRvGRwxAHVMAFyKCjhUDLoWKvWsWLFa8AoaVj8vVsCUrJTs2oPiFdENAONt07wsDoRgNeOdWhW_vhO6eZiQqAyuHE2K_rYyRJ4S9uVgCGPZI4W4TT5MZe7-p1mmx8mPuGjc4DfXKUuzBW3RphhGTPGudCGW6RpL3-OUvPP2QbdILlPEbQo2WDtTOc7JTPS6eOHMQPjmcTwsrs4-XZ1-qS6-fj4_PbmobN3UqWrB1cL0QiHrHBgmJFrVdY1qeuiVUg5540zb8bVct0at285J2SvJwUKDUhwWH3a22xh-zEhJj54sDoOZMMykeS0Vb0WteEbf_4XehDlOubhMKZbfOP_CE7UxA2o_uZCisYupPmlqzrjsYDn26B9Ubj2O3oYJnc_re4KPe4LMJLxNGzMT6fPLb_ss37E2BqKITm-jH0280wz0EhW9i4rOUdEPUdFL3e8ebzevR-z_SH5nIwNiB1DemjYYn67_H9t7YhnJog</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2471614007</pqid></control><display><type>article</type><title>Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Saltos Rosero, Nancy ; Torres Corral, Yolanda ; Fernández Álvarez, Clara ; Aguilera Guirao, Antonio ; Seoane Prado, Rafael ; Santos, Ysabel</creator><creatorcontrib>Saltos Rosero, Nancy ; Torres Corral, Yolanda ; Fernández Álvarez, Clara ; Aguilera Guirao, Antonio ; Seoane Prado, Rafael ; Santos, Ysabel</creatorcontrib><description>This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of
Streptococcus mutans
in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using
S. mutans
species and serotype-specific primers. For bacterial identification, mass spectra data from
S. mutans
and other streptococci were compared with Biotyper V 3.1 database and the mass peak lists were examined by cluster and principal component (PCA) analysis. Identification of potential biomarkers was performed using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and BLAST tool of the NCBI database. PCR identified 100% of the isolates as
S. mutans
.
S. mutans
strains were typed as serotypes c (85.6%), e (8.6%), k (4.8%), and f (0.9%). Although only the 70% of the strains tested were identified at species level by the Biotyper database, PCA and cluster analysis of mass peaks allowed the identification of 100%
S. mutans
isolates and its differentiation from the other oral and non-oral streptococci. One mass peak at
m/z
value of 9572.73 was identified as species-specific biomarker for
S. mutans
. No biomarkers were identified for
S. mutans
serotypes.
Key points
•
MALDI-TOF MS coupled with statistical tools for the identification of S. mutans.
•
Detection of species identifying biomarkers by MALDI-TOF MS.
•
PCR identification and serotyping of S. mutans from saliva samples.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-020-10980-7</identifier><identifier>PMID: 33141297</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomarkers ; Biomedical and Life Sciences ; Biotechnology ; Cluster analysis ; Genomics ; Identification ; Identification and classification ; Ionization ; Ions ; Lasers ; Life Sciences ; Mass spectra ; Mass spectrometry ; Mass spectroscopy ; Microbial Genetics and Genomics ; Microbiology ; Plasma desorption mass spectrometry ; Principal components analysis ; Proteomics ; Reliability analysis ; Reproducibility of Results ; Saliva ; Serotypes ; Serotyping ; Species ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Statistical methods ; Statistics ; Strains (organisms) ; Streptococcus ; Streptococcus infections ; Streptococcus mutans ; Streptococcus mutans - genetics ; Time-of-flight mass spectrometry ; Transcriptomics</subject><ispartof>Applied microbiology and biotechnology, 2020-12, Vol.104 (24), p.10601-10612</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c464t-80f43ad37e19f0a135ec799676d0d777fe26fa892b5b8a7b89f55d7520c06e53</cites><orcidid>0000-0002-9506-6496 ; 0000-0003-4398-4102</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-020-10980-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-020-10980-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33141297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saltos Rosero, Nancy</creatorcontrib><creatorcontrib>Torres Corral, Yolanda</creatorcontrib><creatorcontrib>Fernández Álvarez, Clara</creatorcontrib><creatorcontrib>Aguilera Guirao, Antonio</creatorcontrib><creatorcontrib>Seoane Prado, Rafael</creatorcontrib><creatorcontrib>Santos, Ysabel</creatorcontrib><title>Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of
Streptococcus mutans
in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using
S. mutans
species and serotype-specific primers. For bacterial identification, mass spectra data from
S. mutans
and other streptococci were compared with Biotyper V 3.1 database and the mass peak lists were examined by cluster and principal component (PCA) analysis. Identification of potential biomarkers was performed using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and BLAST tool of the NCBI database. PCR identified 100% of the isolates as
S. mutans
.
S. mutans
strains were typed as serotypes c (85.6%), e (8.6%), k (4.8%), and f (0.9%). Although only the 70% of the strains tested were identified at species level by the Biotyper database, PCA and cluster analysis of mass peaks allowed the identification of 100%
S. mutans
isolates and its differentiation from the other oral and non-oral streptococci. One mass peak at
m/z
value of 9572.73 was identified as species-specific biomarker for
S. mutans
. No biomarkers were identified for
S. mutans
serotypes.
Key points
•
MALDI-TOF MS coupled with statistical tools for the identification of S. mutans.
•
Detection of species identifying biomarkers by MALDI-TOF MS.
•
PCR identification and serotyping of S. mutans from saliva samples.</description><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cluster analysis</subject><subject>Genomics</subject><subject>Identification</subject><subject>Identification and classification</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Life Sciences</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Plasma desorption mass spectrometry</subject><subject>Principal components analysis</subject><subject>Proteomics</subject><subject>Reliability analysis</subject><subject>Reproducibility of Results</subject><subject>Saliva</subject><subject>Serotypes</subject><subject>Serotyping</subject><subject>Species</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Statistical methods</subject><subject>Statistics</subject><subject>Strains (organisms)</subject><subject>Streptococcus</subject><subject>Streptococcus infections</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - genetics</subject><subject>Time-of-flight mass spectrometry</subject><subject>Transcriptomics</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9ktFqFDEUhgdR7Fp9AS9kwBu9mPYkmUxmLkuxWigItl6HbOZkmzIzWXMy0PoGvnUz3WpZEQkhIfn-PyfJXxRvGRwxAHVMAFyKCjhUDLoWKvWsWLFa8AoaVj8vVsCUrJTs2oPiFdENAONt07wsDoRgNeOdWhW_vhO6eZiQqAyuHE2K_rYyRJ4S9uVgCGPZI4W4TT5MZe7-p1mmx8mPuGjc4DfXKUuzBW3RphhGTPGudCGW6RpL3-OUvPP2QbdILlPEbQo2WDtTOc7JTPS6eOHMQPjmcTwsrs4-XZ1-qS6-fj4_PbmobN3UqWrB1cL0QiHrHBgmJFrVdY1qeuiVUg5540zb8bVct0at285J2SvJwUKDUhwWH3a22xh-zEhJj54sDoOZMMykeS0Vb0WteEbf_4XehDlOubhMKZbfOP_CE7UxA2o_uZCisYupPmlqzrjsYDn26B9Ubj2O3oYJnc_re4KPe4LMJLxNGzMT6fPLb_ss37E2BqKITm-jH0280wz0EhW9i4rOUdEPUdFL3e8ebzevR-z_SH5nIwNiB1DemjYYn67_H9t7YhnJog</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Saltos Rosero, Nancy</creator><creator>Torres Corral, Yolanda</creator><creator>Fernández Álvarez, Clara</creator><creator>Aguilera Guirao, Antonio</creator><creator>Seoane Prado, Rafael</creator><creator>Santos, Ysabel</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9506-6496</orcidid><orcidid>https://orcid.org/0000-0003-4398-4102</orcidid></search><sort><creationdate>20201201</creationdate><title>Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans</title><author>Saltos Rosero, Nancy ; Torres Corral, Yolanda ; Fernández Álvarez, Clara ; Aguilera Guirao, Antonio ; Seoane Prado, Rafael ; Santos, Ysabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-80f43ad37e19f0a135ec799676d0d777fe26fa892b5b8a7b89f55d7520c06e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cluster analysis</topic><topic>Genomics</topic><topic>Identification</topic><topic>Identification and classification</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Life Sciences</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Plasma desorption mass spectrometry</topic><topic>Principal components analysis</topic><topic>Proteomics</topic><topic>Reliability analysis</topic><topic>Reproducibility of Results</topic><topic>Saliva</topic><topic>Serotypes</topic><topic>Serotyping</topic><topic>Species</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Statistical methods</topic><topic>Statistics</topic><topic>Strains (organisms)</topic><topic>Streptococcus</topic><topic>Streptococcus infections</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - genetics</topic><topic>Time-of-flight mass spectrometry</topic><topic>Transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saltos Rosero, Nancy</creatorcontrib><creatorcontrib>Torres Corral, Yolanda</creatorcontrib><creatorcontrib>Fernández Álvarez, Clara</creatorcontrib><creatorcontrib>Aguilera Guirao, Antonio</creatorcontrib><creatorcontrib>Seoane Prado, Rafael</creatorcontrib><creatorcontrib>Santos, Ysabel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saltos Rosero, Nancy</au><au>Torres Corral, Yolanda</au><au>Fernández Álvarez, Clara</au><au>Aguilera Guirao, Antonio</au><au>Seoane Prado, Rafael</au><au>Santos, Ysabel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>104</volume><issue>24</issue><spage>10601</spage><epage>10612</epage><pages>10601-10612</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of
Streptococcus mutans
in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using
S. mutans
species and serotype-specific primers. For bacterial identification, mass spectra data from
S. mutans
and other streptococci were compared with Biotyper V 3.1 database and the mass peak lists were examined by cluster and principal component (PCA) analysis. Identification of potential biomarkers was performed using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and BLAST tool of the NCBI database. PCR identified 100% of the isolates as
S. mutans
.
S. mutans
strains were typed as serotypes c (85.6%), e (8.6%), k (4.8%), and f (0.9%). Although only the 70% of the strains tested were identified at species level by the Biotyper database, PCA and cluster analysis of mass peaks allowed the identification of 100%
S. mutans
isolates and its differentiation from the other oral and non-oral streptococci. One mass peak at
m/z
value of 9572.73 was identified as species-specific biomarker for
S. mutans
. No biomarkers were identified for
S. mutans
serotypes.
Key points
•
MALDI-TOF MS coupled with statistical tools for the identification of S. mutans.
•
Detection of species identifying biomarkers by MALDI-TOF MS.
•
PCR identification and serotyping of S. mutans from saliva samples.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33141297</pmid><doi>10.1007/s00253-020-10980-7</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-9506-6496</orcidid><orcidid>https://orcid.org/0000-0003-4398-4102</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2020-12, Vol.104 (24), p.10601-10612 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2457283472 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Biomarkers Biomedical and Life Sciences Biotechnology Cluster analysis Genomics Identification Identification and classification Ionization Ions Lasers Life Sciences Mass spectra Mass spectrometry Mass spectroscopy Microbial Genetics and Genomics Microbiology Plasma desorption mass spectrometry Principal components analysis Proteomics Reliability analysis Reproducibility of Results Saliva Serotypes Serotyping Species Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Statistical methods Statistics Strains (organisms) Streptococcus Streptococcus infections Streptococcus mutans Streptococcus mutans - genetics Time-of-flight mass spectrometry Transcriptomics |
| title | Usefulness of matrix-assisted laser desorption ionization/time of flight mass spectrometry for the identification of Streptococcus mutans |
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