Clusterin is upregulated in serum and muscle tissue in idiopathic inflammatory myopathies and associates with clinical disease activity and cytokine profile
The aim of this cross-sectional study was to explore the circulating and skeletal muscle expression of clusterin (CLU) in inflammatory myopathies (IIM) and its potential implication in pathogenetic mechanisms of the disease. A total of 85 IIM patients and 86 healthy controls (HC) were recruited. In...
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| Veröffentlicht in: | Clinical and Experimental Rheumatology 2021-09, Vol.39 (5), p.1021-1032 |
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| creator | Kropáčková, Tereza Vernerová, Lucia Štorkánová, Hana Horváthová, Veronika Vokurková, Martina Klein, Martin Oreská, Sabína Špiritović, Maja Heřmánková, Barbora Kubínová, Kateřina Cerezo, Lucie Andrés Kryštůfková, Olga Mann, Heřman Ukropec, Jozef Ukropcová, Barbara Zámečník, Josef Tomčík, Michal Vencovský, Jiří Šenolt, Ladislav |
| description | The aim of this cross-sectional study was to explore the circulating and skeletal muscle expression of clusterin (CLU) in inflammatory myopathies (IIM) and its potential implication in pathogenetic mechanisms of the disease.
A total of 85 IIM patients and 86 healthy controls (HC) were recruited. In addition, 20 IIM patients and 21 HC underwent a muscle biopsy. Circulating CLU was measured by ELISA. Serum cytokine profile of patients and HC was assessed by Cytokine 27-plex Assay. Immunohistochemical localisation of CLU was assessed in 10 IIM and 4 control muscle tissue specimens. The expression of CLU and myositis related cytokines in muscle was determined by qPCR.
Serum levels of CLU were significantly increased in IIM patients compared to controls (86.2 (71.6-99.0) vs. 59.6 (52.6-68.4) μg/mL, p |
| doi_str_mv | 10.55563/clinexprheumatol/k3vtg5 |
| format | Article |
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A total of 85 IIM patients and 86 healthy controls (HC) were recruited. In addition, 20 IIM patients and 21 HC underwent a muscle biopsy. Circulating CLU was measured by ELISA. Serum cytokine profile of patients and HC was assessed by Cytokine 27-plex Assay. Immunohistochemical localisation of CLU was assessed in 10 IIM and 4 control muscle tissue specimens. The expression of CLU and myositis related cytokines in muscle was determined by qPCR.
Serum levels of CLU were significantly increased in IIM patients compared to controls (86.2 (71.6-99.0) vs. 59.6 (52.6-68.4) μg/mL, p<0.0001) and positively correlated with myositis disease activity assessment (MYOACT) (r=0.337, p=0.008), myositis intention-to-treat activity index (MITAX) (r=0.357, p=0.004) and global disease assessment evaluated by physician (r=0.309, p=0.015). Moreover, serum CLU correlated with cytokines and chemokines involved in IIM and their combined effect on disease activity was revealed by multivariate redundancy analysis. In muscle tissue, CLU mRNA was increased in IIM patients compared to controls (p=0.032) and CLU accumulated in the cytoplasm of regenerating myofibres.
We suggest that the up-regulation of clusterin in circulation and skeletal muscle of IIM patients may be an inflammation and atrophy induced response of the organism intended to limit the environment, favouring further muscle damage.</description><identifier>ISSN: 0392-856X</identifier><identifier>EISSN: 1593-098X</identifier><identifier>DOI: 10.55563/clinexprheumatol/k3vtg5</identifier><identifier>PMID: 33124569</identifier><language>eng</language><publisher>Italy</publisher><subject>Clusterin - genetics ; Cross-Sectional Studies ; Cytokines ; Humans ; Muscle, Skeletal ; Myositis</subject><ispartof>Clinical and Experimental Rheumatology, 2021-09, Vol.39 (5), p.1021-1032</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-ac6d4403cac4909ab65788d6de59d4dd1c9153243409fb1c4fd60e12c9f072cb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33124569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kropáčková, Tereza</creatorcontrib><creatorcontrib>Vernerová, Lucia</creatorcontrib><creatorcontrib>Štorkánová, Hana</creatorcontrib><creatorcontrib>Horváthová, Veronika</creatorcontrib><creatorcontrib>Vokurková, Martina</creatorcontrib><creatorcontrib>Klein, Martin</creatorcontrib><creatorcontrib>Oreská, Sabína</creatorcontrib><creatorcontrib>Špiritović, Maja</creatorcontrib><creatorcontrib>Heřmánková, Barbora</creatorcontrib><creatorcontrib>Kubínová, Kateřina</creatorcontrib><creatorcontrib>Cerezo, Lucie Andrés</creatorcontrib><creatorcontrib>Kryštůfková, Olga</creatorcontrib><creatorcontrib>Mann, Heřman</creatorcontrib><creatorcontrib>Ukropec, Jozef</creatorcontrib><creatorcontrib>Ukropcová, Barbara</creatorcontrib><creatorcontrib>Zámečník, Josef</creatorcontrib><creatorcontrib>Tomčík, Michal</creatorcontrib><creatorcontrib>Vencovský, Jiří</creatorcontrib><creatorcontrib>Šenolt, Ladislav</creatorcontrib><title>Clusterin is upregulated in serum and muscle tissue in idiopathic inflammatory myopathies and associates with clinical disease activity and cytokine profile</title><title>Clinical and Experimental Rheumatology</title><addtitle>Clin Exp Rheumatol</addtitle><description>The aim of this cross-sectional study was to explore the circulating and skeletal muscle expression of clusterin (CLU) in inflammatory myopathies (IIM) and its potential implication in pathogenetic mechanisms of the disease.
A total of 85 IIM patients and 86 healthy controls (HC) were recruited. In addition, 20 IIM patients and 21 HC underwent a muscle biopsy. Circulating CLU was measured by ELISA. Serum cytokine profile of patients and HC was assessed by Cytokine 27-plex Assay. Immunohistochemical localisation of CLU was assessed in 10 IIM and 4 control muscle tissue specimens. The expression of CLU and myositis related cytokines in muscle was determined by qPCR.
Serum levels of CLU were significantly increased in IIM patients compared to controls (86.2 (71.6-99.0) vs. 59.6 (52.6-68.4) μg/mL, p<0.0001) and positively correlated with myositis disease activity assessment (MYOACT) (r=0.337, p=0.008), myositis intention-to-treat activity index (MITAX) (r=0.357, p=0.004) and global disease assessment evaluated by physician (r=0.309, p=0.015). Moreover, serum CLU correlated with cytokines and chemokines involved in IIM and their combined effect on disease activity was revealed by multivariate redundancy analysis. In muscle tissue, CLU mRNA was increased in IIM patients compared to controls (p=0.032) and CLU accumulated in the cytoplasm of regenerating myofibres.
We suggest that the up-regulation of clusterin in circulation and skeletal muscle of IIM patients may be an inflammation and atrophy induced response of the organism intended to limit the environment, favouring further muscle damage.</description><subject>Clusterin - genetics</subject><subject>Cross-Sectional Studies</subject><subject>Cytokines</subject><subject>Humans</subject><subject>Muscle, Skeletal</subject><subject>Myositis</subject><issn>0392-856X</issn><issn>1593-098X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUU1v2zAMFYYOa9rtLww67uJGsj5iHYdgXQsU6KUFejMUiW60yrEnSt38X_pj6yTdDj0RJN_jI_kIoZxdKKW0WLoYdvB3TFsovc1DXD6J5_yoPpAFV0ZUzDQPJ2TBhKmrRumHU3KG-IuxWiu9-kROheC1VNosyMs6FsyQwo4GpGVM8FiizeDpXEFIpad252lf0EWgOSAW2LeCD8No8za4Oeui7fdrpIn207EMeOBZxMGFeR7SPyFv6X7v4GykPiBYBGpdDs8hTwe0m_LwNB9GxzR0IcJn8rGzEeHLWzwn95c_7tZX1c3tz-v195vKCa5yZZ32UjLhrJOGGbvRatU0XntQxkvvuTNciVoKyUy34U52XjPgtTMdW9VuI87Jt-PcWfd3AcxtH9BBjHYHQ8F2_yvJTa2bGdocoS4NiAm6dkyht2lqOWsP3rTvvWmP3szUr28qZdOD_0_8Z4Z4BayRl8U</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Kropáčková, Tereza</creator><creator>Vernerová, Lucia</creator><creator>Štorkánová, Hana</creator><creator>Horváthová, Veronika</creator><creator>Vokurková, Martina</creator><creator>Klein, Martin</creator><creator>Oreská, Sabína</creator><creator>Špiritović, Maja</creator><creator>Heřmánková, Barbora</creator><creator>Kubínová, Kateřina</creator><creator>Cerezo, Lucie Andrés</creator><creator>Kryštůfková, Olga</creator><creator>Mann, Heřman</creator><creator>Ukropec, Jozef</creator><creator>Ukropcová, Barbara</creator><creator>Zámečník, Josef</creator><creator>Tomčík, Michal</creator><creator>Vencovský, Jiří</creator><creator>Šenolt, Ladislav</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210901</creationdate><title>Clusterin is upregulated in serum and muscle tissue in idiopathic inflammatory myopathies and associates with clinical disease activity and cytokine profile</title><author>Kropáčková, Tereza ; 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A total of 85 IIM patients and 86 healthy controls (HC) were recruited. In addition, 20 IIM patients and 21 HC underwent a muscle biopsy. Circulating CLU was measured by ELISA. Serum cytokine profile of patients and HC was assessed by Cytokine 27-plex Assay. Immunohistochemical localisation of CLU was assessed in 10 IIM and 4 control muscle tissue specimens. The expression of CLU and myositis related cytokines in muscle was determined by qPCR.
Serum levels of CLU were significantly increased in IIM patients compared to controls (86.2 (71.6-99.0) vs. 59.6 (52.6-68.4) μg/mL, p<0.0001) and positively correlated with myositis disease activity assessment (MYOACT) (r=0.337, p=0.008), myositis intention-to-treat activity index (MITAX) (r=0.357, p=0.004) and global disease assessment evaluated by physician (r=0.309, p=0.015). Moreover, serum CLU correlated with cytokines and chemokines involved in IIM and their combined effect on disease activity was revealed by multivariate redundancy analysis. In muscle tissue, CLU mRNA was increased in IIM patients compared to controls (p=0.032) and CLU accumulated in the cytoplasm of regenerating myofibres.
We suggest that the up-regulation of clusterin in circulation and skeletal muscle of IIM patients may be an inflammation and atrophy induced response of the organism intended to limit the environment, favouring further muscle damage.</abstract><cop>Italy</cop><pmid>33124569</pmid><doi>10.55563/clinexprheumatol/k3vtg5</doi><tpages>12</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Clusterin - genetics Cross-Sectional Studies Cytokines Humans Muscle, Skeletal Myositis |
| title | Clusterin is upregulated in serum and muscle tissue in idiopathic inflammatory myopathies and associates with clinical disease activity and cytokine profile |
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