Fluorescence Discrimination of Pharmacological Effects on the Na+–Ca2+ Exchanger and Sarco-Endoplasmic Reticulum Ca2+-ATPase in Mouse Ventricular Cardiomyocytes
We developed a method to evaluate the activity of the Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decreas...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2021/01/01, Vol.44(1), pp.154-157 |
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| creator | Namekata, Iyuki Odaka, Ryosuke Hamazaki, Shunsuke Nisaka, Hina Hamaguchi, Shogo Tanaka, Hikaru |
| description | We developed a method to evaluate the activity of the Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by SEA0400, an NCX inhibitor, but not cyclopiazonic acid, a SERCA inhibitor. β-Adrenoceptor stimulation by isoprenaline caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by cyclopiazonic acid but not SEA0400. Ellagic acid, a phenolic phytochemical, also decreased the basal Ca2+ concentration, which was inhibited by cyclopiazonic acid, but not SEA0400. Thus, this method using fluorescent microscopy and specific inhibitors would be useful for the evaluation of pharmacological agents acting on NCX and SERCA. |
| doi_str_mv | 10.1248/bpb.b20-00701 |
| format | Article |
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In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by SEA0400, an NCX inhibitor, but not cyclopiazonic acid, a SERCA inhibitor. β-Adrenoceptor stimulation by isoprenaline caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by cyclopiazonic acid but not SEA0400. Ellagic acid, a phenolic phytochemical, also decreased the basal Ca2+ concentration, which was inhibited by cyclopiazonic acid, but not SEA0400. Thus, this method using fluorescent microscopy and specific inhibitors would be useful for the evaluation of pharmacological agents acting on NCX and SERCA.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b20-00701</identifier><identifier>PMID: 33132367</identifier><language>eng</language><publisher>TOKYO: The Pharmaceutical Society of Japan</publisher><subject>Acids ; Adrenergic receptors ; Ca2+ imaging ; Ca2+-transporting ATPase ; Calcium (reticular) ; Cardiomyocytes ; Cyclopiazonic acid ; Ellagic acid ; Endoplasmic reticulum ; Fluorescence microscopy ; Life Sciences & Biomedicine ; Microscopy ; Na+/Ca2+ exchanger ; Pharmacology & Pharmacy ; Phenylephrine ; sarco-endoplasmic reticulum Ca2+-ATPase ; Science & Technology ; Ventricle</subject><ispartof>Biological and Pharmaceutical Bulletin, 2021/01/01, Vol.44(1), pp.154-157</ispartof><rights>2021 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>3</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000620798500025</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c570t-213be9cff8ad5bb49b4d7c31942b37b5013e33afb2f449632e46d4d77e3409713</citedby><cites>FETCH-LOGICAL-c570t-213be9cff8ad5bb49b4d7c31942b37b5013e33afb2f449632e46d4d77e3409713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27928,27929</link.rule.ids></links><search><creatorcontrib>Namekata, Iyuki</creatorcontrib><creatorcontrib>Odaka, Ryosuke</creatorcontrib><creatorcontrib>Hamazaki, Shunsuke</creatorcontrib><creatorcontrib>Nisaka, Hina</creatorcontrib><creatorcontrib>Hamaguchi, Shogo</creatorcontrib><creatorcontrib>Tanaka, Hikaru</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>Faculty of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Toho University</creatorcontrib><title>Fluorescence Discrimination of Pharmacological Effects on the Na+–Ca2+ Exchanger and Sarco-Endoplasmic Reticulum Ca2+-ATPase in Mouse Ventricular Cardiomyocytes</title><title>Biological & pharmaceutical bulletin</title><addtitle>BIOL PHARM BULL</addtitle><description>We developed a method to evaluate the activity of the Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by SEA0400, an NCX inhibitor, but not cyclopiazonic acid, a SERCA inhibitor. β-Adrenoceptor stimulation by isoprenaline caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by cyclopiazonic acid but not SEA0400. Ellagic acid, a phenolic phytochemical, also decreased the basal Ca2+ concentration, which was inhibited by cyclopiazonic acid, but not SEA0400. Thus, this method using fluorescent microscopy and specific inhibitors would be useful for the evaluation of pharmacological agents acting on NCX and SERCA.</description><subject>Acids</subject><subject>Adrenergic receptors</subject><subject>Ca2+ imaging</subject><subject>Ca2+-transporting ATPase</subject><subject>Calcium (reticular)</subject><subject>Cardiomyocytes</subject><subject>Cyclopiazonic acid</subject><subject>Ellagic acid</subject><subject>Endoplasmic reticulum</subject><subject>Fluorescence microscopy</subject><subject>Life Sciences & Biomedicine</subject><subject>Microscopy</subject><subject>Na+/Ca2+ exchanger</subject><subject>Pharmacology & Pharmacy</subject><subject>Phenylephrine</subject><subject>sarco-endoplasmic reticulum Ca2+-ATPase</subject><subject>Science & Technology</subject><subject>Ventricle</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNks2O0zAQgCMEYsvCkbslLkhVFv8lTo6r0F2QFljBwtWynUnrKrGLnQh64x14Ax6NJ8FpUVfixME_kr8Zz_hzlj0n-IJQXr3SO32hKc4xFpg8yBaEcZEXlBQPswWuSZWXpKjOsicxbvHMUPY4O2OMMMpKsch-XfWTDxANOAPotY0m2ME6NVrvkO_Q7UaFQRnf-7U1qkerrgMzRpROxw2g92r5-8fPRtElWn03G-XWEJByLfqkgvH5yrV-16s4WIM-wmjN1E8DmvH88u5WRUDWoXd-Spsv4MYwAyokILTWD3tv9iPEp9mjTvURnv1dz7PPV6u75k1-8-H6bXN5k5tC4DGnhGmoTddVqi205rXmrTCM1JxqJnSBCQPGVKdpx3ldMgq8bBMigHFcC8LOs5fHvLvgv04QRzmk54C-Vw5SiZLyoqwKkUZCX_yDbv0UXKouUSJxZVHjROVHygQfY4BO7tLjqrCXBMtZnkzyZJInD_ISvzzy30D7Lho7OznFJHslxaKuirSjcw3V_9ONHQ9KGz-5MYVeH0MHaGer3vXWwX0PJgptk3JJMSUpFec4LbSQmBR8ngQTpMJsLrk5ZtrGUa3vr1chye7h0CDnkszTqdHTafowQYJjfwDNZtvd</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Namekata, Iyuki</creator><creator>Odaka, Ryosuke</creator><creator>Hamazaki, Shunsuke</creator><creator>Nisaka, Hina</creator><creator>Hamaguchi, Shogo</creator><creator>Tanaka, Hikaru</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Pharmaceutical Soc Japan</general><general>Japan Science and Technology Agency</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210101</creationdate><title>Fluorescence Discrimination of Pharmacological Effects on the Na+–Ca2+ Exchanger and Sarco-Endoplasmic Reticulum Ca2+-ATPase in Mouse Ventricular Cardiomyocytes</title><author>Namekata, Iyuki ; 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In non-beating ventricular cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by SEA0400, an NCX inhibitor, but not cyclopiazonic acid, a SERCA inhibitor. β-Adrenoceptor stimulation by isoprenaline caused a decrease in the cytoplasmic Ca2+ concentration, which was inhibited by cyclopiazonic acid but not SEA0400. Ellagic acid, a phenolic phytochemical, also decreased the basal Ca2+ concentration, which was inhibited by cyclopiazonic acid, but not SEA0400. Thus, this method using fluorescent microscopy and specific inhibitors would be useful for the evaluation of pharmacological agents acting on NCX and SERCA.</abstract><cop>TOKYO</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>33132367</pmid><doi>10.1248/bpb.b20-00701</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Adrenergic receptors Ca2+ imaging Ca2+-transporting ATPase Calcium (reticular) Cardiomyocytes Cyclopiazonic acid Ellagic acid Endoplasmic reticulum Fluorescence microscopy Life Sciences & Biomedicine Microscopy Na+/Ca2+ exchanger Pharmacology & Pharmacy Phenylephrine sarco-endoplasmic reticulum Ca2+-ATPase Science & Technology Ventricle |
| title | Fluorescence Discrimination of Pharmacological Effects on the Na+–Ca2+ Exchanger and Sarco-Endoplasmic Reticulum Ca2+-ATPase in Mouse Ventricular Cardiomyocytes |
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