Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells
This study investigated kimchi‐induced apoptosis in HT‐29 human colon carcinoma cells. Three types of kimchi samples were prepared: standardized kimchi brined with general commercial Baechu cabbage by a standardized recipe (SK), Amtak Baechu kimchi brined with Amtak Baechu cabbage by a standardized...
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| Veröffentlicht in: | Journal of food biochemistry 2021-01, Vol.45 (1), p.e13532-n/a |
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| creator | Yu, Ting Park, Eui‐Seong Song, Gil‐Hoon Zhao, Xin Yi, Ruo‐Kun Park, Kun‐Young |
| description | This study investigated kimchi‐induced apoptosis in HT‐29 human colon carcinoma cells. Three types of kimchi samples were prepared: standardized kimchi brined with general commercial Baechu cabbage by a standardized recipe (SK), Amtak Baechu kimchi brined with Amtak Baechu cabbage by a standardized recipe (AmK), and anticancer kimchi brined with organically cultivated Baechu cabbage by a functional recipe (AK). MTT assay, qRT‐PCR, and Western blotting analysis were performed. The results indicate that AmK and AK, especially AK significantly upregulated mRNA expression of apoptosis‐related genes Bim, Bax, Bak, caspase‐8, ‐9, ‐3, and p53 but suppressed Bcl‐xL and Bcl‐2 expression. In addition, AK treatment significantly upregulated protein expression levels of caspase‐3 but strikingly reduced the protein expression level of Bcl‐2 (p |
| doi_str_mv | 10.1111/jfbc.13532 |
| format | Article |
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Practical applications
Colon cancer is the fourth cancer with the highest incidence in the world. Cell apoptosis is a type of programmed cell death and plays an important role in the cancer cells study. Kimchi is a traditional fermented food in Korea, with a relatively high daily consumption. Our present study used three kinds of kimchi which prepared with different main ingredients and recipes. The results suggest that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anticancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.
Cell apoptosis is closely related to cancer research. Kimchi is a traditional fermented functional food in South Korea, which has a high daily consumption. Therefore, it is of great significance to develop kimchi with anticancer function. Our present study suggests that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anti‐cancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.</description><identifier>ISSN: 0145-8884</identifier><identifier>EISSN: 1745-4514</identifier><identifier>DOI: 10.1111/jfbc.13532</identifier><identifier>PMID: 33140497</identifier><language>eng</language><publisher>United States</publisher><subject>Bcl‐2 family ; caspases ; fermented food</subject><ispartof>Journal of food biochemistry, 2021-01, Vol.45 (1), p.e13532-n/a</ispartof><rights>2020 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3652-23af99760c9a6d2036b383d38d4096484a8368464547bb3db1a777303689571f3</citedby><cites>FETCH-LOGICAL-c3652-23af99760c9a6d2036b383d38d4096484a8368464547bb3db1a777303689571f3</cites><orcidid>0000-0002-3644-7467 ; 0000-0001-9798-7865 ; 0000-0002-2674-0715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfbc.13532$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfbc.13532$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33140497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Ting</creatorcontrib><creatorcontrib>Park, Eui‐Seong</creatorcontrib><creatorcontrib>Song, Gil‐Hoon</creatorcontrib><creatorcontrib>Zhao, Xin</creatorcontrib><creatorcontrib>Yi, Ruo‐Kun</creatorcontrib><creatorcontrib>Park, Kun‐Young</creatorcontrib><title>Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells</title><title>Journal of food biochemistry</title><addtitle>J Food Biochem</addtitle><description>This study investigated kimchi‐induced apoptosis in HT‐29 human colon carcinoma cells. Three types of kimchi samples were prepared: standardized kimchi brined with general commercial Baechu cabbage by a standardized recipe (SK), Amtak Baechu kimchi brined with Amtak Baechu cabbage by a standardized recipe (AmK), and anticancer kimchi brined with organically cultivated Baechu cabbage by a functional recipe (AK). MTT assay, qRT‐PCR, and Western blotting analysis were performed. The results indicate that AmK and AK, especially AK significantly upregulated mRNA expression of apoptosis‐related genes Bim, Bax, Bak, caspase‐8, ‐9, ‐3, and p53 but suppressed Bcl‐xL and Bcl‐2 expression. In addition, AK treatment significantly upregulated protein expression levels of caspase‐3 but strikingly reduced the protein expression level of Bcl‐2 (p < .05), followed by AmK treatment. Our data suggest that AK and AmK can markedly suppress the proliferation of HT‐29 cells via activation of apoptosis.
Practical applications
Colon cancer is the fourth cancer with the highest incidence in the world. Cell apoptosis is a type of programmed cell death and plays an important role in the cancer cells study. Kimchi is a traditional fermented food in Korea, with a relatively high daily consumption. Our present study used three kinds of kimchi which prepared with different main ingredients and recipes. The results suggest that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anticancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.
Cell apoptosis is closely related to cancer research. Kimchi is a traditional fermented functional food in South Korea, which has a high daily consumption. Therefore, it is of great significance to develop kimchi with anticancer function. Our present study suggests that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anti‐cancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.</description><subject>Bcl‐2 family</subject><subject>caspases</subject><subject>fermented food</subject><issn>0145-8884</issn><issn>1745-4514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kL1OwzAURi0EoqWw8AAoI0JK8V9sZ4SIUqASS5ktx3FUlyQOcSPUjUfgGXkSXFIY8WBfXR0dff4AOEdwisK5Xpe5niKSEHwAxojTJKYJoodgDFGYhRB0BE68X0MIccroMRgRgiikKR-D-ZOt9cpGtepeTVFtI9sUvTY-Uq1rN85bHzbRfPn18YnTaNXXqom0q1y4Vadt42oVaVNV_hQclary5mz_TsDL7G6ZzePF8_1DdrOINWEJjjFRZZpyBnWqWIEhYTkRpCCioDBkE1QJwgRlNKE8z0mRI8U5J4ETacJRSSbgcvC2nXvrjd_I2vpdAtUY13uJacIxZyKoJ-BqQHXnvO9MKdvOho9uJYJy15zcNSd_mgvwxd7b57Up_tDfqgKABuDdVmb7j0o-zm6zQfoNBbt20A</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Yu, Ting</creator><creator>Park, Eui‐Seong</creator><creator>Song, Gil‐Hoon</creator><creator>Zhao, Xin</creator><creator>Yi, Ruo‐Kun</creator><creator>Park, Kun‐Young</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3644-7467</orcidid><orcidid>https://orcid.org/0000-0001-9798-7865</orcidid><orcidid>https://orcid.org/0000-0002-2674-0715</orcidid></search><sort><creationdate>202101</creationdate><title>Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells</title><author>Yu, Ting ; Park, Eui‐Seong ; Song, Gil‐Hoon ; Zhao, Xin ; Yi, Ruo‐Kun ; Park, Kun‐Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3652-23af99760c9a6d2036b383d38d4096484a8368464547bb3db1a777303689571f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bcl‐2 family</topic><topic>caspases</topic><topic>fermented food</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Ting</creatorcontrib><creatorcontrib>Park, Eui‐Seong</creatorcontrib><creatorcontrib>Song, Gil‐Hoon</creatorcontrib><creatorcontrib>Zhao, Xin</creatorcontrib><creatorcontrib>Yi, Ruo‐Kun</creatorcontrib><creatorcontrib>Park, Kun‐Young</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Ting</au><au>Park, Eui‐Seong</au><au>Song, Gil‐Hoon</au><au>Zhao, Xin</au><au>Yi, Ruo‐Kun</au><au>Park, Kun‐Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells</atitle><jtitle>Journal of food biochemistry</jtitle><addtitle>J Food Biochem</addtitle><date>2021-01</date><risdate>2021</risdate><volume>45</volume><issue>1</issue><spage>e13532</spage><epage>n/a</epage><pages>e13532-n/a</pages><issn>0145-8884</issn><eissn>1745-4514</eissn><abstract>This study investigated kimchi‐induced apoptosis in HT‐29 human colon carcinoma cells. Three types of kimchi samples were prepared: standardized kimchi brined with general commercial Baechu cabbage by a standardized recipe (SK), Amtak Baechu kimchi brined with Amtak Baechu cabbage by a standardized recipe (AmK), and anticancer kimchi brined with organically cultivated Baechu cabbage by a functional recipe (AK). MTT assay, qRT‐PCR, and Western blotting analysis were performed. The results indicate that AmK and AK, especially AK significantly upregulated mRNA expression of apoptosis‐related genes Bim, Bax, Bak, caspase‐8, ‐9, ‐3, and p53 but suppressed Bcl‐xL and Bcl‐2 expression. In addition, AK treatment significantly upregulated protein expression levels of caspase‐3 but strikingly reduced the protein expression level of Bcl‐2 (p < .05), followed by AmK treatment. Our data suggest that AK and AmK can markedly suppress the proliferation of HT‐29 cells via activation of apoptosis.
Practical applications
Colon cancer is the fourth cancer with the highest incidence in the world. Cell apoptosis is a type of programmed cell death and plays an important role in the cancer cells study. Kimchi is a traditional fermented food in Korea, with a relatively high daily consumption. Our present study used three kinds of kimchi which prepared with different main ingredients and recipes. The results suggest that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anticancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.
Cell apoptosis is closely related to cancer research. Kimchi is a traditional fermented functional food in South Korea, which has a high daily consumption. Therefore, it is of great significance to develop kimchi with anticancer function. Our present study suggests that organically cultivated Baechu cabbage and functional recipe in kimchi preparation play an important role in the anti‐cancer efficacy of kimchi, which has been shown to promote induction of apoptosis in HT‐29 cells.</abstract><cop>United States</cop><pmid>33140497</pmid><doi>10.1111/jfbc.13532</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0002-3644-7467</orcidid><orcidid>https://orcid.org/0000-0001-9798-7865</orcidid><orcidid>https://orcid.org/0000-0002-2674-0715</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bcl‐2 family caspases fermented food |
| title | Kimchi markedly induces apoptosis in HT‐29 human colon carcinoma cells |
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